Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04187 and H281DRAFT_04188 are separated by 142 nucleotides H281DRAFT_04188 and H281DRAFT_04189 are separated by 391 nucleotides
H281DRAFT_04187: H281DRAFT_04187 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 305,519 to 306,409
_04187
H281DRAFT_04188: H281DRAFT_04188 - fructose-bisphosphate aldolase, at 306,552 to 307,616
_04188
H281DRAFT_04189: H281DRAFT_04189 - pyruvate kinase, at 308,008 to 309,444
_04189
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 305.911 kb on - strand, within H281DRAFT_04187 at 306.340 kb on - strand at 306.340 kb on - strand at 306.526 kb on - strand at 306.546 kb on + strand at 306.546 kb on + strand at 306.586 kb on + strand at 306.586 kb on + strand at 306.587 kb on - strand at 306.587 kb on - strand at 307.629 kb on + strand at 307.630 kb on - strand at 307.695 kb on - strand at 307.930 kb on - strand at 307.937 kb on + strand at 308.012 kb on + strand at 308.583 kb on - strand, within H281DRAFT_04189 at 308.583 kb on - strand, within H281DRAFT_04189 at 308.583 kb on - strand, within H281DRAFT_04189 at 308.583 kb on - strand, within H281DRAFT_04189 at 308.583 kb on - strand, within H281DRAFT_04189
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 305,911 - H281DRAFT_04187 0.44 -1.0 306,340 - -0.6 306,340 - -2.9 306,526 - +0.5 306,546 + -2.5 306,546 + -3.3 306,586 + -2.5 306,586 + -0.6 306,587 - -2.9 306,587 - -0.4 307,629 + -2.0 307,630 - -1.0 307,695 - -0.9 307,930 - -0.8 307,937 + -2.1 308,012 + -0.8 308,583 - H281DRAFT_04189 0.40 +1.3 308,583 - H281DRAFT_04189 0.40 +2.2 308,583 - H281DRAFT_04189 0.40 +1.4 308,583 - H281DRAFT_04189 0.40 +1.4 308,583 - H281DRAFT_04189 0.40 +0.4
Or see this region's nucleotide sequence