Experiment: Recovery broth with Rifampicin 3e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12920 and BBR_RS12925 are separated by 181 nucleotides BBR_RS12925 and BBR_RS12930 are separated by 273 nucleotides BBR_RS12930 and BBR_RS12935 are separated by 20 nucleotides
BBR_RS12920: BBR_RS12920 - glycine synthesis protein GlyXL <small>(from data)</small>, at 650,899 to 652,263
_RS12920
BBR_RS12925: BBR_RS12925 - hypothetical protein, at 652,445 to 652,648
_RS12925
BBR_RS12930: BBR_RS12930 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 652,922 to 653,584
_RS12930
BBR_RS12935: BBR_RS12935 - A/G-specific adenine glycosylase, at 653,605 to 654,567
_RS12935
Position (kb) 652
653
654 Strain fitness (log2 ratio) -2
-1
0
1
2 at 651.934 kb on - strand, within BBR_RS12920 at 651.949 kb on + strand, within BBR_RS12920 at 651.950 kb on + strand, within BBR_RS12920 at 651.950 kb on + strand, within BBR_RS12920 at 651.951 kb on + strand, within BBR_RS12920 at 651.958 kb on + strand, within BBR_RS12920 at 651.958 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.973 kb on + strand, within BBR_RS12920 at 651.980 kb on - strand, within BBR_RS12920 at 651.981 kb on - strand, within BBR_RS12920 at 651.981 kb on - strand, within BBR_RS12920 at 651.991 kb on - strand, within BBR_RS12920 at 651.998 kb on + strand, within BBR_RS12920 at 652.028 kb on + strand, within BBR_RS12920 at 652.033 kb on + strand, within BBR_RS12920 at 652.043 kb on + strand, within BBR_RS12920 at 652.069 kb on + strand, within BBR_RS12920 at 652.087 kb on - strand, within BBR_RS12920 at 652.089 kb on + strand, within BBR_RS12920 at 652.092 kb on + strand, within BBR_RS12920 at 652.094 kb on + strand, within BBR_RS12920 at 652.113 kb on - strand, within BBR_RS12920 at 652.130 kb on - strand at 652.156 kb on + strand at 652.156 kb on + strand at 652.199 kb on + strand at 652.201 kb on + strand at 652.201 kb on + strand at 652.262 kb on + strand at 652.273 kb on + strand at 652.375 kb on + strand at 652.493 kb on + strand, within BBR_RS12925 at 652.561 kb on - strand, within BBR_RS12925 at 652.638 kb on + strand at 652.658 kb on - strand at 652.743 kb on + strand at 652.743 kb on - strand at 652.743 kb on - strand at 652.744 kb on - strand at 652.749 kb on - strand at 652.830 kb on - strand at 652.845 kb on - strand at 652.849 kb on - strand at 652.849 kb on - strand at 652.913 kb on + strand at 652.949 kb on + strand at 652.953 kb on - strand at 652.957 kb on - strand at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 653.011 kb on + strand, within BBR_RS12930 at 653.013 kb on + strand, within BBR_RS12930 at 653.032 kb on + strand, within BBR_RS12930 at 653.068 kb on + strand, within BBR_RS12930 at 653.086 kb on + strand, within BBR_RS12930 at 653.096 kb on + strand, within BBR_RS12930 at 653.096 kb on + strand, within BBR_RS12930 at 653.135 kb on + strand, within BBR_RS12930 at 653.157 kb on - strand, within BBR_RS12930 at 653.187 kb on + strand, within BBR_RS12930 at 653.199 kb on - strand, within BBR_RS12930 at 653.204 kb on - strand, within BBR_RS12930 at 653.204 kb on - strand, within BBR_RS12930 at 653.223 kb on + strand, within BBR_RS12930 at 653.225 kb on - strand, within BBR_RS12930 at 653.235 kb on - strand, within BBR_RS12930 at 653.235 