Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05001 and H281DRAFT_05002 overlap by 16 nucleotides H281DRAFT_05002 and H281DRAFT_05003 overlap by 4 nucleotides H281DRAFT_05003 and H281DRAFT_05004 overlap by 4 nucleotides H281DRAFT_05004 and H281DRAFT_05005 are separated by 54 nucleotides
H281DRAFT_05001: H281DRAFT_05001 - type III secretion protein L, at 187,273 to 188,052
_05001
H281DRAFT_05002: H281DRAFT_05002 - type III secretion protein (HrpB4), at 188,037 to 188,852
_05002
H281DRAFT_05003: H281DRAFT_05003 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 188,849 to 189,670
_05003
H281DRAFT_05004: H281DRAFT_05004 - type III secretion inner rod protein HrpB2, at 189,667 to 190,059
_05004
H281DRAFT_05005: H281DRAFT_05005 - type III secretion protein HrpB1, at 190,114 to 190,731
_05005
Position (kb) 188
189
190 Strain fitness (log2 ratio) -2
-1
0
1 at 188.249 kb on + strand, within H281DRAFT_05002 at 188.689 kb on + strand, within H281DRAFT_05002 at 188.690 kb on - strand, within H281DRAFT_05002 at 188.694 kb on - strand, within H281DRAFT_05002 at 188.833 kb on + strand at 188.834 kb on - strand at 189.103 kb on + strand, within H281DRAFT_05003 at 189.104 kb on - strand, within H281DRAFT_05003 at 190.585 kb on - strand, within H281DRAFT_05005
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 188,249 + H281DRAFT_05002 0.26 -0.1 188,689 + H281DRAFT_05002 0.80 -0.7 188,690 - H281DRAFT_05002 0.80 -1.1 188,694 - H281DRAFT_05002 0.81 +0.1 188,833 + -0.6 188,834 - +0.5 189,103 + H281DRAFT_05003 0.31 -0.4 189,104 - H281DRAFT_05003 0.31 -2.1 190,585 - H281DRAFT_05005 0.76 -0.1
Or see this region's nucleotide sequence