Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2439 and Shew_2440 are separated by 30 nucleotides Shew_2440 and Shew_2441 are separated by 11 nucleotides Shew_2441 and Shew_2442 are separated by 23 nucleotides
Shew_2439: Shew_2439 - RND family efflux transporter MFP subunit (RefSeq), at 2,866,545 to 2,867,561
_2439
Shew_2440: Shew_2440 - acriflavin resistance protein (RefSeq), at 2,867,592 to 2,870,846
_2440
Shew_2441: Shew_2441 - hypothetical protein (RefSeq), at 2,870,858 to 2,871,070
_2441
Shew_2442: Shew_2442 - rhodanese domain-containing protein (RefSeq), at 2,871,094 to 2,871,477
_2442
Position (kb)
2867
2868
2869
2870
2871 Strain fitness (log2 ratio)
-1
0
1 at 2866.651 kb on - strand, within Shew_2439 at 2866.874 kb on + strand, within Shew_2439 at 2867.867 kb on + strand at 2868.018 kb on + strand, within Shew_2440 at 2868.486 kb on + strand, within Shew_2440 at 2869.151 kb on + strand, within Shew_2440 at 2869.151 kb on + strand, within Shew_2440 at 2869.487 kb on + strand, within Shew_2440 at 2869.687 kb on - strand, within Shew_2440 at 2870.478 kb on + strand, within Shew_2440 at 2870.536 kb on + strand at 2870.848 kb on - strand at 2871.058 kb on - strand at 2871.280 kb on + strand, within Shew_2442 at 2871.280 kb on + strand, within Shew_2442 at 2871.400 kb on + strand, within Shew_2442 at 2871.400 kb on + strand, within Shew_2442 at 2871.400 kb on + strand, within Shew_2442 at 2871.400 kb on + strand, within Shew_2442 at 2871.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,866,651 - Shew_2439 0.10 +0.8 2,866,874 + Shew_2439 0.32 +0.5 2,867,867 + -0.2 2,868,018 + Shew_2440 0.13 -0.1 2,868,486 + Shew_2440 0.27 -1.1 2,869,151 + Shew_2440 0.48 -0.4 2,869,151 + Shew_2440 0.48 -0.9 2,869,487 + Shew_2440 0.58 -0.2 2,869,687 - Shew_2440 0.64 -0.0 2,870,478 + Shew_2440 0.89 -0.2 2,870,536 + +0.9 2,870,848 - -1.1 2,871,058 - +0.0 2,871,280 + Shew_2442 0.48 -0.2 2,871,280 + Shew_2442 0.48 +0.2 2,871,400 + Shew_2442 0.80 +0.1 2,871,400 + Shew_2442 0.80 +0.2 2,871,400 + Shew_2442 0.80 +0.7 2,871,400 + Shew_2442 0.80 +0.2 2,871,697 - +0.1
Or see this region's nucleotide sequence