Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04820 and H281DRAFT_04821 are separated by 203 nucleotides H281DRAFT_04821 and H281DRAFT_04822 are separated by 396 nucleotides
H281DRAFT_04820: H281DRAFT_04820 - Bis(5'nucleosyl)-tetraphosphatase, ApaH, at 254,819 to 255,703
_04820
H281DRAFT_04821: H281DRAFT_04821 - Nucleoside-diphosphate-sugar epimerase, at 255,907 to 256,818
_04821
H281DRAFT_04822: H281DRAFT_04822 - dTDP-glucose 4,6-dehydratase, at 257,215 to 258,276
_04822
Position (kb)
255
256
257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 255.177 kb on - strand, within H281DRAFT_04820 at 255.195 kb on + strand, within H281DRAFT_04820 at 255.328 kb on - strand, within H281DRAFT_04820 at 255.784 kb on + strand at 255.784 kb on + strand at 255.784 kb on + strand at 255.784 kb on + strand at 255.784 kb on + strand at 255.784 kb on + strand at 255.787 kb on + strand at 255.787 kb on + strand at 255.787 kb on + strand at 255.788 kb on - strand at 255.976 kb on + strand at 256.124 kb on - strand, within H281DRAFT_04821 at 256.124 kb on - strand, within H281DRAFT_04821 at 256.124 kb on - strand, within H281DRAFT_04821 at 256.124 kb on - strand, within H281DRAFT_04821 at 256.124 kb on - strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.125 kb on + strand, within H281DRAFT_04821 at 256.126 kb on - strand, within H281DRAFT_04821 at 256.239 kb on + strand, within H281DRAFT_04821 at 256.239 kb on + strand, within H281DRAFT_04821 at 256.240 kb on - strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.280 kb on - strand, within H281DRAFT_04821 at 256.280 kb on - strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.340 kb on - strand, within H281DRAFT_04821 at 256.423 kb on + strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.497 kb on + strand, within H281DRAFT_04821 at 256.513 kb on - strand, within H281DRAFT_04821 at 256.547 kb on - strand, within H281DRAFT_04821 at 256.547 kb on - strand, within H281DRAFT_04821 at 256.562 kb on - strand, within H281DRAFT_04821 at 256.562 kb on - strand, within H281DRAFT_04821 at 256.626 kb on + strand, within H281DRAFT_04821 at 256.626 kb on + strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.776 kb on + strand at 256.792 kb on - strand at 256.913 kb on - strand at 256.931 kb on + strand at 256.931 kb on + strand at 256.932 kb on - strand at 256.933 kb on + strand at 256.933 kb on + strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.940 kb on + strand at 256.941 kb on - strand at 256.941 kb on - strand at 256.942 kb on + strand at 256.942 kb on + strand at 256.943 kb on - strand at 256.943 kb on - strand at 256.946 kb on + strand at 256.946 kb on + strand at 257.038 kb on + strand at 257.047 kb on - strand at 257.071 kb on - strand at 257.071 kb on - strand at 257.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 255,177 - H281DRAFT_04820 0.40 +0.7 255,195 + H281DRAFT_04820 0.42 +0.8 255,328 - H281DRAFT_04820 0.58 -0.4 255,784 + -0.5 255,784 + -0.6 255,784 + +0.1 255,784 + -0.1 255,784 + -1.5 255,784 + +0.4 255,787 + +0.3 255,787 + -0.1 255,787 + -0.2 255,788 - +0.0 255,976 + +0.0 256,124 - H281DRAFT_04821 0.24 -0.4 256,124 - H281DRAFT_04821 0.24 -0.4 256,124 - H281DRAFT_04821 0.24 +0.3 256,124 - H281DRAFT_04821 0.24 +0.4 256,124 - H281DRAFT_04821 0.24 -1.2 256,125 + H281DRAFT_04821 0.24 -0.2 256,125 + H281DRAFT_04821 0.24 -0.6 256,125 + H281DRAFT_04821 0.24 +0.5 256,125 + H281DRAFT_04821 0.24 -0.3 256,125 + H281DRAFT_04821 0.24 +0.5 256,125 + H281DRAFT_04821 0.24 -0.3 256,126 - H281DRAFT_04821 0.24 +0.6 256,239 + H281DRAFT_04821 0.36 +1.1 256,239 + H281DRAFT_04821 0.36 -0.7 256,240 - H281DRAFT_04821 0.37 +0.2 256,279 + H281DRAFT_04821 0.41 +0.6 256,279 + H281DRAFT_04821 0.41 +0.6 256,279 + H281DRAFT_04821 0.41 +0.1 256,279 + H281DRAFT_04821 0.41 -0.0 256,280 - H281DRAFT_04821 0.41 -1.0 256,280 - H281DRAFT_04821 0.41 -0.5 256,339 + H281DRAFT_04821 0.47 +0.0 256,339 + H281DRAFT_04821 0.47 -2.9 256,339 + H281DRAFT_04821 0.47 -0.2 256,340 - H281DRAFT_04821 0.47 +0.5 256,423 + H281DRAFT_04821 0.57 +0.2 256,424 - H281DRAFT_04821 0.57 +0.9 256,424 - H281DRAFT_04821 0.57 -0.1 256,424 - H281DRAFT_04821 0.57 -0.1 256,424 - H281DRAFT_04821 0.57 -0.8 256,497 + H281DRAFT_04821 0.65 -1.8 256,513 - H281DRAFT_04821 0.66 +0.0 256,547 - H281DRAFT_04821 0.70 +0.8 256,547 - H281DRAFT_04821 0.70 +0.9 256,562 - H281DRAFT_04821 0.72 -1.0 256,562 - H281DRAFT_04821 0.72 -0.1 256,626 + H281DRAFT_04821 0.79 +1.0 256,626 + H281DRAFT_04821 0.79 +0.4 256,627 - H281DRAFT_04821 0.79 -0.1 256,627 - H281DRAFT_04821 0.79 -0.6 256,627 - H281DRAFT_04821 0.79 -0.2 256,627 - H281DRAFT_04821 0.79 +0.3 256,776 + +2.3 256,792 - -0.4 256,913 - +0.4 256,931 + -0.2 256,931 + +1.5 256,932 - -0.2 256,933 + -0.2 256,933 + +0.5 256,934 - -0.5 256,934 - +0.3 256,934 - -0.1 256,934 - +0.1 256,940 + -0.6 256,941 - -0.4 256,941 - -0.4 256,942 + -0.2 256,942 + +0.8 256,943 - -0.8 256,943 - +0.1 256,946 + -0.0 256,946 + +1.1 257,038 + +1.0 257,047 - +0.5 257,071 - +1.0 257,071 - +4.0 257,085 + +0.1
Or see this region's nucleotide sequence