Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1929 and Shew_R0064 are separated by 135 nucleotides Shew_R0064 and Shew_1930 are separated by 329 nucleotides Shew_1930 and Shew_1931 are separated by 200 nucleotides Shew_1931 and cls are separated by 166 nucleotides
Shew_1929: Shew_1929 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,227,766 to 2,229,754
_1929
Shew_R0064: Shew_R0064 - tRNA-Ser (RefSeq), at 2,229,890 to 2,229,977
_R0064
Shew_1930: Shew_1930 - CopG/DNA-binding domain-containing protein (RefSeq), at 2,230,307 to 2,230,504
_1930
Shew_1931: Shew_1931 - alkylphosphonate utilization operon protein PhnA (RefSeq), at 2,230,705 to 2,231,049
_1931
Shew_1932: cls - cardiolipin synthetase (RefSeq), at 2,231,216 to 2,232,814
cls
Position (kb)
2230
2231
2232 Strain fitness (log2 ratio)
-1
0
1
2 at 2230.139 kb on - strand at 2230.744 kb on + strand, within Shew_1931 at 2230.888 kb on - strand, within Shew_1931 at 2231.505 kb on + strand, within cls at 2231.505 kb on + strand, within cls at 2231.513 kb on - strand, within cls at 2231.513 kb on - strand, within cls at 2231.641 kb on + strand, within cls
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,230,139 - +0.2 2,230,744 + Shew_1931 0.11 -1.0 2,230,888 - Shew_1931 0.53 +0.8 2,231,505 + cls Shew_1932 0.18 +1.6 2,231,505 + cls Shew_1932 0.18 +1.0 2,231,513 - cls Shew_1932 0.19 +0.4 2,231,513 - cls Shew_1932 0.19 +0.0 2,231,641 + cls Shew_1932 0.27 +2.0
Or see this region's nucleotide sequence