kb on - strand, within BBR_RS12930 at 653.242 kb on + strand, within BBR_RS12930 at 653.243 kb on + strand, within BBR_RS12930 at 653.251 kb on - strand, within BBR_RS12930 at 653.263 kb on + strand, within BBR_RS12930 at 653.271 kb on - strand, within BBR_RS12930 at 653.276 kb on - strand, within BBR_RS12930 at 653.278 kb on + strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.368 kb on + strand, within BBR_RS12930 at 653.370 kb on + strand, within BBR_RS12930 at 653.371 kb on - strand, within BBR_RS12930 at 653.378 kb on + strand, within BBR_RS12930 at 653.378 kb on - strand, within BBR_RS12930 at 653.389 kb on + strand, within BBR_RS12930 at 653.399 kb on - strand, within BBR_RS12930 at 653.421 kb on + strand, within BBR_RS12930 at 653.429 kb on + strand, within BBR_RS12930 at 653.451 kb on + strand, within BBR_RS12930 at 653.459 kb on - strand, within BBR_RS12930 at 653.483 kb on - strand, within BBR_RS12930 at 653.602 kb on - strand at 653.605 kb on + strand at 653.639 kb on + strand at 653.639 kb on + strand at 653.639 kb on - strand at 653.643 kb on + strand at 653.651 kb on - strand at 653.685 kb on + strand at 653.685 kb on + strand at 653.692 kb on - strand at 653.711 kb on + strand, within BBR_RS12935 at 653.711 kb on + strand, within BBR_RS12935 at 653.715 kb on + strand, within BBR_RS12935 at 653.736 kb on + strand, within BBR_RS12935 at 653.736 kb on + strand, within BBR_RS12935 at 653.780 kb on + strand, within BBR_RS12935 at 653.780 kb on + strand, within BBR_RS12935 at 653.787 kb on + strand, within BBR_RS12935 at 653.788 kb on - strand, within BBR_RS12935 at 653.788 kb on - strand, within BBR_RS12935 at 653.788 kb on - strand, within BBR_RS12935 at 653.797 kb on - strand, within BBR_RS12935 at 653.797 kb on - strand, within BBR_RS12935 at 653.809 kb on + strand, within BBR_RS12935 at 653.809 kb on + strand, within BBR_RS12935 at 653.809 kb on + strand, within BBR_RS12935 at 653.816 kb on - strand, within BBR_RS12935 at 653.816 kb on - strand, within BBR_RS12935 at 653.816 kb on - strand, within BBR_RS12935 at 653.818 kb on + strand, within BBR_RS12935 at 653.826 kb on - strand, within BBR_RS12935 at 653.826 kb on - strand, within BBR_RS12935 at 653.916 kb on + strand, within BBR_RS12935 at 653.916 kb on + strand, within BBR_RS12935 at 653.916 kb on + strand, within BBR_RS12935 at 653.930 kb on - strand, within BBR_RS12935 at 653.946 kb on + strand, within BBR_RS12935 at 653.959 kb on - strand, within BBR_RS12935 at 653.963 kb on + strand, within BBR_RS12935 at 653.966 kb on - strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.985 kb on - strand, within BBR_RS12935 at 653.985 kb on - strand, within BBR_RS12935 at 654.052 kb on - strand, within BBR_RS12935 at 654.052 kb on - strand, within BBR_RS12935 at 654.084 kb on - strand, within BBR_RS12935 at 654.110 kb on - strand, within BBR_RS12935 at 654.123 kb on + strand, within BBR_RS12935 at 654.124 kb on + strand, within BBR_RS12935 at 654.124 kb on - strand, within BBR_RS12935 at 654.147 kb on + strand, within BBR_RS12935 at 654.147 kb on + strand, within BBR_RS12935 at 654.161 kb on + strand, within BBR_RS12935 at 654.190 kb on + strand, within BBR_RS12935 at 654.195 kb on + strand, within BBR_RS12935 at 654.195 kb on - strand, within BBR_RS12935 