Experiment: formate McAA B12 dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05355 and MMJJ_RS05360 are separated by 116 nucleotides MMJJ_RS05360 and MMJJ_RS05365 are separated by 90 nucleotides MMJJ_RS05365 and MMJJ_RS05370 are separated by 31 nucleotides MMJJ_RS05370 and MMJJ_RS05375 are separated by 72 nucleotides
MMJJ_RS05355: MMJJ_RS05355 - DUF2121 domain-containing protein, at 995,237 to 996,112
_RS05355
MMJJ_RS05360: MMJJ_RS05360 - nickel-responsive transcriptional regulator NikR, at 996,229 to 996,654
_RS05360
MMJJ_RS05365: MMJJ_RS05365 - DUF2798 domain-containing protein, at 996,745 to 996,972
_RS05365
MMJJ_RS05370: MMJJ_RS05370 - LysR family transcriptional regulator, at 997,004 to 997,846
_RS05370
MMJJ_RS05375: MMJJ_RS05375 - radical SAM protein, at 997,919 to 998,971
_RS05375
Position (kb) 997
998 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 996.059 kb on + strand at 996.070 kb on - strand at 996.071 kb on + strand at 996.124 kb on - strand at 996.138 kb on + strand at 996.146 kb on + strand at 996.187 kb on - strand at 996.190 kb on - strand at 996.196 kb on - strand at 996.196 kb on - strand at 996.196 kb on - strand at 996.196 kb on - strand at 996.207 kb on - strand at 996.208 kb on + strand at 996.226 kb on + strand at 996.226 kb on + strand at 996.249 kb on + strand at 996.249 kb on + strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.252 kb on - strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.253 kb on + strand at 996.255 kb on + strand at 996.255 kb on + strand at 996.311 kb on + strand, within MMJJ_RS05360 at 996.411 kb on - strand, within MMJJ_RS05360 at 996.440 kb on - strand, within MMJJ_RS05360 at 996.440 kb on - strand, within MMJJ_RS05360 at 996.453 kb on + strand, within MMJJ_RS05360 at 996.462 kb on + strand, within MMJJ_RS05360 at 996.525 kb on - strand, within MMJJ_RS05360 at 996.533 kb on - strand, within MMJJ_RS05360 at 996.533 kb on - strand, within MMJJ_RS05360 at 996.565 kb on + strand, within MMJJ_RS05360 at 996.585 kb on - strand, within MMJJ_RS05360 at 996.586 kb on + strand, within MMJJ_RS05360 at 996.602 kb on - strand, within MMJJ_RS05360 at 996.612 kb on + strand at 996.623 kb on - strand at 996.678 kb on + strand at 996.718 kb on - strand at 996.719 kb on + strand at 996.734 kb on - strand at 996.734 kb on - strand at 996.742 kb on - strand at 996.743 kb on + strand at 996.743 kb on + strand at 996.743 kb on + strand at 996.763 kb on - strand at 996.785 kb on - strand, within MMJJ_RS05365 at 996.786 kb on + strand, within MMJJ_RS05365 at 996.812 kb on - strand, within MMJJ_RS05365 at 996.812 kb on - strand, within MMJJ_RS05365 at 996.812 kb on - strand, within MMJJ_RS05365 at 996.812 kb on - strand, within MMJJ_RS05365 at 996.825 kb on + strand, within MMJJ_RS05365 at 996.837 kb on + strand, within MMJJ_RS05365 at 996.850 kb on + strand, within MMJJ_RS05365 at 996.900 kb on - strand, within MMJJ_RS05365 at 996.900 kb on - strand, within MMJJ_RS05365 at 996.900 kb on - strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.901 kb on + strand, within MMJJ_RS05365 at 996.903 kb on + strand, within MMJJ_RS05365 at 996.919 kb on - strand, within MMJJ_RS05365 at 996.920 kb on + strand at 996.947 kb on - strand, within