Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00309 and H281DRAFT_00310 overlap by 46 nucleotides H281DRAFT_00310 and H281DRAFT_00311 are separated by 362 nucleotides
H281DRAFT_00309: H281DRAFT_00309 - glutamyl-Q tRNA(Asp) synthetase, at 334,012 to 334,974
_00309
H281DRAFT_00310: H281DRAFT_00310 - UPF0176 protein, at 334,929 to 335,792
_00310
H281DRAFT_00311: H281DRAFT_00311 - DNA polymerase III, alpha subunit, at 336,155 to 339,745
_00311
Position (kb)
334
335
336 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 333.938 kb on + strand at 334.209 kb on - strand, within H281DRAFT_00309 at 334.238 kb on + strand, within H281DRAFT_00309 at 334.351 kb on + strand, within H281DRAFT_00309 at 334.352 kb on - strand, within H281DRAFT_00309 at 334.367 kb on + strand, within H281DRAFT_00309 at 334.367 kb on + strand, within H281DRAFT_00309 at 334.368 kb on - strand, within H281DRAFT_00309 at 334.569 kb on + strand, within H281DRAFT_00309 at 334.570 kb on - strand, within H281DRAFT_00309 at 334.710 kb on - strand, within H281DRAFT_00309 at 334.710 kb on - strand, within H281DRAFT_00309 at 334.710 kb on - strand, within H281DRAFT_00309 at 334.710 kb on - strand, within H281DRAFT_00309 at 334.990 kb on + strand at 334.990 kb on + strand at 334.991 kb on - strand at 334.991 kb on - strand at 334.991 kb on - strand at 334.991 kb on - strand at 334.991 kb on - strand at 335.170 kb on + strand at 335.170 kb on + strand, within H281DRAFT_00310 at 335.170 kb on + strand, within H281DRAFT_00310 at 335.352 kb on + strand, within H281DRAFT_00310 at 335.352 kb on + strand, within H281DRAFT_00310 at 335.353 kb on - strand, within H281DRAFT_00310 at 335.612 kb on - strand, within H281DRAFT_00310 at 335.768 kb on - strand at 335.809 kb on + strand at 335.809 kb on + strand at 336.028 kb on + strand at 336.029 kb on - strand at 336.151 kb on + strand at 336.151 kb on + strand at 336.152 kb on - strand at 336.152 kb on - strand at 336.152 kb on - strand at 336.152 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 333,938 + +2.8 334,209 - H281DRAFT_00309 0.20 +0.1 334,238 + H281DRAFT_00309 0.23 -0.7 334,351 + H281DRAFT_00309 0.35 -0.4 334,352 - H281DRAFT_00309 0.35 -0.2 334,367 + H281DRAFT_00309 0.37 +0.2 334,367 + H281DRAFT_00309 0.37 -0.3 334,368 - H281DRAFT_00309 0.37 -1.5 334,569 + H281DRAFT_00309 0.58 -0.3 334,570 - H281DRAFT_00309 0.58 -0.5 334,710 - H281DRAFT_00309 0.72 -0.2 334,710 - H281DRAFT_00309 0.72 +0.4 334,710 - H281DRAFT_00309 0.72 +0.3 334,710 - H281DRAFT_00309 0.72 -0.7 334,990 + -0.0 334,990 + +1.9 334,991 - -0.7 334,991 - -0.0 334,991 - -0.1 334,991 - +2.4 334,991 - +0.6 335,170 + +1.0 335,170 + H281DRAFT_00310 0.28 -0.7 335,170 + H281DRAFT_00310 0.28 +0.8 335,352 + H281DRAFT_00310 0.49 -0.7 335,352 + H281DRAFT_00310 0.49 +0.3 335,353 - H281DRAFT_00310 0.49 -1.0 335,612 - H281DRAFT_00310 0.79 -1.9 335,768 - +0.1 335,809 + -0.3 335,809 + -0.0 336,028 + +1.1 336,029 - +0.4 336,151 + +0.3 336,151 + -0.2 336,152 - +0.0 336,152 - +0.7 336,152 - -0.2 336,152 - +1.1
Or see this region's nucleotide sequence