Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS11110 and ABZR86_RS11115 are separated by 39 nucleotides ABZR86_RS11115 and ABZR86_RS11120 are separated by 349 nucleotides ABZR86_RS11120 and ABZR86_RS11125 overlap by 4 nucleotides
ABZR86_RS11110: ABZR86_RS11110 - AGE family epimerase/isomerase, at 2,477,111 to 2,478,325
_RS11110
ABZR86_RS11115: ABZR86_RS11115 - carboxymuconolactone decarboxylase family protein, at 2,478,365 to 2,478,823
_RS11115
ABZR86_RS11120: ABZR86_RS11120 - efflux transporter outer membrane subunit, at 2,479,173 to 2,480,609
_RS11120
ABZR86_RS11125: ABZR86_RS11125 - efflux RND transporter permease subunit, at 2,480,606 to 2,483,803
_RS11125
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-1
0
1
2 at 2478.186 kb on - strand, within ABZR86_RS11110 at 2478.209 kb on - strand at 2478.209 kb on - strand at 2478.209 kb on - strand at 2478.227 kb on + strand at 2478.227 kb on + strand at 2478.228 kb on - strand at 2478.263 kb on + strand at 2478.333 kb on + strand at 2478.333 kb on + strand at 2478.334 kb on - strand at 2478.367 kb on - strand at 2478.423 kb on + strand, within ABZR86_RS11115 at 2478.427 kb on + strand, within ABZR86_RS11115 at 2478.427 kb on + strand, within ABZR86_RS11115 at 2478.427 kb on + strand, within ABZR86_RS11115 at 2478.479 kb on + strand, within ABZR86_RS11115 at 2478.480 kb on - strand, within ABZR86_RS11115 at 2478.498 kb on + strand, within ABZR86_RS11115 at 2478.498 kb on + strand, within ABZR86_RS11115 at 2478.498 kb on + strand, within ABZR86_RS11115 at 2478.499 kb on - strand, within ABZR86_RS11115 at 2478.665 kb on - strand, within ABZR86_RS11115 at 2478.665 kb on - strand, within ABZR86_RS11115 at 2478.675 kb on + strand, within ABZR86_RS11115 at 2478.675 kb on + strand, within ABZR86_RS11115 at 2478.753 kb on + strand, within ABZR86_RS11115 at 2478.753 kb on + strand, within ABZR86_RS11115 at 2478.753 kb on + strand, within ABZR86_RS11115 at 2478.753 kb on + strand, within ABZR86_RS11115 at 2478.804 kb on + strand at 2478.824 kb on - strand at 2478.824 kb on - strand at 2478.824 kb on - strand at 2478.863 kb on + strand at 2478.863 kb on + strand at 2479.043 kb on + strand at 2479.049 kb on + strand at 2479.049 kb on + strand at 2479.050 kb on - strand at 2479.050 kb on - strand at 2479.051 kb on + strand at 2479.052 kb on - strand at 2479.052 kb on - strand at 2479.091 kb on - strand at 2479.167 kb on + strand at 2479.168 kb on - strand at 2479.168 kb on - strand at 2479.168 kb on - strand at 2479.209 kb on + strand at 2479.483 kb on + strand, within ABZR86_RS11120 at 2479.483 kb on + strand, within ABZR86_RS11120 at 2479.484 kb on - strand, within ABZR86_RS11120 at 2479.815 kb on - strand, within ABZR86_RS11120 at 2480.093 kb on - strand, within ABZR86_RS11120 at 2480.164 kb on + strand, within ABZR86_RS11120 at 2480.165 kb on - strand, within ABZR86_RS11120 at 2480.188 kb on + strand, within ABZR86_RS11120 at 2480.189 kb on - strand, within ABZR86_RS11120 at 2480.198 kb on - strand, within ABZR86_RS11120 at 2480.198 kb on - strand, within ABZR86_RS11120 at 2480.202 kb on - strand, within ABZR86_RS11120 at 2480.202 kb on - strand, within ABZR86_RS11120 at 2480.504 kb on - strand at 2480.649 kb on + strand at 2480.661 kb on + strand at 2480.677 kb on - strand at 2481.294 kb on + strand, within ABZR86_RS11125 at 2481.307 kb on - strand, within ABZR86_RS11125
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 2,478,186 - ABZR86_RS11110 0.88 +0.6 2,478,209 - +0.5 2,478,209 - -0.7 2,478,209 - +0.1 2,478,227 + +1.7 2,478,227 + +1.1 2,478,228 - +0.2 2,478,263 + +0.1 2,478,333 + +0.6 2,478,333 + +1.2 2,478,334 - +0.0 2,478,367 - +0.5 2,478,423 + ABZR86_RS11115 0.13 +0.2 2,478,427 + ABZR86_RS11115 0.14 -0.7 2,478,427 + ABZR86_RS11115 0.14 -1.0 2,478,427 + ABZR86_RS11115 0.14 -1.0 2,478,479 + ABZR86_RS11115 0.25 +1.6 2,478,480 - ABZR86_RS11115 0.25 +0.8 2,478,498 + ABZR86_RS11115 0.29 -0.2 2,478,498 + ABZR86_RS11115 0.29 -0.5 2,478,498 + ABZR86_RS11115 0.29 -1.0 2,478,499 - ABZR86_RS11115 0.29 +1.1 2,478,665 - ABZR86_RS11115 0.65 +1.5 2,478,665 - ABZR86_RS11115 0.65 +0.3 2,478,675 + ABZR86_RS11115 0.68 +0.1 2,478,675 + ABZR86_RS11115 0.68 -1.5 2,478,753 + ABZR86_RS11115 0.85 -0.1 2,478,753 + ABZR86_RS11115 0.85 -0.2 2,478,753 + ABZR86_RS11115 0.85 +0.2 2,478,753 + ABZR86_RS11115 0.85 +0.1 2,478,804 + +0.3 2,478,824 - +0.3 2,478,824 - +0.2 2,478,824 - -0.6 2,478,863 + +0.6 2,478,863 + -0.0 2,479,043 + -0.0 2,479,049 + -0.5 2,479,049 + +0.8 2,479,050 - -0.4 2,479,050 - +0.4 2,479,051 + -0.3 2,479,052 - -0.1 2,479,052 - +0.4 2,479,091 - -0.2 2,479,167 + +0.7 2,479,168 - +0.5 2,479,168 - -1.1 2,479,168 - +0.2 2,479,209 + +0.3 2,479,483 + ABZR86_RS11120 0.22 +0.2 2,479,483 + ABZR86_RS11120 0.22 -0.9 2,479,484 - ABZR86_RS11120 0.22 -0.1 2,479,815 - ABZR86_RS11120 0.45 +0.3 2,480,093 - ABZR86_RS11120 0.64 +1.2 2,480,164 + ABZR86_RS11120 0.69 +2.4 2,480,165 - ABZR86_RS11120 0.69 +0.5 2,480,188 + ABZR86_RS11120 0.71 +0.1 2,480,189 - ABZR86_RS11120 0.71 +0.7 2,480,198 - ABZR86_RS11120 0.71 -0.3 2,480,198 - ABZR86_RS11120 0.71 +0.3 2,480,202 - ABZR86_RS11120 0.72 +0.6 2,480,202 - ABZR86_RS11120 0.72 +0.4 2,480,504 - +0.4 2,480,649 + -0.0 2,480,661 + +2.2 2,480,677 - +0.2 2,481,294 + ABZR86_RS11125 0.22 -0.4 2,481,307 - ABZR86_RS11125 0.22 +0.5
Or see this region's nucleotide sequence