Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS12200 and ABZR86_RS12205 are separated by 56 nucleotides ABZR86_RS12205 and ABZR86_RS12210 overlap by 4 nucleotides ABZR86_RS12210 and ABZR86_RS12215 are separated by 131 nucleotides
ABZR86_RS12200: ABZR86_RS12200 - ribulose-phosphate 3-epimerase, at 2,727,923 to 2,728,591
_RS12200
ABZR86_RS12205: ABZR86_RS12205 - co-chaperone DjlA, at 2,728,648 to 2,729,451
_RS12205
ABZR86_RS12210: ABZR86_RS12210 - c-type cytochrome, at 2,729,448 to 2,729,759
_RS12210
ABZR86_RS12215: ABZR86_RS12215 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 2,729,891 to 2,730,787
_RS12215
Position (kb)
2728
2729
2730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2727.966 kb on + strand at 2727.966 kb on + strand at 2728.413 kb on + strand, within ABZR86_RS12200 at 2728.414 kb on - strand, within ABZR86_RS12200 at 2728.414 kb on - strand, within ABZR86_RS12200 at 2728.464 kb on + strand, within ABZR86_RS12200 at 2728.464 kb on + strand, within ABZR86_RS12200 at 2728.464 kb on + strand, within ABZR86_RS12200 at 2728.464 kb on + strand, within ABZR86_RS12200 at 2728.464 kb on + strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.465 kb on - strand, within ABZR86_RS12200 at 2728.630 kb on + strand at 2728.630 kb on + strand at 2728.630 kb on + strand at 2728.631 kb on - strand at 2728.631 kb on - strand at 2728.682 kb on + strand at 2728.683 kb on - strand at 2728.683 kb on - strand at 2728.785 kb on - strand, within ABZR86_RS12205 at 2728.838 kb on + strand, within ABZR86_RS12205 at 2728.838 kb on + strand, within ABZR86_RS12205 at 2728.839 kb on - strand, within ABZR86_RS12205 at 2728.839 kb on - strand, within ABZR86_RS12205 at 2728.839 kb on - strand, within ABZR86_RS12205 at 2728.857 kb on + strand, within ABZR86_RS12205 at 2728.877 kb on + strand, within ABZR86_RS12205 at 2728.877 kb on + strand, within ABZR86_RS12205 at 2728.895 kb on + strand, within ABZR86_RS12205 at 2728.910 kb on + strand, within ABZR86_RS12205 at 2728.910 kb on + strand, within ABZR86_RS12205 at 2728.910 kb on + strand, within ABZR86_RS12205 at 2728.911 kb on - strand, within ABZR86_RS12205 at 2728.911 kb on - strand, within ABZR86_RS12205 at 2728.934 kb on + strand, within ABZR86_RS12205 at 2728.935 kb on - strand, within ABZR86_RS12205 at 2729.303 kb on + strand, within ABZR86_RS12205 at 2729.304 kb on - strand, within ABZR86_RS12205 at 2729.304 kb on - strand, within ABZR86_RS12205 at 2729.482 kb on + strand, within ABZR86_RS12210 at 2729.483 kb on - strand, within ABZR86_RS12210 at 2729.593 kb on + strand, within ABZR86_RS12210 at 2729.593 kb on + strand, within ABZR86_RS12210 at 2729.594 kb on - strand, within ABZR86_RS12210 at 2729.594 kb on - strand, within