Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03515 and H281DRAFT_03516 are separated by 37 nucleotides H281DRAFT_03516 and H281DRAFT_03517 are separated by 153 nucleotides H281DRAFT_03517 and H281DRAFT_03518 are separated by 197 nucleotides H281DRAFT_03518 and H281DRAFT_03519 are separated by 439 nucleotides
H281DRAFT_03515: H281DRAFT_03515 - polyhydroxyalkanoate depolymerase, intracellular, at 15,272 to 16,735
_03515
H281DRAFT_03516: H281DRAFT_03516 - NADH-FMN oxidoreductase RutF, flavin reductase (DIM6/NTAB) family, at 16,773 to 17,384
_03516
H281DRAFT_03517: H281DRAFT_03517 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 17,538 to 18,413
_03517
H281DRAFT_03518: H281DRAFT_03518 - hypothetical protein, at 18,611 to 18,916
_03518
H281DRAFT_03519: H281DRAFT_03519 - hypothetical protein, at 19,356 to 19,514
_03519
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 16.645 kb on - strand at 16.888 kb on + strand, within H281DRAFT_03516 at 16.888 kb on + strand, within H281DRAFT_03516 at 16.889 kb on - strand, within H281DRAFT_03516 at 16.933 kb on + strand, within H281DRAFT_03516 at 16.933 kb on + strand, within H281DRAFT_03516 at 16.934 kb on - strand, within H281DRAFT_03516 at 16.934 kb on - strand, within H281DRAFT_03516 at 16.961 kb on + strand, within H281DRAFT_03516 at 16.962 kb on - strand, within H281DRAFT_03516 at 17.251 kb on + strand, within H281DRAFT_03516 at 17.368 kb on + strand at 17.414 kb on - strand at 17.476 kb on - strand at 17.540 kb on - strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.605 kb on + strand at 17.606 kb on - strand at 18.102 kb on - strand, within H281DRAFT_03517 at 18.102 kb on - strand, within H281DRAFT_03517 at 18.116 kb on + strand, within H281DRAFT_03517 at 18.116 kb on + strand, within H281DRAFT_03517 at 18.151 kb on + strand, within H281DRAFT_03517 at 18.435 kb on + strand at 18.436 kb on - strand at 18.436 kb on - strand at 18.438 kb on + strand at 18.438 kb on + strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.439 kb on - strand at 18.495 kb on - strand at 18.509 kb on + strand at 18.612 kb on + strand at 18.613 kb on - strand at 18.613 kb on - strand at 18.663 kb on + strand, within H281DRAFT_03518 at 18.663 kb on + strand, within H281DRAFT_03518 at 18.720 kb on + strand, within H281DRAFT_03518 at 18.724 kb on - strand, within H281DRAFT_03518 at 18.787 kb on - strand, within H281DRAFT_03518 at 18.916 kb on + strand at 18.916 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 19.090 kb on - strand at 19.172 kb on - strand at 19.193 kb on + strand at 19.224 kb on + strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.227 kb on - strand at 19.354 kb on + strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.357 kb on + strand at 19.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 16,645 - +1.6 16,888 + H281DRAFT_03516 0.19 -0.0 16,888 + H281DRAFT_03516 0.19 -0.7 16,889 - H281DRAFT_03516 0.19 -0.3 16,933 + H281DRAFT_03516 0.26 -0.3 16,933 + H281DRAFT_03516 0.26 -1.1 16,934 - H281DRAFT_03516 0.26 +0.1 16,934 - H281DRAFT_03516 0.26 -0.2 16,961 + H281DRAFT_03516 0.31 +0.3 16,962 - H281DRAFT_03516 0.31 +0.3 17,251 + H281DRAFT_03516 0.78 +0.1 17,368 + -0.3 17,414 - -0.4 17,476 - -1.2 17,540 - +0.7 17,605 + +0.3 17,605 + +0.1 17,605 + -0.0 17,605 + +1.2 17,605 + +0.2 17,606 - -0.1 18,102 - H281DRAFT_03517 0.64 -0.3 18,102 - H281DRAFT_03517 0.64 -0.2 18,116 + H281DRAFT_03517 0.66 +0.3 18,116 + H281DRAFT_03517 0.66 -0.2 18,151 + H281DRAFT_03517 0.70 +0.8 18,435 + +0.5 18,436 - -1.0 18,436 - -0.1 18,438 + -0.4 18,438 + -0.2 18,439 - +0.0 18,439 - -0.6 18,439 - +0.5 18,495 - +0.0 18,509 + +4.2 18,612 + -0.7 18,613 - -1.4 18,613 - -0.7 18,663 + H281DRAFT_03518 0.17 +1.5 18,663 + H281DRAFT_03518 0.17 +0.1 18,720 + H281DRAFT_03518 0.36 +0.4 18,724 - H281DRAFT_03518 0.37 +0.6 18,787 - H281DRAFT_03518 0.58 +0.4 18,916 + -0.0 18,916 + +0.6 18,916 + +0.1 18,917 - -0.3 18,917 - -1.0 18,917 - +1.0 18,917 - +0.4 19,090 - +0.2 19,172 - -0.4 19,193 + -0.1 19,224 + -0.1 19,225 - -0.3 19,225 - +0.0 19,225 - +0.3 19,226 + +0.2 19,226 + +0.2 19,226 + +0.2 19,227 - -0.8 19,354 + +0.1 19,355 - +0.7 19,355 - -0.4 19,355 - +0.6 19,355 - -1.7 19,357 + +0.5 19,357 + -0.1
Or see this region's nucleotide sequence