Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01242

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01241 and H281DRAFT_01242 are separated by 255 nucleotidesH281DRAFT_01242 and H281DRAFT_01243 are separated by 57 nucleotidesH281DRAFT_01243 and H281DRAFT_01244 overlap by 4 nucleotides H281DRAFT_01241: H281DRAFT_01241 - mandelamide amidase, at 77,395 to 78,849 _01241 H281DRAFT_01242: H281DRAFT_01242 - CDP-diacylglycerol pyrophosphatase, at 79,105 to 79,746 _01242 H281DRAFT_01243: H281DRAFT_01243 - lipoprotein, YaeC family, at 79,804 to 80,616 _01243 H281DRAFT_01244: H281DRAFT_01244 - Formylglycine-generating enzyme, required for sulfatase activity, contains SUMF1/FGE domain, at 80,613 to 81,662 _01244 Position (kb) 79 80Strain fitness (log2 ratio) -2 -1 0 1 2at 78.517 kb on + strand, within H281DRAFT_01241at 78.517 kb on + strand, within H281DRAFT_01241at 78.648 kb on + strand, within H281DRAFT_01241at 78.673 kb on - strand, within H281DRAFT_01241at 78.818 kb on + strandat 78.875 kb on + strandat 79.213 kb on + strand, within H281DRAFT_01242at 79.213 kb on + strand, within H281DRAFT_01242at 79.744 kb on + strandat 79.940 kb on + strand, within H281DRAFT_01243at 80.303 kb on + strand, within H281DRAFT_01243at 80.595 kb on + strandat 80.652 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
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78,517 + H281DRAFT_01241 0.77 +0.8
78,517 + H281DRAFT_01241 0.77 -0.2
78,648 + H281DRAFT_01241 0.86 -2.2
78,673 - H281DRAFT_01241 0.88 +0.4
78,818 + +0.1
78,875 + -0.5
79,213 + H281DRAFT_01242 0.17 +1.0
79,213 + H281DRAFT_01242 0.17 +2.1
79,744 + +0.6
79,940 + H281DRAFT_01243 0.17 +0.9
80,303 + H281DRAFT_01243 0.61 +0.8
80,595 + +0.1
80,652 - +0.4

Or see this region's nucleotide sequence