Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS07590 and ABZR86_RS07595 are separated by 199 nucleotides ABZR86_RS07595 and ABZR86_RS07600 are separated by 126 nucleotides ABZR86_RS07600 and ABZR86_RS07605 are separated by 76 nucleotides ABZR86_RS07605 and ABZR86_RS07610 are separated by 19 nucleotides ABZR86_RS07610 and ABZR86_RS07615 overlap by 4 nucleotides
ABZR86_RS07590: ABZR86_RS07590 - methionine synthase, at 1,680,555 to 1,683,242
_RS07590
ABZR86_RS07595: ABZR86_RS07595 - lipid-A-disaccharide synthase N-terminal domain-containing protein, at 1,683,442 to 1,683,771
_RS07595
ABZR86_RS07600: ABZR86_RS07600 - tRNA-Val, at 1,683,898 to 1,683,972
_RS07600
ABZR86_RS07605: ABZR86_RS07605 - SURF1 family protein, at 1,684,049 to 1,684,822
_RS07605
ABZR86_RS07610: ABZR86_RS07610 - cytochrome o ubiquinol oxidase subunit IV, at 1,684,842 to 1,685,255
_RS07610
ABZR86_RS07615: ABZR86_RS07615 - cytochrome o ubiquinol oxidase subunit III, at 1,685,252 to 1,685,887
_RS07615
Position (kb) 1684
1685 Strain fitness (log2 ratio) -1
0
1
2
3 at 1683.072 kb on + strand at 1683.072 kb on + strand at 1683.072 kb on + strand at 1683.072 kb on + strand at 1683.073 kb on - strand at 1683.073 kb on - strand at 1683.099 kb on + strand at 1683.099 kb on + strand at 1683.099 kb on + strand at 1683.100 kb on - strand at 1683.124 kb on - strand at 1683.124 kb on - strand at 1683.165 kb on + strand at 1683.229 kb on - strand at 1683.229 kb on - strand at 1683.272 kb on + strand at 1683.273 kb on - strand at 1683.373 kb on - strand at 1683.581 kb on + strand, within ABZR86_RS07595 at 1683.805 kb on + strand at 1683.805 kb on + strand at 1683.806 kb on - strand at 1684.135 kb on - strand, within ABZR86_RS07605 at 1684.135 kb on - strand, within ABZR86_RS07605 at 1684.135 kb on - strand, within ABZR86_RS07605 at 1684.135 kb on - strand, within ABZR86_RS07605 at 1684.300 kb on - strand, within ABZR86_RS07605 at 1684.545 kb on + strand, within ABZR86_RS07605 at 1684.897 kb on + strand, within ABZR86_RS07610 at 1684.897 kb on + strand, within ABZR86_RS07610 at 1684.897 kb on + strand, within ABZR86_RS07610 at 1684.898 kb on - strand, within ABZR86_RS07610 at 1684.898 kb on - strand, within ABZR86_RS07610 at 1684.898 kb on - strand, within ABZR86_RS07610 at 1684.898 kb on - strand, within ABZR86_RS07610 at 1684.898 kb on - strand, within ABZR86_RS07610 at 1685.005 kb on + strand, within ABZR86_RS07610 at 1685.005 kb on + strand, within ABZR86_RS07610 at 1685.006 kb on - strand, within ABZR86_RS07610 at 1685.006 kb on - strand, within ABZR86_RS07610 at 1685.146 kb on + strand, within ABZR86_RS07610 at 1685.274 kb on + strand at 1685.518 kb on - strand, within ABZR86_RS07615 at 1685.622 kb on + strand, within ABZR86_RS07615 at 1685.623 kb on - strand, within ABZR86_RS07615 at 1685.623 kb on - strand, within ABZR86_RS07615 at 1685.623 kb on - strand, within ABZR86_RS07615 at 1685.653 kb on + strand, within ABZR86_RS07615 at 1685.653 kb on + strand, within ABZR86_RS07615 at 1685.710 kb on - strand, within ABZR86_RS07615 at 1685.710 kb on - strand, within ABZR86_RS07615 at 1685.710 kb on - strand, within ABZR86_RS07615 at 1685.710 kb on - strand, within ABZR86_RS07615 at 1685.776 kb on - strand, within ABZR86_RS07615
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 1,683,072 + -0.3 1,683,072 + -0.4 1,683,072 + +0.6 1,683,072 + +0.1 1,683,073 - -0.1 1,683,073 - +0.2 1,683,099 + +0.0 1,683,099 + -0.4 1,683,099 + -0.1 1,683,100 - -0.0 1,683,124 - +0.9 1,683,124 - -0.9 1,683,165 + -1.1 1,683,229 - -0.8 1,683,229 - -0.2 1,683,272 + -0.4 1,683,273 - +0.0 1,683,373 - -0.2 1,683,581 + ABZR86_RS07595 0.42 -1.0 1,683,805 + -0.8 1,683,805 + -1.3 1,683,806 - -1.1 1,684,135 - ABZR86_RS07605 0.11 +1.3 1,684,135 - ABZR86_RS07605 0.11 +0.4 1,684,135 - ABZR86_RS07605 0.11 +0.8 1,684,135 - ABZR86_RS07605 0.11 +0.8 1,684,300 - ABZR86_RS07605 0.32 +1.2 1,684,545 + ABZR86_RS07605 0.64 +1.3 1,684,897 + ABZR86_RS07610 0.13 +2.0 1,684,897 + ABZR86_RS07610 0.13 +2.7 1,684,897 + ABZR86_RS07610 0.13 +2.0 1,684,898 - ABZR86_RS07610 0.14 +2.3 1,684,898 - ABZR86_RS07610 0.14 +1.9 1,684,898 - ABZR86_RS07610 0.14 +2.6 1,684,898 - ABZR86_RS07610 0.14 +2.3 1,684,898 - ABZR86_RS07610 0.14 +1.5 1,685,005 + ABZR86_RS07610 0.39 +2.8 1,685,005 + ABZR86_RS07610 0.39 +2.2 1,685,006 - ABZR86_RS07610 0.40 +3.0 1,685,006 - ABZR86_RS07610 0.40 +2.4 1,685,146 + ABZR86_RS07610 0.73 +3.1 1,685,274 + +2.4 1,685,518 - ABZR86_RS07615 0.42 +1.2 1,685,622 + ABZR86_RS07615 0.58 +2.0 1,685,623 - ABZR86_RS07615 0.58 +2.3 1,685,623 - ABZR86_RS07615 0.58 +1.7 1,685,623 - ABZR86_RS07615 0.58 +2.9 1,685,653 + ABZR86_RS07615 0.63 +0.2 1,685,653 + ABZR86_RS07615 0.63 +1.1 1,685,710 - ABZR86_RS07615 0.72 +1.7 1,685,710 - ABZR86_RS07615 0.72 +1.8 1,685,710 - ABZR86_RS07615 0.72 +2.0 1,685,710 - ABZR86_RS07615 0.72 +2.1 1,685,776 - ABZR86_RS07615 0.82 +2.4
Or see this region's nucleotide sequence