ABZR86_RS12210 at 2729.594 kb on - strand, within ABZR86_RS12210 at 2729.737 kb on - strand at 2729.805 kb on + strand at 2729.805 kb on + strand at 2729.806 kb on - strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.808 kb on + strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.809 kb on - strand at 2729.848 kb on - strand at 2730.173 kb on + strand, within ABZR86_RS12215 at 2730.267 kb on - strand, within ABZR86_RS12215
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 2,727,966 + -2.3 2,727,966 + -2.3 2,728,413 + ABZR86_RS12200 0.73 -0.8 2,728,414 - ABZR86_RS12200 0.73 -1.1 2,728,414 - ABZR86_RS12200 0.73 -2.2 2,728,464 + ABZR86_RS12200 0.81 -1.0 2,728,464 + ABZR86_RS12200 0.81 -1.7 2,728,464 + ABZR86_RS12200 0.81 -0.9 2,728,464 + ABZR86_RS12200 0.81 -1.0 2,728,464 + ABZR86_RS12200 0.81 -2.5 2,728,465 - ABZR86_RS12200 0.81 -1.4 2,728,465 - ABZR86_RS12200 0.81 -3.1 2,728,465 - ABZR86_RS12200 0.81 -1.4 2,728,465 - ABZR86_RS12200 0.81 -2.7 2,728,465 - ABZR86_RS12200 0.81 -1.3 2,728,465 - ABZR86_RS12200 0.81 -3.0 2,728,465 - ABZR86_RS12200 0.81 -2.2 2,728,465 - ABZR86_RS12200 0.81 -2.4 2,728,630 + -0.4 2,728,630 + +0.6 2,728,630 + -2.4 2,728,631 - -0.3 2,728,631 - +0.5 2,728,682 + -2.5 2,728,683 - -2.0 2,728,683 - -1.0 2,728,785 - ABZR86_RS12205 0.17 -3.6 2,728,838 + ABZR86_RS12205 0.24 -2.9 2,728,838 + ABZR86_RS12205 0.24 -2.3 2,728,839 - ABZR86_RS12205 0.24 -3.4 2,728,839 - ABZR86_RS12205 0.24 -3.7 2,728,839 - ABZR86_RS12205 0.24 -2.9 2,728,857 + ABZR86_RS12205 0.26 -1.0 2,728,877 + ABZR86_RS12205 0.28 -2.1 2,728,877 + ABZR86_RS12205 0.28 -2.5 2,728,895 + ABZR86_RS12205 0.31 -1.6 2,728,910 + ABZR86_RS12205 0.33 -1.6 2,728,910 + ABZR86_RS12205 0.33 -2.6 2,728,910 + ABZR86_RS12205 0.33 -2.2 2,728,911 - ABZR86_RS12205 0.33 -2.4 2,728,911 - ABZR86_RS12205 0.33 -0.4 2,728,934 + ABZR86_RS12205 0.36 -1.8 2,728,935 - ABZR86_RS12205 0.36 -0.5 2,729,303 + ABZR86_RS12205 0.81 -1.5 2,729,304 - ABZR86_RS12205 0.82 -3.0 2,729,304 - ABZR86_RS12205 0.82 -2.3 2,729,482 + ABZR86_RS12210 0.11 -0.5 2,729,483 - ABZR86_RS12210 0.11 +0.5 2,729,593 + ABZR86_RS12210 0.46 -1.4 2,729,593 + ABZR86_RS12210 0.46 -0.7 2,729,594 - ABZR86_RS12210 0.47 -0.2 2,729,594 - ABZR86_RS12210 0.47 +0.1 2,729,594 - ABZR86_RS12210 0.47 -0.6 2,729,737 - -1.2 2,729,805 + -0.8 2,729,805 + +0.1 2,729,806 - -0.3 2,729,808 + -0.1 2,729,808 + +0.5 2,729,808 + +0.0 2,729,808 + +0.8 2,729,808 + +0.9 2,729,808 + +0.9 2,729,808 + -0.5 2,729,808 + +0.7 2,729,809 - +1.1 2,729,809 - +0.5 2,729,809 - +1.0 2,729,809 - +1.5 2,729,809 - +0.8 2,729,809 - +0.8 2,729,809 - +0.7 2,729,809 - +0.5 2,729,809 - +0.5 2,729,848 - +1.8 2,730,173 + ABZR86_RS12215 0.31 +0.1 2,730,267 - ABZR86_RS12215 0.42 -0.9
Or see this region's nucleotide sequence