Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10785 and ABZR86_RS10790 are separated by 110 nucleotides ABZR86_RS10790 and ABZR86_RS10795 are separated by 10 nucleotides ABZR86_RS10795 and ABZR86_RS10800 are separated by 2 nucleotides ABZR86_RS10800 and ABZR86_RS10805 are separated by 244 nucleotides
ABZR86_RS10785: ABZR86_RS10785 - 7-cyano-7-deazaguanine synthase QueC, at 2,396,114 to 2,396,809
_RS10785
ABZR86_RS10790: ABZR86_RS10790 - 7-carboxy-7-deazaguanine synthase QueE, at 2,396,920 to 2,397,612
_RS10790
ABZR86_RS10795: ABZR86_RS10795 - tol-pal system protein YbgF, at 2,397,623 to 2,398,513
_RS10795
ABZR86_RS10800: ABZR86_RS10800 - peptidoglycan-associated lipoprotein Pal, at 2,398,516 to 2,399,025
_RS10800
ABZR86_RS10805: ABZR86_RS10805 - Tol-Pal system beta propeller repeat protein TolB, at 2,399,270 to 2,400,589
_RS10805
Position (kb)
2397
2398
2399 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2397.104 kb on + strand, within ABZR86_RS10790 at 2397.434 kb on + strand, within ABZR86_RS10790 at 2397.435 kb on - strand, within ABZR86_RS10790 at 2397.693 kb on + strand at 2397.693 kb on + strand at 2397.693 kb on + strand at 2397.693 kb on + strand at 2397.694 kb on - strand at 2397.846 kb on + strand, within ABZR86_RS10795 at 2397.847 kb on - strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.861 kb on + strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.862 kb on - strand, within ABZR86_RS10795 at 2397.864 kb on + strand, within ABZR86_RS10795 at 2397.864 kb on + strand, within ABZR86_RS10795 at 2397.864 kb on + strand, within ABZR86_RS10795 at 2397.864 kb on + strand, within ABZR86_RS10795 at 2397.864 kb on + strand, within ABZR86_RS10795 at 2397.865 kb on - strand, within ABZR86_RS10795 at 2397.865 kb on - strand, within ABZR86_RS10795 at 2397.882 kb on + strand, within ABZR86_RS10795 at 2397.882 kb on + strand, within ABZR86_RS10795 at 2397.882 kb on + strand, within ABZR86_RS10795 at 2397.883 kb on - strand, within ABZR86_RS10795 at 2397.885 kb on + strand, within ABZR86_RS10795 at 2397.885 kb on + strand, within ABZR86_RS10795 at 2397.885 kb on + strand, within ABZR86_RS10795 at 2397.886 kb on - strand, within ABZR86_RS10795 at 2397.886 kb on - strand, within ABZR86_RS10795 at 2397.886 kb on - strand, within ABZR86_RS10795 at 2397.957 kb on + strand, within ABZR86_RS10795 at 2397.957 kb on + strand, within ABZR86_RS10795 at 2397.957 kb on + strand, within ABZR86_RS10795 at 2397.958 kb on - strand, within ABZR86_RS10795 at 2397.958 kb on - strand, within ABZR86_RS10795 at 2397.993 kb on + strand, within ABZR86_RS10795 at 2397.993 kb on + strand, within ABZR86_RS10795 at 2397.994 kb on - strand, within ABZR86_RS10795 at 2397.994 kb on - strand, within ABZR86_RS10795 at 2397.994 kb on - strand, within ABZR86_RS10795 at 2398.233 kb on + strand, within ABZR86_RS10795 at 2398.233 kb on + strand, within ABZR86_RS10795 at 2398.478 kb on + strand at 2398.478 kb on + strand at 2398.479 kb on - strand at 2398.517 kb on + strand at 2398.517 kb on + strand at 2398.517 kb on + strand at 2398.517 kb on + strand at 2398.517 kb on + strand at 2398.518 kb on - strand at 2398.518 kb on - strand at 2399.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 2,397,104 + ABZR86_RS10790 0.27 -4.7 2,397,434 + ABZR86_RS10790 0.74 -2.7 2,397,435 - ABZR86_RS10790 0.74 -0.5 2,397,693 + -3.2 2,397,693 + -1.8 2,397,693 + -0.2 2,397,693 + -3.4 2,397,694 - -0.9 2,397,846 + ABZR86_RS10795 0.25 -0.2 2,397,847 - ABZR86_RS10795 0.25 -0.0 2,397,861 + ABZR86_RS10795 0.27 -0.8 2,397,861 + ABZR86_RS10795 0.27 -1.1 2,397,861 + ABZR86_RS10795 0.27 -0.7 2,397,861 + ABZR86_RS10795 0.27 -1.9 2,397,861 + ABZR86_RS10795 0.27 -0.1 2,397,861 + ABZR86_RS10795 0.27 -2.1 2,397,862 - ABZR86_RS10795 0.27 -2.1 2,397,862 - ABZR86_RS10795 0.27 -1.0 2,397,862 - ABZR86_RS10795 0.27 +0.4 2,397,862 - ABZR86_RS10795 0.27 -1.9 2,397,862 - ABZR86_RS10795 0.27 -3.6 2,397,862 - ABZR86_RS10795 0.27 -1.8 2,397,864 + ABZR86_RS10795 0.27 -0.5 2,397,864 + ABZR86_RS10795 0.27 -1.1 2,397,864 + ABZR86_RS10795 0.27 -0.4 2,397,864 + ABZR86_RS10795 0.27 -2.2 2,397,864 + ABZR86_RS10795 0.27 -1.1 2,397,865 - ABZR86_RS10795 0.27 -0.6 2,397,865 - ABZR86_RS10795 0.27 -1.0 2,397,882 + ABZR86_RS10795 0.29 -1.2 2,397,882 + ABZR86_RS10795 0.29 -1.4 2,397,882 + ABZR86_RS10795 0.29 -3.2 2,397,883 - ABZR86_RS10795 0.29 +0.7 2,397,885 + ABZR86_RS10795 0.29 -0.2 2,397,885 + ABZR86_RS10795 0.29 -2.1 2,397,885 + ABZR86_RS10795 0.29 -0.8 2,397,886 - ABZR86_RS10795 0.30 -1.0 2,397,886 - ABZR86_RS10795 0.30 -0.2 2,397,886 - ABZR86_RS10795 0.30 -3.0 2,397,957 + ABZR86_RS10795 0.37 -2.1 2,397,957 + ABZR86_RS10795 0.37 -1.1 2,397,957 + ABZR86_RS10795 0.37 -0.9 2,397,958 - ABZR86_RS10795 0.38 -1.0 2,397,958 - ABZR86_RS10795 0.38 -0.2 2,397,993 + ABZR86_RS10795 0.42 -1.3 2,397,993 + ABZR86_RS10795 0.42 -0.8 2,397,994 - ABZR86_RS10795 0.42 -2.9 2,397,994 - ABZR86_RS10795 0.42 -1.3 2,397,994 - ABZR86_RS10795 0.42 -0.6 2,398,233 + ABZR86_RS10795 0.68 -0.7 2,398,233 + ABZR86_RS10795 0.68 -2.5 2,398,478 + -2.4 2,398,478 + -2.7 2,398,479 - -0.9 2,398,517 + -1.1 2,398,517 + -1.4 2,398,517 + -2.9 2,398,517 + -3.2 2,398,517 + -1.6 2,398,518 - +0.2 2,398,518 - -3.2 2,399,128 + -1.0
Or see this region's nucleotide sequence