at 654.226 kb on - strand, within BBR_RS12935 at 654.240 kb on - strand, within BBR_RS12935 at 654.245 kb on + strand, within BBR_RS12935 at 654.260 kb on - strand, within BBR_RS12935 at 654.260 kb on - strand, within BBR_RS12935 at 654.262 kb on + strand, within BBR_RS12935 at 654.270 kb on - strand, within BBR_RS12935 at 654.312 kb on + strand, within BBR_RS12935 at 654.332 kb on + strand, within BBR_RS12935 at 654.332 kb on + strand, within BBR_RS12935 at 654.369 kb on + strand, within BBR_RS12935 at 654.377 kb on + strand, within BBR_RS12935 at 654.382 kb on + strand, within BBR_RS12935 at 654.387 kb on + strand, within BBR_RS12935 at 654.417 kb on - strand, within BBR_RS12935 at 654.480 kb on - strand at 654.535 kb on - strand at 654.539 kb on + strand at 654.555 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Rifampicin 3e-04 mM remove 651,934 - BBR_RS12920 0.76 -0.8 651,949 + BBR_RS12920 0.77 -0.4 651,950 + BBR_RS12920 0.77 +0.3 651,950 + BBR_RS12920 0.77 +0.4 651,951 + BBR_RS12920 0.77 -0.6 651,958 + BBR_RS12920 0.78 -0.7 651,958 + BBR_RS12920 0.78 -0.0 651,966 + BBR_RS12920 0.78 +1.7 651,966 + BBR_RS12920 0.78 +0.2 651,966 + BBR_RS12920 0.78 -0.5 651,973 + BBR_RS12920 0.79 +0.5 651,980 - BBR_RS12920 0.79 -1.1 651,981 - BBR_RS12920 0.79 +1.9 651,981 - BBR_RS12920 0.79 -0.1 651,991 - BBR_RS12920 0.80 +0.4 651,998 + BBR_RS12920 0.81 +0.0 652,028 + BBR_RS12920 0.83 +0.8 652,033 + BBR_RS12920 0.83 +0.6 652,043 + BBR_RS12920 0.84 -0.5 652,069 + BBR_RS12920 0.86 +0.1 652,087 - BBR_RS12920 0.87 -0.3 652,089 + BBR_RS12920 0.87 +0.1 652,092 + BBR_RS12920 0.87 +1.1 652,094 + BBR_RS12920 0.88 +0.0 652,113 - BBR_RS12920 0.89 +0.2 652,130 - -0.3 652,156 + -0.0 652,156 + +0.6 652,199 + -0.1 652,201 + +0.0 652,201 + +0.5 652,262 + -0.3 652,273 + -0.7 652,375 + +0.4 652,493 + BBR_RS12925 0.24 +0.3 652,561 - BBR_RS12925 0.57 -0.3 652,638 + +1.5 652,658 - +0.3 652,743 + +1.5 652,743 - -0.2 652,743 - +0.1 652,744 - +1.2 652,749 - +0.1 652,830 - +0.0 652,845 - +0.2 652,849 - +1.5 652,849 - +1.0 652,913 + +1.1 652,949 + -0.7 652,953 - +1.0 652,957 - +0.3 652,990 + BBR_RS12930 0.10 +0.2 652,990 + BBR_RS12930 0.10 +0.6 652,990 + BBR_RS12930 0.10 +0.2 652,990 + BBR_RS12930 0.10 +1.6 652,990 + BBR_RS12930 0.10 +0.7 652,990 + BBR_RS12930 0.10 -0.8 652,990 + BBR_RS12930 0.10 +0.1 652,990 + BBR_RS12930 0.10 -0.1 652,998 - BBR_RS12930 0.11 +0.2 652,998 - BBR_RS12930 0.11 +0.7 652,998 - BBR_RS12930 0.11 -0.3 652,998 - BBR_RS12930 0.11 +0.6 652,998 - BBR_RS12930 0.11 +0.5 652,998 - BBR_RS12930 0.11 +1.4 652,998 - BBR_RS12930 0.11 +0.7 652,998 - BBR_RS12930 0.11 +0.3 652,998 - BBR_RS12930 0.11 +1.1 652,998 - BBR_RS12930 0.11 -0.0 652,998 - BBR_RS12930 0.11 -0.7 652,998 - BBR_RS12930 0.11 +0.7 652,998 - BBR_RS12930 0.11 +0.5 653,011 + BBR_RS12930 0.13 -0.2 653,013 + BBR_RS12930 0.14 +0.3 653,032 + BBR_RS12930 0.17 +0.9 653,068 + BBR_RS12930 0.22 +0.5 653,086 + BBR_RS12930 0.25 -0.0 653,096 + BBR_RS12930 0.26 +0.7 653,096 + BBR_RS12930 0.26 +0.3 653,135 + BBR_RS12930 0.32 +0.7 653,157 - BBR_RS12930 0.35 +0.6 653,187 + BBR_RS12930 0.40 -0.3 653,199 - BBR_RS12930 0.42 +0.2 653,204 - BBR_RS12930 0.43 +1.3 653,204 - BBR_RS12930 0.43 +1.6 653,223 + BBR_RS12930 0.45 +1.4 653,225 - BBR_RS12930 0.46 -1.1 653,235 - BBR_RS12930 0.47 +0.1 653,235 - BBR_RS12930 0.47 +0.6 653,242 + BBR_RS12930 0.48 +0.7 653,243 + BBR_RS12930 0.48 +0.3 653,251 - BBR_RS12930 0.50 -0.1 653,263 + BBR_RS12930 0.51 +2.3 653,271 - BBR_RS12930 0.53 +0.3 653,276 - BBR_RS12930 0.53 -0.6 653,278 + BBR_RS12930 0.54 +0.1 653,286 - BBR_RS12930 0.55 -1.4 653,286 - BBR_RS12930 0.55 +0.1 653,286 - BBR_RS12930 0.55 +0.3 653,286 - BBR_RS12930 0.55 -0.1 653,286 - BBR_RS12930 0.55 +0.2 653,286 - BBR_RS12930 0.55 +0.4 653,368 + BBR_RS12930 0.67 +0.7 653,370 + BBR_RS12930 0.68 -0.1 653,371 - BBR_RS12930 0.68 +1.4 653,378 + BBR_RS12930 0.69 -2.7 653,378 - BBR_RS12930 0.69 +1.0 653,389 + BBR_RS12930 0.70 -0.1 653,399 - BBR_RS12930 0.72 +0.6 653,421 + BBR_RS12930 0.75 +0.2 653,429 + BBR_RS12930 0.76 -0.7 653,451 + BBR_RS12930 0.80 -1.1 653,459 - BBR_RS12930 0.81 +2.1 653,483 - BBR_RS12930 0.85 +0.0 653,602 - +0.0 653,605 + +1.2 653,639 + +0.0 653,639 + +0.1 653,639 - -0.4 653,643 + -0.6 653,651 - +0.5 653,685 + -0.6 653,685 + -0.9 653,692 - -0.2 653,711 + BBR_RS12935 0.11 +0.0 653,711 + BBR_RS12935 0.11 -1.3 653,715 + BBR_RS12935 0.11 -0.1 653,736 + BBR_RS12935 0.14 +0.1 653,736 + BBR_RS12935 0.14 +0.8 653,780 + BBR_RS12935 0.18 +0.2 653,780 + BBR_RS12935 0.18 +0.4 653,787 + BBR_RS12935 0.19 -0.9 653,788 - BBR_RS12935 0.19 +0.7 653,788 - BBR_RS12935 0.19 +0.4 653,788 - BBR_RS12935 0.19 -0.5 653,797 - BBR_RS12935 0.20 +0.5 653,797 - BBR_RS12935 0.20 +1.5 653,809 + BBR_RS12935 0.21 +1.9 653,809 + BBR_RS12935 0.21 -0.3 653,809 + BBR_RS12935 0.21 +0.3 653,816 - BBR_RS12935 0.22 +0.7 653,816 - BBR_RS12935 0.22 -0.5 653,816 - BBR_RS12935 0.22 -0.3 653,818 + BBR_RS12935 0.22 -0.3 653,826 - BBR_RS12935 0.23 +0.2 653,826 - BBR_RS12935 0.23 -0.7 653,916 + BBR_RS12935 0.32 +0.2 653,916 + BBR_RS12935 0.32 +0.9 653,916 + BBR_RS12935 0.32 -0.3 653,930 - BBR_RS12935 0.34 +0.7 653,946 + BBR_RS12935 0.35 +0.1 653,959 - BBR_RS12935 0.37 -0.2 653,963 + BBR_RS12935 0.37 -0.2 653,966 - BBR_RS12935 0.37 +1.1 653,977 + BBR_RS12935 0.39 +1.0 653,977 + BBR_RS12935 0.39 -0.2 653,977 + BBR_RS12935 0.39 -1.6 653,977 + BBR_RS12935 0.39 +0.1 653,977 + BBR_RS12935 0.39 +0.8 653,977 + BBR_RS12935 0.39 +1.0 653,985 - BBR_RS12935 0.39 +0.8 653,985 - BBR_RS12935 0.39 -0.6 654,052 - BBR_RS12935 0.46 -0.0 654,052 - BBR_RS12935 0.46 -0.0 654,084 - BBR_RS12935 0.50 +0.6 654,110 - BBR_RS12935 0.52 +0.2 654,123 + BBR_RS12935 0.54 -0.1 654,124 + BBR_RS12935 0.54 -0.7 654,124 - BBR_RS12935 0.54 -0.8 654,147 + BBR_RS12935 0.56 -0.5 654,147 + BBR_RS12935 0.56 +0.0 654,161 + BBR_RS12935 0.58 +1.0 654,190 + BBR_RS12935 0.61 +0.5 654,195 + BBR_RS12935 0.61 +0.5 654,195 - BBR_RS12935 0.61 -0.0 654,226 - BBR_RS12935 0.64 +0.2 654,240 - BBR_RS12935 0.66 +1.2 654,245 + BBR_RS12935 0.66 +0.4 654,260 - BBR_RS12935 0.68 +0.7 654,260 - BBR_RS12935 0.68 -0.3 654,262 + BBR_RS12935 0.68 +0.5 654,270 - BBR_RS12935 0.69 +0.9 654,312 + BBR_RS12935 0.73 -0.5 654,332 + BBR_RS12935 0.75 -0.3 654,332 + BBR_RS12935 0.75 +0.8 654,369 + BBR_RS12935 0.79 +0.6 654,377 + BBR_RS12935 0.80 +0.4 654,382 + BBR_RS12935 0.81 +0.6 654,387 + BBR_RS12935 0.81 -0.8 654,417 - BBR_RS12935 0.84 +0.9 654,480 - +0.3 654,535 - +0.1 654,539 + +0.5 654,555 + +0.3 654,577 + -0.0 654,577 + +0.0 654,577 + -0.7 654,577 + +0.5 654,577 + +0.3 654,577 + -1.0
Or see this region's nucleotide sequence