Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10230 and ABZR86_RS10235 are separated by 9 nucleotides ABZR86_RS10235 and ABZR86_RS10240 are separated by 33 nucleotides ABZR86_RS10240 and ABZR86_RS10245 are separated by 38 nucleotides ABZR86_RS10245 and ABZR86_RS10250 are separated by 55 nucleotides
ABZR86_RS10230: ABZR86_RS10230 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 2,271,030 to 2,271,767
_RS10230
ABZR86_RS10235: ABZR86_RS10235 - DUF4279 domain-containing protein, at 2,271,777 to 2,272,175
_RS10235
ABZR86_RS10240: ABZR86_RS10240 - tetratricopeptide repeat protein, at 2,272,209 to 2,272,583
_RS10240
ABZR86_RS10245: ABZR86_RS10245 - hypothetical protein, at 2,272,622 to 2,272,960
_RS10245
ABZR86_RS10250: ABZR86_RS10250 - hypothetical protein, at 2,273,016 to 2,273,375
_RS10250
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2270.973 kb on - strand at 2271.005 kb on - strand at 2271.178 kb on - strand, within ABZR86_RS10230 at 2271.538 kb on + strand, within ABZR86_RS10230 at 2271.538 kb on + strand, within ABZR86_RS10230 at 2271.539 kb on - strand, within ABZR86_RS10230 at 2271.539 kb on - strand, within ABZR86_RS10230 at 2271.608 kb on - strand, within ABZR86_RS10230 at 2271.707 kb on + strand at 2271.796 kb on + strand at 2271.797 kb on - strand at 2271.797 kb on - strand at 2271.936 kb on - strand, within ABZR86_RS10235 at 2271.936 kb on - strand, within ABZR86_RS10235 at 2271.963 kb on + strand, within ABZR86_RS10235 at 2271.963 kb on + strand, within ABZR86_RS10235 at 2271.964 kb on - strand, within ABZR86_RS10235 at 2271.971 kb on + strand, within ABZR86_RS10235 at 2271.972 kb on - strand, within ABZR86_RS10235 at 2272.162 kb on + strand at 2272.162 kb on + strand at 2272.210 kb on + strand at 2272.210 kb on + strand at 2272.211 kb on - strand at 2272.213 kb on + strand at 2272.214 kb on - strand at 2272.310 kb on - strand, within ABZR86_RS10240 at 2272.325 kb on + strand, within ABZR86_RS10240 at 2272.325 kb on + strand, within ABZR86_RS10240 at 2272.328 kb on - strand, within ABZR86_RS10240 at 2272.328 kb on - strand, within ABZR86_RS10240 at 2272.367 kb on - strand, within ABZR86_RS10240 at 2272.372 kb on + strand, within ABZR86_RS10240 at 2272.417 kb on + strand, within ABZR86_RS10240 at 2272.418 kb on - strand, within ABZR86_RS10240 at 2272.568 kb on + strand at 2272.569 kb on - strand at 2272.604 kb on + strand at 2272.605 kb on - strand at 2272.737 kb on + strand, within ABZR86_RS10245 at 2272.799 kb on - strand, within ABZR86_RS10245 at 2272.826 kb on - strand, within ABZR86_RS10245 at 2272.833 kb on - strand, within ABZR86_RS10245 at 2272.833 kb on - strand, within ABZR86_RS10245 at 2272.891 kb on + strand, within ABZR86_RS10245 at 2272.892 kb on - strand, within ABZR86_RS10245 at 2273.047 kb on + strand at 2273.047 kb on + strand at 2273.047 kb on + strand at 2273.071 kb on + strand, within ABZR86_RS10250 at 2273.071 kb on + strand, within ABZR86_RS10250 at 2273.072 kb on - strand, within ABZR86_RS10250 at 2273.072 kb on - strand, within ABZR86_RS10250 at 2273.085 kb on + strand, within ABZR86_RS10250 at 2273.167 kb on + strand, within ABZR86_RS10250 at 2273.167 kb on + strand, within ABZR86_RS10250 at 2273.167 kb on + strand, within ABZR86_RS10250
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 2,270,973 - -0.1 2,271,005 - -0.0 2,271,178 - ABZR86_RS10230 0.20 +1.2 2,271,538 + ABZR86_RS10230 0.69 +1.3 2,271,538 + ABZR86_RS10230 0.69 -0.2 2,271,539 - ABZR86_RS10230 0.69 +0.7 2,271,539 - ABZR86_RS10230 0.69 +0.8 2,271,608 - ABZR86_RS10230 0.78 +1.1 2,271,707 + +1.5 2,271,796 + +0.2 2,271,797 - -0.2 2,271,797 - -0.4 2,271,936 - ABZR86_RS10235 0.40 +0.3 2,271,936 - ABZR86_RS10235 0.40 -0.5 2,271,963 + ABZR86_RS10235 0.47 +0.9 2,271,963 + ABZR86_RS10235 0.47 +0.9 2,271,964 - ABZR86_RS10235 0.47 +0.1 2,271,971 + ABZR86_RS10235 0.49 +0.2 2,271,972 - ABZR86_RS10235 0.49 +0.5 2,272,162 + -1.9 2,272,162 + +0.2 2,272,210 + +1.2 2,272,210 + +1.1 2,272,211 - +0.8 2,272,213 + -0.9 2,272,214 - -0.4 2,272,310 - ABZR86_RS10240 0.27 -1.1 2,272,325 + ABZR86_RS10240 0.31 +0.8 2,272,325 + ABZR86_RS10240 0.31 +0.3 2,272,328 - ABZR86_RS10240 0.32 +1.4 2,272,328 - ABZR86_RS10240 0.32 -0.6 2,272,367 - ABZR86_RS10240 0.42 +0.3 2,272,372 + ABZR86_RS10240 0.43 +0.9 2,272,417 + ABZR86_RS10240 0.55 -0.6 2,272,418 - ABZR86_RS10240 0.56 +0.2 2,272,568 + +0.4 2,272,569 - -0.3 2,272,604 + +0.8 2,272,605 - +0.4 2,272,737 + ABZR86_RS10245 0.34 +2.0 2,272,799 - ABZR86_RS10245 0.52 +0.6 2,272,826 - ABZR86_RS10245 0.60 +0.7 2,272,833 - ABZR86_RS10245 0.62 +0.1 2,272,833 - ABZR86_RS10245 0.62 +0.1 2,272,891 + ABZR86_RS10245 0.79 -0.2 2,272,892 - ABZR86_RS10245 0.80 -0.5 2,273,047 + +0.1 2,273,047 + +0.3 2,273,047 + -0.1 2,273,071 + ABZR86_RS10250 0.15 +0.1 2,273,071 + ABZR86_RS10250 0.15 -0.5 2,273,072 - ABZR86_RS10250 0.16 -0.4 2,273,072 - ABZR86_RS10250 0.16 -2.0 2,273,085 + ABZR86_RS10250 0.19 -0.4 2,273,167 + ABZR86_RS10250 0.42 -0.6 2,273,167 + ABZR86_RS10250 0.42 +0.3 2,273,167 + ABZR86_RS10250 0.42 +0.1
Or see this region's nucleotide sequence