Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04114 and H281DRAFT_04115 are separated by 114 nucleotides H281DRAFT_04115 and H281DRAFT_04116 are separated by 5 nucleotides
H281DRAFT_04114: H281DRAFT_04114 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 224,779 to 225,537
_04114
H281DRAFT_04115: H281DRAFT_04115 - Regulator of sirC expression, contains transglutaminase-like and TPR domains, at 225,652 to 226,494
_04115
H281DRAFT_04116: H281DRAFT_04116 - putative peptidoglycan lipid II flippase, at 226,500 to 228,050
_04116
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.718 kb on + strand at 224.724 kb on + strand at 224.725 kb on - strand at 224.750 kb on + strand at 224.750 kb on + strand at 224.751 kb on - strand at 224.751 kb on - strand at 224.751 kb on - strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.754 kb on + strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.755 kb on - strand at 224.798 kb on + strand at 224.798 kb on + strand at 224.799 kb on - strand at 224.808 kb on + strand at 225.223 kb on + strand, within H281DRAFT_04114 at 225.224 kb on - strand, within H281DRAFT_04114 at 225.251 kb on - strand, within H281DRAFT_04114 at 225.445 kb on + strand, within H281DRAFT_04114 at 225.445 kb on + strand, within H281DRAFT_04114 at 225.535 kb on + strand at 225.536 kb on - strand at 225.536 kb on - strand at 225.610 kb on - strand at 225.653 kb on + strand at 225.654 kb on - strand at 225.666 kb on + strand at 225.666 kb on + strand at 225.779 kb on + strand, within H281DRAFT_04115 at 225.841 kb on + strand, within H281DRAFT_04115 at 225.841 kb on + strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.842 kb on - strand, within H281DRAFT_04115 at 225.843 kb on + strand at 225.843 kb on + strand, within H281DRAFT_04115 at 225.843 kb on + strand, within H281DRAFT_04115 at 225.844 kb on - strand, within H281DRAFT_04115 at 225.896 kb on + strand, within H281DRAFT_04115 at 225.896 kb on + strand, within H281DRAFT_04115 at 225.921 kb on + strand, within H281DRAFT_04115 at 225.921 kb on + strand, within H281DRAFT_04115 at 225.922 kb on - strand, within H281DRAFT_04115 at 226.163 kb on + strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.239 kb on - strand, within H281DRAFT_04115 at 226.241 kb on + strand, within H281DRAFT_04115 at 226.241 kb on + strand, within H281DRAFT_04115 at 226.475 kb on + strand at 226.523 kb on - strand at 226.528 kb on + strand at 226.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 224,718 + -0.2 224,724 + -0.5 224,725 - -0.8 224,750 + +0.8 224,750 + -0.4 224,751 - +0.3 224,751 - +1.0 224,751 - -0.3 224,754 + +1.3 224,754 + -0.3 224,754 + +1.3 224,754 + -1.2 224,754 + +1.3 224,755 - -0.0 224,755 - +0.3 224,755 - -1.0 224,755 - +0.1 224,755 - +0.1 224,755 - +0.4 224,755 - +0.0 224,798 + +1.1 224,798 + +0.0 224,799 - -0.5 224,808 + -0.6 225,223 + H281DRAFT_04114 0.58 +0.3 225,224 - H281DRAFT_04114 0.59 +1.3 225,251 - H281DRAFT_04114 0.62 +0.6 225,445 + H281DRAFT_04114 0.88 +1.6 225,445 + H281DRAFT_04114 0.88 -0.1 225,535 + +0.1 225,536 - -0.1 225,536 - -0.3 225,610 - +0.9 225,653 + +1.1 225,654 - +0.0 225,666 + +0.3 225,666 + -1.9 225,779 + H281DRAFT_04115 0.15 -0.2 225,841 + H281DRAFT_04115 0.22 -0.2 225,841 + H281DRAFT_04115 0.22 +0.7 225,842 - H281DRAFT_04115 0.23 -0.8 225,842 - H281DRAFT_04115 0.23 -0.4 225,842 - H281DRAFT_04115 0.23 -0.7 225,843 + +2.0 225,843 + H281DRAFT_04115 0.23 -1.0 225,843 + H281DRAFT_04115 0.23 -1.2 225,844 - H281DRAFT_04115 0.23 -0.5 225,896 + H281DRAFT_04115 0.29 -0.6 225,896 + H281DRAFT_04115 0.29 -0.7 225,921 + H281DRAFT_04115 0.32 +1.0 225,921 + H281DRAFT_04115 0.32 +0.6 225,922 - H281DRAFT_04115 0.32 +0.4 226,163 + H281DRAFT_04115 0.61 -1.0 226,239 - H281DRAFT_04115 0.70 +0.8 226,239 - H281DRAFT_04115 0.70 +0.1 226,239 - H281DRAFT_04115 0.70 -0.6 226,239 - H281DRAFT_04115 0.70 -0.2 226,239 - H281DRAFT_04115 0.70 +0.1 226,241 + H281DRAFT_04115 0.70 +0.9 226,241 + H281DRAFT_04115 0.70 -0.4 226,475 + -0.3 226,523 - +0.5 226,528 + -1.7 226,528 + -0.2
Or see this region's nucleotide sequence