Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02620 and H281DRAFT_02621 are separated by 107 nucleotides H281DRAFT_02621 and H281DRAFT_02622 overlap by 4 nucleotides H281DRAFT_02622 and H281DRAFT_02623 overlap by 20 nucleotides
H281DRAFT_02620: H281DRAFT_02620 - Sugar phosphate permease, at 196,426 to 197,742
_02620
H281DRAFT_02621: H281DRAFT_02621 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 197,850 to 199,193
_02621
H281DRAFT_02622: H281DRAFT_02622 - hypothetical protein, at 199,190 to 199,357
_02622
H281DRAFT_02623: H281DRAFT_02623 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 199,338 to 200,678
_02623
Position (kb) 197
198
199
200 Strain fitness (log2 ratio) -1
0
1 at 197.215 kb on - strand, within H281DRAFT_02620 at 197.314 kb on + strand, within H281DRAFT_02620 at 197.722 kb on + strand at 197.753 kb on - strand at 198.129 kb on + strand, within H281DRAFT_02621 at 198.129 kb on + strand, within H281DRAFT_02621 at 198.186 kb on + strand, within H281DRAFT_02621 at 198.187 kb on - strand, within H281DRAFT_02621 at 198.244 kb on - strand, within H281DRAFT_02621 at 198.759 kb on + strand, within H281DRAFT_02621 at 198.760 kb on - strand, within H281DRAFT_02621 at 198.760 kb on - strand, within H281DRAFT_02621 at 200.011 kb on - strand, within H281DRAFT_02623 at 200.177 kb on + strand, within H281DRAFT_02623
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 197,215 - H281DRAFT_02620 0.60 -0.7 197,314 + H281DRAFT_02620 0.67 -0.5 197,722 + +1.0 197,753 - -0.3 198,129 + H281DRAFT_02621 0.21 -0.5 198,129 + H281DRAFT_02621 0.21 +0.1 198,186 + H281DRAFT_02621 0.25 +1.2 198,187 - H281DRAFT_02621 0.25 +0.3 198,244 - H281DRAFT_02621 0.29 -0.8 198,759 + H281DRAFT_02621 0.68 -0.3 198,760 - H281DRAFT_02621 0.68 -1.6 198,760 - H281DRAFT_02621 0.68 +0.7 200,011 - H281DRAFT_02623 0.50 +0.7 200,177 + H281DRAFT_02623 0.63 -1.3
Or see this region's nucleotide sequence