Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1999 and Shew_R0065 are separated by 395 nucleotides Shew_R0065 and Shew_R0066 are separated by 38 nucleotides Shew_R0066 and Shew_R0067 are separated by 35 nucleotides Shew_R0067 and Shew_2000 are separated by 233 nucleotides Shew_2000 and Shew_2001 are separated by 236 nucleotides
Shew_1999: Shew_1999 - aromatic amino acid aminotransferase (RefSeq), at 2,310,338 to 2,311,531
_1999
Shew_R0065: Shew_R0065 - tRNA-Leu (RefSeq), at 2,311,927 to 2,312,013
_R0065
Shew_R0066: Shew_R0066 - tRNA-Cys (RefSeq), at 2,312,052 to 2,312,125
_R0066
Shew_R0067: Shew_R0067 - tRNA-Gly (RefSeq), at 2,312,161 to 2,312,236
_R0067
Shew_2000: Shew_2000 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,312,470 to 2,313,906
_2000
Shew_2001: Shew_2001 - hypothetical protein (RefSeq), at 2,314,143 to 2,314,949
_2001
Position (kb)
2312
2313
2314 Strain fitness (log2 ratio)
-2
-1
0
1 at 2311.513 kb on - strand at 2311.574 kb on + strand at 2311.574 kb on + strand at 2312.691 kb on + strand, within Shew_2000 at 2313.180 kb on - strand, within Shew_2000 at 2313.922 kb on - strand at 2313.972 kb on + strand at 2313.972 kb on + strand at 2313.980 kb on - strand at 2314.130 kb on + strand at 2314.466 kb on + strand, within Shew_2001 at 2314.479 kb on + strand, within Shew_2001 at 2314.650 kb on - strand, within Shew_2001
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,311,513 - -0.9 2,311,574 + -2.5 2,311,574 + -1.1 2,312,691 + Shew_2000 0.15 -1.5 2,313,180 - Shew_2000 0.49 -0.6 2,313,922 - +1.5 2,313,972 + -0.9 2,313,972 + +0.1 2,313,980 - +0.7 2,314,130 + +0.5 2,314,466 + Shew_2001 0.40 +1.2 2,314,479 + Shew_2001 0.42 +0.3 2,314,650 - Shew_2001 0.63 +0.3
Or see this region's nucleotide sequence