MMJJ_RS05365 at 996.960 kb on + strand at 996.960 kb on + strand at 996.963 kb on - strand at 996.966 kb on - strand at 996.970 kb on - strand at 996.977 kb on + strand at 996.984 kb on + strand at 996.987 kb on + strand at 996.998 kb on + strand at 996.998 kb on + strand at 997.001 kb on - strand at 997.001 kb on - strand at 997.002 kb on + strand at 997.054 kb on + strand at 997.055 kb on - strand at 997.111 kb on - strand, within MMJJ_RS05370 at 997.112 kb on + strand, within MMJJ_RS05370 at 997.183 kb on - strand, within MMJJ_RS05370 at 997.195 kb on - strand, within MMJJ_RS05370 at 997.199 kb on + strand, within MMJJ_RS05370 at 997.258 kb on - strand, within MMJJ_RS05370 at 997.311 kb on - strand, within MMJJ_RS05370 at 997.312 kb on + strand, within MMJJ_RS05370 at 997.316 kb on + strand, within MMJJ_RS05370 at 997.316 kb on + strand, within MMJJ_RS05370 at 997.362 kb on + strand, within MMJJ_RS05370 at 997.526 kb on - strand, within MMJJ_RS05370 at 997.528 kb on - strand, within MMJJ_RS05370 at 997.544 kb on + strand, within MMJJ_RS05370 at 997.572 kb on - strand, within MMJJ_RS05370 at 997.580 kb on + strand, within MMJJ_RS05370 at 997.582 kb on + strand at 997.585 kb on + strand, within MMJJ_RS05370 at 997.589 kb on - strand, within MMJJ_RS05370 at 997.590 kb on + strand, within MMJJ_RS05370 at 997.595 kb on + strand, within MMJJ_RS05370 at 997.790 kb on + strand at 997.795 kb on - strand at 997.796 kb on + strand at 997.796 kb on + strand at 997.797 kb on - strand at 997.797 kb on - strand at 997.797 kb on - strand at 997.797 kb on - strand at 997.797 kb on - strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.798 kb on + strand at 997.802 kb on - strand at 997.802 kb on - strand at 997.803 kb on + strand at 997.803 kb on + strand at 997.803 kb on + strand at 997.804 kb on - strand at 997.820 kb on - strand at 997.820 kb on - strand at 997.820 kb on - strand at 997.821 kb on + strand at 997.836 kb on + strand at 997.859 kb on - strand at 997.873 kb on - strand at 997.873 kb on - strand at 997.873 kb on - strand at 997.874 kb on + strand at 997.874 kb on + strand at 997.877 kb on + strand at 997.880 kb on + strand at 997.886 kb on - strand at 997.894 kb on - strand at 997.895 kb on + strand at 997.961 kb on + strand at 997.961 kb on + strand at 997.988 kb on + strand at 997.993 kb on - strand at 997.994 kb on + strand at 998.017 kb on + strand at 998.030 kb on + strand, within MMJJ_RS05375 at 998.030 kb on + strand, within MMJJ_RS05375 at 998.047 kb on - strand, within MMJJ_RS05375 at 998.065 kb on - strand, within MMJJ_RS05375 at 998.065 kb on - strand, within MMJJ_RS05375 at 998.065 kb on - strand, within MMJJ_RS05375 at 998.134 kb on - strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.135 kb on + strand, within MMJJ_RS05375 at 998.139 kb on - strand, within MMJJ_RS05375 at 998.190 kb on + strand, within MMJJ_RS05375 at 998.205 kb on - strand, within MMJJ_RS05375 at 998.205 kb on - strand, within MMJJ_RS05375 at 998.213 kb on + strand, within MMJJ_RS05375 at 998.231 kb on - strand, within MMJJ_RS05375 at 998.232 kb on + strand, within MMJJ_RS05375 at 998.253 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.257 kb on + strand, within MMJJ_RS05375 at 998.300 kb on + strand, within MMJJ_RS05375 at 998.331 kb on - strand, within MMJJ_RS05375 at 998.431 kb on - strand, within MMJJ_RS05375 at 998.432 kb on + strand, within MMJJ_RS05375 at 998.432 kb on + strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.434 kb on - strand, within MMJJ_RS05375 at 998.436 kb on - strand, within MMJJ_RS05375 at 998.442 kb on - strand, within MMJJ_RS05375 at 998.469 kb on - strand, within MMJJ_RS05375 at 998.470 kb on + strand, within MMJJ_RS05375 at 998.482 kb on + strand, within MMJJ_RS05375 at 998.499 kb on - strand, within MMJJ_RS05375 at 998.500 kb on + strand, within MMJJ_RS05375 at 998.515 kb on - strand, within MMJJ_RS05375 at 998.516 kb on + strand, within MMJJ_RS05375 at 998.516 kb on + strand, within MMJJ_RS05375 at 998.585 kb on - strand, within MMJJ_RS05375 at 998.585 kb on - strand, within MMJJ_RS05375 at 998.588 kb on + strand, within MMJJ_RS05375 at 998.634 kb on - strand, within MMJJ_RS05375 at 998.701 kb on + strand, within MMJJ_RS05375 at 998.701 kb on + strand, within MMJJ_RS05375 at 998.704 kb on - strand, within MMJJ_RS05375 at 998.735 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.788 kb on + strand, within MMJJ_RS05375 at 998.789 kb on - strand at 998.790 kb on + strand, within MMJJ_RS05375
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA B12 dropout remove 996,059 + -0.3 996,070 - +0.6 996,071 + +0.2 996,124 - -0.1 996,138 + -0.3 996,146 + -0.3 996,187 - -3.1 996,190 - -0.5 996,196 - +0.3 996,196 - -2.6 996,196 - -1.8 996,196 - -2.5 996,207 - -1.4 996,208 + -0.9 996,226 + -0.3 996,226 + -0.9 996,249 + -1.4 996,249 + -2.8 996,252 - -1.2 996,252 - -1.2 996,252 - -1.2 996,252 - -3.1 996,252 - -0.6 996,252 - -3.9 996,252 - -1.1 996,253 + -2.8 996,253 + -1.0 996,253 + +1.5 996,253 + -1.2 996,253 + -0.8 996,253 + -2.5 996,253 + -2.8 996,255 + -2.4 996,255 + -1.6 996,311 + MMJJ_RS05360 0.19 -0.7 996,411 - MMJJ_RS05360 0.43 -3.0 996,440 - MMJJ_RS05360 0.50 -2.3 996,440 - MMJJ_RS05360 0.50 -2.2 996,453 + MMJJ_RS05360 0.53 -1.5 996,462 + MMJJ_RS05360 0.55 -2.8 996,525 - MMJJ_RS05360 0.69 -0.1 996,533 - MMJJ_RS05360 0.71 -2.5 996,533 - MMJJ_RS05360 0.71 -2.9 996,565 + MMJJ_RS05360 0.79 -0.6 996,585 - MMJJ_RS05360 0.84 -1.4 996,586 + MMJJ_RS05360 0.84 -2.8 996,602 - MMJJ_RS05360 0.88 -1.5 996,612 + -0.8 996,623 - -1.6 996,678 + -1.0 996,718 - +0.3 996,719 + -0.7 996,734 - +0.6 996,734 - -0.0 996,742 - +0.5 996,743 + +0.7 996,743 + -0.2 996,743 + -1.5 996,763 - +0.8 996,785 - MMJJ_RS05365 0.18 -0.6 996,786 + MMJJ_RS05365 0.18 -0.1 996,812 - MMJJ_RS05365 0.29 -1.8 996,812 - MMJJ_RS05365 0.29 -1.0 996,812 - MMJJ_RS05365 0.29 +0.0 996,812 - MMJJ_RS05365 0.29 +1.1 996,825 + MMJJ_RS05365 0.35 -2.4 996,837 + MMJJ_RS05365 0.40 +0.8 996,850 + MMJJ_RS05365 0.46 -0.1 996,900 - MMJJ_RS05365 0.68 +0.7 996,900 - MMJJ_RS05365 0.68 -0.2 996,900 - MMJJ_RS05365 0.68 +0.7 996,901 + MMJJ_RS05365 0.68 +1.3 996,901 + MMJJ_RS05365 0.68 +1.3 996,901 + MMJJ_RS05365 0.68 -0.0 996,901 + MMJJ_RS05365 0.68 -0.4 996,901 + MMJJ_RS05365 0.68 +0.4 996,901 + MMJJ_RS05365 0.68 -0.3 996,903 + MMJJ_RS05365 0.69 -3.2 996,919 - MMJJ_RS05365 0.76 -1.1 996,920 + -1.2 996,947 - MMJJ_RS05365 0.89 -0.5 996,960 + -0.7 996,960 + +0.3 996,963 - -0.8 996,966 - +0.1 996,970 - +0.4 996,977 + +1.0 996,984 + -0.2 996,987 + +0.6 996,998 + +0.4 996,998 + -1.0 997,001 - +0.1 997,001 - -0.8 997,002 + -1.5 997,054 + -0.9 997,055 - -1.7 997,111 - MMJJ_RS05370 0.13 -2.5 997,112 + MMJJ_RS05370 0.13 -1.5 997,183 - MMJJ_RS05370 0.21 -1.3 997,195 - MMJJ_RS05370 0.23 -1.4 997,199 + MMJJ_RS05370 0.23 -1.4 997,258 - MMJJ_RS05370 0.30 -2.3 997,311 - MMJJ_RS05370 0.36 +0.2 997,312 + MMJJ_RS05370 0.37 -0.9 997,316 + MMJJ_RS05370 0.37 -2.5 997,316 + MMJJ_RS05370 0.37 -1.2 997,362 + MMJJ_RS05370 0.42 -1.9 997,526 - MMJJ_RS05370 0.62 -1.5 997,528 - MMJJ_RS05370 0.62 -0.6 997,544 + MMJJ_RS05370 0.64 -0.9 997,572 - MMJJ_RS05370 0.67 -2.4 997,580 + MMJJ_RS05370 0.68 -0.3 997,582 + -2.0 997,585 + MMJJ_RS05370 0.69 -0.6 997,589 - MMJJ_RS05370 0.69 -2.2 997,590 + MMJJ_RS05370 0.70 -1.6 997,595 + MMJJ_RS05370 0.70 -2.5 997,790 + -1.5 997,795 - -3.1 997,796 + -0.5 997,796 + -1.4 997,797 - -2.1 997,797 - -3.6 997,797 - -1.0 997,797 - -2.1 997,797 - -1.3 997,798 + -1.4 997,798 + -0.8 997,798 + -1.3 997,798 + +0.5 997,798 + -1.1 997,798 + -1.1 997,798 + -1.0 997,798 + -0.7 997,802 - -0.5 997,802 - -2.3 997,803 + -0.5 997,803 + -0.3 997,803 + -1.6 997,804 - -2.2 997,820 - -2.5 997,820 - -2.3 997,820 - -0.4 997,821 + -0.6 997,836 + -1.1 997,859 - -3.0 997,873 - -0.6 997,873 - -1.0 997,873 - -1.2 997,874 + +1.0 997,874 + -0.8 997,877 + -0.2 997,880 + -1.1 997,886 - -1.2 997,894 - -2.4 997,895 + -0.5 997,961 + -2.5 997,961 + -3.4 997,988 + -1.6 997,993 - -2.5 997,994 + -3.5 998,017 + -0.9 998,030 + MMJJ_RS05375 0.11 -0.8 998,030 + MMJJ_RS05375 0.11 -0.5 998,047 - MMJJ_RS05375 0.12 -1.9 998,065 - MMJJ_RS05375 0.14 -2.2 998,065 - MMJJ_RS05375 0.14 -1.7 998,065 - MMJJ_RS05375 0.14 -1.8 998,134 - MMJJ_RS05375 0.20 -2.8 998,135 + MMJJ_RS05375 0.21 -2.5 998,135 + MMJJ_RS05375 0.21 -2.4 998,135 + MMJJ_RS05375 0.21 -2.6 998,139 - MMJJ_RS05375 0.21 -0.1 998,190 + MMJJ_RS05375 0.26 -2.4 998,205 - MMJJ_RS05375 0.27 -2.4 998,205 - MMJJ_RS05375 0.27 -2.9 998,213 + MMJJ_RS05375 0.28 -2.0 998,231 - MMJJ_RS05375 0.30 -2.7 998,232 + MMJJ_RS05375 0.30 -4.2 998,253 + MMJJ_RS05375 0.32 -2.4 998,257 + MMJJ_RS05375 0.32 -1.5 998,257 + MMJJ_RS05375 0.32 -4.2 998,257 + MMJJ_RS05375 0.32 -0.8 998,257 + MMJJ_RS05375 0.32 -1.7 998,300 + MMJJ_RS05375 0.36 -4.1 998,331 - MMJJ_RS05375 0.39 -1.7 998,431 - MMJJ_RS05375 0.49 -1.7 998,432 + MMJJ_RS05375 0.49 -2.1 998,432 + MMJJ_RS05375 0.49 -1.7 998,434 - MMJJ_RS05375 0.49 -1.3 998,434 - MMJJ_RS05375 0.49 -2.6 998,434 - MMJJ_RS05375 0.49 -4.1 998,436 - MMJJ_RS05375 0.49 -1.0 998,442 - MMJJ_RS05375 0.50 -1.7 998,469 - MMJJ_RS05375 0.52 -1.3 998,470 + MMJJ_RS05375 0.52 -0.8 998,482 + MMJJ_RS05375 0.53 -1.5 998,499 - MMJJ_RS05375 0.55 -2.2 998,500 + MMJJ_RS05375 0.55 -3.0 998,515 - MMJJ_RS05375 0.57 -0.4 998,516 + MMJJ_RS05375 0.57 -1.6 998,516 + MMJJ_RS05375 0.57 -1.8 998,585 - MMJJ_RS05375 0.63 -2.1 998,585 - MMJJ_RS05375 0.63 -0.5 998,588 + MMJJ_RS05375 0.64 -1.9 998,634 - MMJJ_RS05375 0.68 -3.3 998,701 + MMJJ_RS05375 0.74 -2.9 998,701 + MMJJ_RS05375 0.74 -1.8 998,704 - MMJJ_RS05375 0.75 -2.0 998,735 + MMJJ_RS05375 0.77 -2.7 998,788 + MMJJ_RS05375 0.83 -2.8 998,788 + MMJJ_RS05375 0.83 -1.5 998,788 + MMJJ_RS05375 0.83 -2.0 998,789 - +0.8 998,790 + MMJJ_RS05375 0.83 -2.1
Or see this region's nucleotide sequence