Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_925 and Pf6N2E2_926 are separated by 779 nucleotides Pf6N2E2_926 and Pf6N2E2_927 are separated by 722 nucleotides
Pf6N2E2_925 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 1,088,837 to 1,089,595
_925
Pf6N2E2_926 - NADH dehydrogenase (EC 1.6.99.3), at 1,090,375 to 1,091,172
_926
Pf6N2E2_927 - (AJ250023) putative polyketide synthase, at 1,091,895 to 1,092,041
_927
Position (kb)
1090
1091
1092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1089.387 kb on + strand, within Pf6N2E2_925 at 1089.388 kb on - strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.391 kb on + strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.392 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.395 kb on - strand, within Pf6N2E2_925 at 1089.447 kb on + strand, within Pf6N2E2_925 at 1089.575 kb on + strand at 1089.601 kb on - strand at 1089.607 kb on + strand at 1089.683 kb on + strand at 1089.787 kb on + strand at 1089.871 kb on - strand at 1089.879 kb on + strand at 1089.879 kb on + strand at 1089.880 kb on - strand at 1089.886 kb on + strand at 1089.886 kb on + strand at 1089.887 kb on - strand at 1089.891 kb on + strand at 1089.891 kb on + strand at 1089.891 kb on + strand at 1089.892 kb on - strand at 1089.892 kb on - strand at 1089.892 kb on - strand at 1089.892 kb on - strand at 1089.892 kb on - strand at 1089.892 kb on - strand at 1089.896 kb on - strand at 1090.151 kb on + strand at 1090.151 kb on + strand at 1090.489 kb on - strand, within Pf6N2E2_926 at 1090.509 kb on - strand, within Pf6N2E2_926 at 1090.530 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.646 kb on + strand, within Pf6N2E2_926 at 1090.647 kb on - strand, within Pf6N2E2_926 at 1090.647 kb on - strand, within Pf6N2E2_926 at 1090.647 kb on - strand, within Pf6N2E2_926 at 1090.647 kb on - strand, within Pf6N2E2_926 at 1090.762 kb on + strand, within Pf6N2E2_926 at 1090.763 kb on - strand, within Pf6N2E2_926 at 1090.773 kb on + strand, within Pf6N2E2_926 at 1090.774 kb on - strand, within Pf6N2E2_926 at 1090.798 kb on + strand, within Pf6N2E2_926 at 1090.798 kb on + strand, within Pf6N2E2_926 at 1090.799 kb on - strand, within Pf6N2E2_926 at 1090.807 kb on - strand, within Pf6N2E2_926 at 1090.815 kb on + strand, within Pf6N2E2_926 at 1090.815 kb on + strand, within Pf6N2E2_926 at 1090.895 kb on + strand, within Pf6N2E2_926 at 1090.948 kb on + strand, within Pf6N2E2_926 at 1090.949 kb on - strand, within Pf6N2E2_926 at 1090.949 kb on - strand, within Pf6N2E2_926 at 1090.964 kb on + strand, within Pf6N2E2_926 at 1090.998 kb on - strand, within Pf6N2E2_926 at 1091.020 kb on + strand, within Pf6N2E2_926 at 1091.028 kb on + strand, within Pf6N2E2_926 at 1091.028 kb on + strand, within Pf6N2E2_926 at 1091.045 kb on + strand, within Pf6N2E2_926 at 1091.046 kb on - strand, within Pf6N2E2_926 at 1091.046 kb on - strand, within Pf6N2E2_926 at 1091.046 kb on - strand, within Pf6N2E2_926 at 1091.127 kb on + strand at 1091.180 kb on + strand at 1091.181 kb on - strand at 1091.184 kb on + strand at 1091.241 kb on + strand at 1091.241 kb on + strand at 1091.242 kb on - strand at 1091.242 kb on - strand at 1091.274 kb on + strand at 1091.274 kb on + strand at 1091.274 kb on + strand at 1091.275 kb on - strand at 1091.275 kb on - strand at 1091.275 kb on - strand at 1091.279 kb on - strand at 1091.287 kb on + strand at 1091.287 kb on + strand at 1091.288 kb on - strand at 1091.291 kb on + strand at 1091.292 kb on - strand at 1091.336 