Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS13525 and ABZR86_RS13530 are separated by 78 nucleotides ABZR86_RS13530 and ABZR86_RS13535 are separated by 144 nucleotides
ABZR86_RS13525: ABZR86_RS13525 - oxygen-dependent coproporphyrinogen oxidase, at 3,029,524 to 3,030,426
_RS13525
ABZR86_RS13530: ABZR86_RS13530 - YdcF family protein, at 3,030,505 to 3,031,215
_RS13530
ABZR86_RS13535: ABZR86_RS13535 - beta-ketoacyl-[acyl-carrier-protein] synthase family protein, at 3,031,360 to 3,032,544
_RS13535
Position (kb)
3030
3031
3032 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.462 kb on + strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.463 kb on - strand at 3030.506 kb on + strand at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.581 kb on + strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.582 kb on - strand, within ABZR86_RS13530 at 3030.707 kb on + strand, within ABZR86_RS13530 at 3030.707 kb on + strand, within ABZR86_RS13530 at 3030.977 kb on + strand, within ABZR86_RS13530 at 3030.978 kb on - strand, within ABZR86_RS13530 at 3030.978 kb on - strand, within ABZR86_RS13530 at 3030.978 kb on - strand, within ABZR86_RS13530 at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.255 kb on + strand at 3031.256 kb on - strand at 3031.256 kb on - strand at 3031.256 kb on - strand at 3031.256 kb on - strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.275 kb on + strand at 3031.276 kb on - strand at 3031.276 kb on - strand at 3031.288 kb on + strand at 3031.288 kb on + strand at 3031.288 kb on + strand at 3031.288 kb on + strand at 3031.289 kb on - strand at 3031.289 kb on - strand at 3031.289 kb on - strand at 3031.296 kb on + strand at 3031.296 kb on + strand at 3031.296 kb on + strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.297 kb on - strand at 3031.336 kb on + strand at 3031.336 kb on + strand at 3031.384 kb on + strand at 3031.384 kb on + strand at 3031.384 kb on + strand at 3031.385 kb on - strand at 3031.385 kb on - strand at 3031.386 kb on + strand at 3031.386 kb on + strand at 3031.386 kb on + strand at 3031.387 kb on - strand at 3031.387 kb on - strand at 3031.387 kb on - strand at 3032.092 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.209 kb on + strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535 at 3032.210 kb on - strand, within ABZR86_RS13535
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 3,030,462 + +1.3 3,030,462 + -0.3 3,030,462 + +0.5 3,030,462 + -0.9 3,030,462 + +0.1 3,030,462 + -0.1 3,030,462 + +0.6 3,030,463 - +0.1 3,030,463 - +0.1 3,030,463 - +0.4 3,030,463 - +0.0 3,030,463 - +0.3 3,030,463 - -0.2 3,030,463 - +0.2 3,030,463 - +0.7 3,030,463 - +0.1 3,030,506 + -0.1 3,030,581 + ABZR86_RS13530 0.11 -0.4 3,030,581 + ABZR86_RS13530 0.11 -0.2 3,030,581 + ABZR86_RS13530 0.11 +0.6 3,030,581 + ABZR86_RS13530 0.11 -0.6 3,030,581 + ABZR86_RS13530 0.11 +0.4 3,030,581 + ABZR86_RS13530 0.11 -0.9 3,030,581 + ABZR86_RS13530 0.11 +0.6 3,030,581 + ABZR86_RS13530 0.11 +0.1 3,030,581 + ABZR86_RS13530 0.11 -0.1 3,030,581 + ABZR86_RS13530 0.11 -0.1 3,030,581 + ABZR86_RS13530 0.11 +0.2 3,030,582 - ABZR86_RS13530 0.11 -0.7 3,030,582 - ABZR86_RS13530 0.11 -0.3 3,030,582 - ABZR86_RS13530 0.11 -0.1 3,030,582 - ABZR86_RS13530 0.11 +1.4 3,030,582 - ABZR86_RS13530 0.11 +0.4 3,030,582 - ABZR86_RS13530 0.11 +0.5 3,030,582 - ABZR86_RS13530 0.11 +0.3 3,030,582 - ABZR86_RS13530 0.11 +0.0 3,030,582 - ABZR86_RS13530 0.11 +0.1 3,030,582 - ABZR86_RS13530 0.11 +0.5 3,030,582 - ABZR86_RS13530 0.11 +0.3 3,030,582 - ABZR86_RS13530 0.11 -0.0 3,030,707 + ABZR86_RS13530 0.28 +0.0 3,030,707 + ABZR86_RS13530 0.28 -0.0 3,030,977 + ABZR86_RS13530 0.66 +0.2 3,030,978 - ABZR86_RS13530 0.67 -0.3 3,030,978 - ABZR86_RS13530 0.67 +0.2 3,030,978 - ABZR86_RS13530 0.67 -0.1 3,031,255 + -0.3 3,031,255 + -1.2 3,031,255 + -0.8 3,031,255 + +0.2 3,031,255 + +0.6 3,031,255 + +0.7 3,031,255 + +1.9 3,031,255 + -0.6 3,031,256 - -0.3 3,031,256 - -0.0 3,031,256 - -0.2 3,031,256 - +0.1 3,031,275 + +0.4 3,031,275 + -0.7 3,031,275 + -0.4 3,031,275 + +0.6 3,031,275 + +0.5 3,031,275 + -0.4 3,031,275 + -0.4 3,031,275 + +0.3 3,031,275 + +0.5 3,031,276 - -0.4 3,031,276 - +0.4 3,031,288 + -0.1 3,031,288 + -0.3 3,031,288 + +0.2 3,031,288 + +0.1 3,031,289 - -0.1 3,031,289 - -0.3 3,031,289 - +0.0 3,031,296 + -0.1 3,031,296 + -0.2 3,031,296 + +0.6 3,031,297 - -0.2 3,031,297 - +0.2 3,031,297 - +1.9 3,031,297 - +0.0 3,031,297 - +0.2 3,031,297 - +0.1 3,031,297 - +0.2 3,031,297 - -0.5 3,031,297 - -0.0 3,031,297 - -0.2 3,031,297 - +0.5 3,031,297 - -0.0 3,031,297 - +0.4 3,031,336 + +0.4 3,031,336 + +0.3 3,031,384 + -3.9 3,031,384 + -1.7 3,031,384 + -2.7 3,031,385 - -0.5 3,031,385 - -0.6 3,031,386 + -1.5 3,031,386 + -1.0 3,031,386 + -2.0 3,031,387 - -0.9 3,031,387 - -1.4 3,031,387 - -1.6 3,032,092 + ABZR86_RS13535 0.62 -0.2 3,032,209 + ABZR86_RS13535 0.72 -2.3 3,032,209 + ABZR86_RS13535 0.72 -0.6 3,032,209 + ABZR86_RS13535 0.72 -0.6 3,032,209 + ABZR86_RS13535 0.72 -1.5 3,032,209 + ABZR86_RS13535 0.72 -1.2 3,032,209 + ABZR86_RS13535 0.72 -1.1 3,032,209 + ABZR86_RS13535 0.72 -1.4 3,032,209 + ABZR86_RS13535 0.72 +0.1 3,032,210 - ABZR86_RS13535 0.72 -1.2 3,032,210 - ABZR86_RS13535 0.72 -1.0 3,032,210 - ABZR86_RS13535 0.72 -0.9 3,032,210 - ABZR86_RS13535 0.72 -1.8 3,032,210 - ABZR86_RS13535 0.72 -0.5 3,032,210 - ABZR86_RS13535 0.72 -2.2
Or see this region's nucleotide sequence