Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1819 and prpB are separated by 12 nucleotides prpB and Shew_1821 are separated by 101 nucleotides Shew_1821 and Shew_1822 are separated by 86 nucleotides
Shew_1819: Shew_1819 - GntR family transcriptional regulator (RefSeq), at 2,092,309 to 2,092,977
_1819
Shew_1820: prpB - 2-methylisocitrate lyase (RefSeq), at 2,092,990 to 2,093,868
prpB
Shew_1821: Shew_1821 - 2-methylcitrate synthase/citrate synthase II (RefSeq), at 2,093,970 to 2,095,094
_1821
Shew_1822: Shew_1822 - aconitate hydratase (RefSeq), at 2,095,181 to 2,097,769
_1822
Position (kb)
2093
2094
2095
2096 Strain fitness (log2 ratio)
-1
0
1 at 2093.552 kb on - strand, within prpB at 2093.582 kb on + strand, within prpB at 2093.677 kb on - strand, within prpB at 2093.681 kb on + strand, within prpB at 2093.702 kb on - strand, within prpB at 2093.933 kb on + strand at 2093.933 kb on + strand at 2093.941 kb on - strand at 2094.037 kb on - strand at 2094.445 kb on + strand, within Shew_1821 at 2094.804 kb on - strand, within Shew_1821 at 2095.654 kb on - strand, within Shew_1822
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,093,552 - prpB Shew_1820 0.64 +1.1 2,093,582 + prpB Shew_1820 0.67 +0.6 2,093,677 - prpB Shew_1820 0.78 +1.0 2,093,681 + prpB Shew_1820 0.79 -0.1 2,093,702 - prpB Shew_1820 0.81 +0.8 2,093,933 + -0.4 2,093,933 + -0.4 2,093,941 - -0.4 2,094,037 - -0.5 2,094,445 + Shew_1821 0.42 -0.3 2,094,804 - Shew_1821 0.74 -1.7 2,095,654 - Shew_1822 0.18 -0.5
Or see this region's nucleotide sequence