Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08770 and ABZR86_RS08775 overlap by 4 nucleotides ABZR86_RS08775 and ABZR86_RS08780 overlap by 4 nucleotides ABZR86_RS08780 and ABZR86_RS08785 are separated by 57 nucleotides ABZR86_RS08785 and ABZR86_RS08790 overlap by 8 nucleotides
ABZR86_RS08770: ABZR86_RS08770 - squalene/phytoene synthase family protein, at 1,962,920 to 1,963,771
_RS08770
ABZR86_RS08775: ABZR86_RS08775 - HAD-IA family hydrolase, at 1,963,768 to 1,964,448
_RS08775
ABZR86_RS08780: ABZR86_RS08780 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,964,445 to 1,965,152
_RS08780
ABZR86_RS08785: ABZR86_RS08785 - YciI family protein, at 1,965,210 to 1,965,482
_RS08785
ABZR86_RS08790: ABZR86_RS08790 - TRZ/ATZ family hydrolase, at 1,965,475 to 1,966,815
_RS08790
Position (kb)
1964
1965
1966 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1963.735 kb on - strand at 1963.893 kb on - strand, within ABZR86_RS08775 at 1964.205 kb on - strand, within ABZR86_RS08775 at 1964.205 kb on - strand, within ABZR86_RS08775 at 1964.217 kb on - strand, within ABZR86_RS08775 at 1965.160 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.206 kb on + strand at 1965.206 kb on + strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.281 kb on + strand, within ABZR86_RS08785 at 1965.282 kb on - strand, within ABZR86_RS08785 at 1965.282 kb on - strand, within ABZR86_RS08785 at 1965.319 kb on + strand, within ABZR86_RS08785 at 1965.320 kb on - strand, within ABZR86_RS08785 at 1965.320 kb on - strand, within ABZR86_RS08785 at 1965.454 kb on + strand, within ABZR86_RS08785 at 1965.455 kb on - strand at 1965.455 kb on - strand at 1965.626 kb on + strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.782 kb on + strand, within ABZR86_RS08790 at 1965.783 kb on - strand, within ABZR86_RS08790
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 1,963,735 - +3.5 1,963,893 - ABZR86_RS08775 0.18 -0.8 1,964,205 - ABZR86_RS08775 0.64 -0.9 1,964,205 - ABZR86_RS08775 0.64 -2.0 1,964,217 - ABZR86_RS08775 0.66 -1.3 1,965,160 - -0.7 1,965,175 - -0.4 1,965,175 - +0.0 1,965,175 - +0.0 1,965,175 - -0.3 1,965,175 - +0.1 1,965,175 - +0.2 1,965,175 - +0.0 1,965,206 + +0.0 1,965,206 + +1.4 1,965,207 - +0.1 1,965,207 - +0.9 1,965,207 - +0.6 1,965,207 - -1.3 1,965,207 - -0.3 1,965,257 + ABZR86_RS08785 0.17 -0.1 1,965,257 + ABZR86_RS08785 0.17 -0.4 1,965,257 + ABZR86_RS08785 0.17 +0.6 1,965,258 - ABZR86_RS08785 0.18 +0.6 1,965,258 - ABZR86_RS08785 0.18 -0.4 1,965,258 - ABZR86_RS08785 0.18 +0.2 1,965,259 + ABZR86_RS08785 0.18 +0.3 1,965,259 + ABZR86_RS08785 0.18 +0.5 1,965,259 + ABZR86_RS08785 0.18 -0.4 1,965,259 + ABZR86_RS08785 0.18 -0.7 1,965,259 + ABZR86_RS08785 0.18 +0.6 1,965,259 + ABZR86_RS08785 0.18 -0.3 1,965,260 - ABZR86_RS08785 0.18 +0.1 1,965,260 - ABZR86_RS08785 0.18 +0.3 1,965,260 - ABZR86_RS08785 0.18 -0.4 1,965,260 - ABZR86_RS08785 0.18 +0.8 1,965,281 + ABZR86_RS08785 0.26 +0.2 1,965,282 - ABZR86_RS08785 0.26 +0.6 1,965,282 - ABZR86_RS08785 0.26 +0.3 1,965,319 + ABZR86_RS08785 0.40 -0.9 1,965,320 - ABZR86_RS08785 0.40 +0.5 1,965,320 - ABZR86_RS08785 0.40 +0.1 1,965,454 + ABZR86_RS08785 0.89 -0.3 1,965,455 - +1.3 1,965,455 - +0.5 1,965,626 + ABZR86_RS08790 0.11 -0.2 1,965,627 - ABZR86_RS08790 0.11 -0.1 1,965,627 - ABZR86_RS08790 0.11 +0.3 1,965,627 - ABZR86_RS08790 0.11 -0.6 1,965,627 - ABZR86_RS08790 0.11 +0.2 1,965,627 - ABZR86_RS08790 0.11 -0.3 1,965,627 - ABZR86_RS08790 0.11 -0.6 1,965,782 + ABZR86_RS08790 0.23 +0.3 1,965,783 - ABZR86_RS08790 0.23 +0.1
Or see this region's nucleotide sequence