Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03410 and H281DRAFT_03411 are separated by 203 nucleotides H281DRAFT_03411 and H281DRAFT_03412 are separated by 99 nucleotides H281DRAFT_03412 and H281DRAFT_03413 are separated by 374 nucleotides
H281DRAFT_03410: H281DRAFT_03410 - Two-component sensor histidine kinase, contains HisKA and HATPase domains, at 480,155 to 481,546
_03410
H281DRAFT_03411: H281DRAFT_03411 - hypothetical protein, at 481,750 to 482,340
_03411
H281DRAFT_03412: H281DRAFT_03412 - Glycine-zipper containing OmpA-like membrane domain-containing protein, at 482,440 to 482,913
_03412
H281DRAFT_03413: H281DRAFT_03413 - high affinity sulphate transporter 1, at 483,288 to 485,081
_03413
Position (kb)
482
483 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 481.855 kb on + strand, within H281DRAFT_03411 at 482.067 kb on - strand, within H281DRAFT_03411 at 482.867 kb on - strand at 482.867 kb on - strand at 483.195 kb on + strand at 483.195 kb on + strand at 483.501 kb on + strand, within H281DRAFT_03413 at 483.501 kb on + strand, within H281DRAFT_03413 at 483.501 kb on + strand, within H281DRAFT_03413 at 483.502 kb on - strand, within H281DRAFT_03413 at 483.866 kb on + strand, within H281DRAFT_03413 at 483.870 kb on - strand, within H281DRAFT_03413 at 483.870 kb on - strand, within H281DRAFT_03413
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 481,855 + H281DRAFT_03411 0.18 -0.9 482,067 - H281DRAFT_03411 0.54 +0.3 482,867 - -0.6 482,867 - +0.0 483,195 + -1.2 483,195 + +0.3 483,501 + H281DRAFT_03413 0.12 -0.1 483,501 + H281DRAFT_03413 0.12 +4.0 483,501 + H281DRAFT_03413 0.12 -0.3 483,502 - H281DRAFT_03413 0.12 -0.1 483,866 + H281DRAFT_03413 0.32 -0.1 483,870 - H281DRAFT_03413 0.32 -0.7 483,870 - H281DRAFT_03413 0.32 -2.9
Or see this region's nucleotide sequence