Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05395 and H281DRAFT_05396 are separated by 454 nucleotides H281DRAFT_05396 and H281DRAFT_05397 are separated by 20 nucleotides
H281DRAFT_05395: H281DRAFT_05395 - Protein of unknown function (DUF3465), at 158,054 to 158,470
_05395
H281DRAFT_05396: H281DRAFT_05396 - lipoprotein NlpD, at 158,925 to 159,857
_05396
H281DRAFT_05397: H281DRAFT_05397 - Pimeloyl-ACP methyl ester carboxylesterase, at 159,878 to 160,705
_05397
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1 at 157.938 kb on - strand at 157.974 kb on - strand at 158.093 kb on + strand at 158.094 kb on - strand at 158.148 kb on + strand, within H281DRAFT_05395 at 158.148 kb on + strand, within H281DRAFT_05395 at 158.148 kb on + strand, within H281DRAFT_05395 at 158.191 kb on + strand, within H281DRAFT_05395 at 158.191 kb on + strand, within H281DRAFT_05395 at 158.192 kb on - strand, within H281DRAFT_05395 at 158.192 kb on - strand, within H281DRAFT_05395 at 158.351 kb on + strand, within H281DRAFT_05395 at 158.352 kb on - strand, within H281DRAFT_05395 at 158.352 kb on - strand, within H281DRAFT_05395 at 158.373 kb on - strand, within H281DRAFT_05395 at 158.439 kb on - strand at 158.448 kb on - strand at 158.549 kb on + strand at 158.616 kb on - strand at 158.668 kb on - strand at 158.686 kb on + strand at 158.687 kb on - strand at 158.908 kb on + strand at 158.908 kb on + strand at 158.908 kb on + strand at 158.908 kb on + strand at 158.909 kb on - strand at 159.157 kb on - strand, within H281DRAFT_05396 at 159.348 kb on - strand, within H281DRAFT_05396 at 159.556 kb on + strand, within H281DRAFT_05396 at 159.616 kb on - strand, within H281DRAFT_05396 at 159.616 kb on - strand, within H281DRAFT_05396 at 159.687 kb on + strand, within H281DRAFT_05396 at 160.101 kb on + strand, within H281DRAFT_05397 at 160.174 kb on - strand, within H281DRAFT_05397 at 160.224 kb on + strand, within H281DRAFT_05397 at 160.225 kb on - strand, within H281DRAFT_05397 at 160.591 kb on - strand, within H281DRAFT_05397 at 160.591 kb on - strand, within H281DRAFT_05397 at 160.591 kb on - strand, within H281DRAFT_05397 at 160.591 kb on - strand, within H281DRAFT_05397 at 160.614 kb on - strand, within H281DRAFT_05397 at 160.614 kb on - strand, within H281DRAFT_05397 at 160.676 kb on - strand at 160.676 kb on - strand at 160.677 kb on + strand at 160.678 kb on - strand at 160.783 kb on + strand at 160.784 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 157,938 - -0.3 157,974 - +0.9 158,093 + +0.1 158,094 - +0.7 158,148 + H281DRAFT_05395 0.23 -0.2 158,148 + H281DRAFT_05395 0.23 +0.1 158,148 + H281DRAFT_05395 0.23 +0.4 158,191 + H281DRAFT_05395 0.33 -1.0 158,191 + H281DRAFT_05395 0.33 -0.3 158,192 - H281DRAFT_05395 0.33 -1.0 158,192 - H281DRAFT_05395 0.33 -1.9 158,351 + H281DRAFT_05395 0.71 -0.0 158,352 - H281DRAFT_05395 0.71 +0.4 158,352 - H281DRAFT_05395 0.71 +0.2 158,373 - H281DRAFT_05395 0.76 -0.2 158,439 - +0.8 158,448 - +0.5 158,549 + -0.6 158,616 - +1.4 158,668 - +0.8 158,686 + -0.2 158,687 - -0.1 158,908 + +0.1 158,908 + -0.7 158,908 + -0.4 158,908 + -0.6 158,909 - +0.0 159,157 - H281DRAFT_05396 0.25 -0.5 159,348 - H281DRAFT_05396 0.45 +1.3 159,556 + H281DRAFT_05396 0.68 -0.4 159,616 - H281DRAFT_05396 0.74 +0.1 159,616 - H281DRAFT_05396 0.74 +0.5 159,687 + H281DRAFT_05396 0.82 +0.1 160,101 + H281DRAFT_05397 0.27 -0.3 160,174 - H281DRAFT_05397 0.36 +0.2 160,224 + H281DRAFT_05397 0.42 -0.4 160,225 - H281DRAFT_05397 0.42 +0.5 160,591 - H281DRAFT_05397 0.86 -1.6 160,591 - H281DRAFT_05397 0.86 -0.6 160,591 - H281DRAFT_05397 0.86 -0.9 160,591 - H281DRAFT_05397 0.86 -0.4 160,614 - H281DRAFT_05397 0.89 -0.3 160,614 - H281DRAFT_05397 0.89 -0.8 160,676 - +0.3 160,676 - +0.1 160,677 + -0.8 160,678 - -0.7 160,783 + +0.8 160,784 - +1.2
Or see this region's nucleotide sequence