kb on + strand at 1091.580 kb on + strand at 1091.607 kb on - strand at 1091.699 kb on + strand at 1091.699 kb on + strand at 1091.732 kb on + strand at 1091.732 kb on + strand at 1091.743 kb on + strand at 1091.743 kb on + strand at 1091.743 kb on + strand at 1091.743 kb on + strand at 1091.743 kb on + strand at 1091.749 kb on + strand at 1091.750 kb on - strand at 1091.750 kb on - strand at 1091.750 kb on - strand at 1091.756 kb on - strand at 1091.768 kb on + strand at 1091.769 kb on - strand at 1091.797 kb on + strand at 1091.797 kb on + strand at 1091.797 kb on + strand at 1091.797 kb on + strand at 1091.797 kb on + strand at 1091.798 kb on - strand at 1091.816 kb on + strand at 1091.868 kb on - strand at 1091.877 kb on + strand at 1091.877 kb on + strand at 1091.878 kb on - strand at 1091.959 kb on + strand, within Pf6N2E2_927 at 1091.959 kb on + strand, within Pf6N2E2_927 at 1091.996 kb on + strand, within Pf6N2E2_927 at 1091.997 kb on - strand, within Pf6N2E2_927 at 1092.064 kb on + strand at 1092.064 kb on + strand at 1092.064 kb on + strand at 1092.065 kb on - strand at 1092.065 kb on - strand at 1092.068 kb on + strand at 1092.068 kb on + strand at 1092.069 kb on - strand at 1092.069 kb on - strand at 1092.069 kb on - strand at 1092.080 kb on + strand at 1092.080 kb on + strand at 1092.080 kb on + strand at 1092.080 kb on + strand at 1092.080 kb on + strand at 1092.081 kb on - strand at 1092.110 kb on + strand at 1092.110 kb on + strand at 1092.111 kb on - strand at 1092.111 kb on - strand at 1092.111 kb on - strand at 1092.111 kb on - strand at 1092.114 kb on + strand at 1092.114 kb on + strand at 1092.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,089,387 + Pf6N2E2_925 0.72 +1.6 1,089,388 - Pf6N2E2_925 0.73 -0.3 1,089,391 + Pf6N2E2_925 0.73 +1.7 1,089,391 + Pf6N2E2_925 0.73 +0.5 1,089,391 + Pf6N2E2_925 0.73 +1.3 1,089,392 - Pf6N2E2_925 0.73 -0.3 1,089,392 - Pf6N2E2_925 0.73 -2.3 1,089,392 - Pf6N2E2_925 0.73 -0.7 1,089,392 - Pf6N2E2_925 0.73 -0.6 1,089,392 - Pf6N2E2_925 0.73 +0.0 1,089,395 - Pf6N2E2_925 0.74 +0.3 1,089,395 - Pf6N2E2_925 0.74 -0.3 1,089,395 - Pf6N2E2_925 0.74 -2.6 1,089,395 - Pf6N2E2_925 0.74 -0.6 1,089,395 - Pf6N2E2_925 0.74 +1.2 1,089,447 + Pf6N2E2_925 0.80 +1.4 1,089,575 + +0.2 1,089,601 - +0.0 1,089,607 + +1.0 1,089,683 + +1.9 1,089,787 + +0.9 1,089,871 - +0.4 1,089,879 + +3.1 1,089,879 + +2.4 1,089,880 - +1.3 1,089,886 + +1.3 1,089,886 + +1.1 1,089,887 - -0.2 1,089,891 + +2.3 1,089,891 + +4.6 1,089,891 + +2.6 1,089,892 - -0.5 1,089,892 - -2.4 1,089,892 - -0.3 1,089,892 - -0.1 1,089,892 - -1.4 1,089,892 - -0.5 1,089,896 - -1.2 1,090,151 + +4.8 1,090,151 + +4.3 1,090,489 - Pf6N2E2_926 0.14 -0.8 1,090,509 - Pf6N2E2_926 0.17 +0.6 1,090,530 + Pf6N2E2_926 0.19 +2.3 1,090,646 + Pf6N2E2_926 0.34 +1.4 1,090,646 + Pf6N2E2_926 0.34 +3.4 1,090,646 + Pf6N2E2_926 0.34 +2.6 1,090,646 + Pf6N2E2_926 0.34 +2.6 1,090,646 + Pf6N2E2_926 0.34 +4.0 1,090,646 + Pf6N2E2_926 0.34 +2.3 1,090,646 + Pf6N2E2_926 0.34 +1.2 1,090,647 - Pf6N2E2_926 0.34 -4.1 1,090,647 - Pf6N2E2_926 0.34 -1.6 1,090,647 - Pf6N2E2_926 0.34 -0.9 1,090,647 - Pf6N2E2_926 0.34 -0.3 1,090,762 + Pf6N2E2_926 0.48 +3.0 1,090,763 - Pf6N2E2_926 0.49 -2.3 1,090,773 + Pf6N2E2_926 0.50 +3.0 1,090,774 - Pf6N2E2_926 0.50 -1.3 1,090,798 + Pf6N2E2_926 0.53 +2.6 1,090,798 + Pf6N2E2_926 0.53 +2.7 1,090,799 - Pf6N2E2_926 0.53 -2.2 1,090,807 - Pf6N2E2_926 0.54 -0.1 1,090,815 + Pf6N2E2_926 0.55 +1.9 1,090,815 + Pf6N2E2_926 0.55 +2.5 1,090,895 + Pf6N2E2_926 0.65 +3.1 1,090,948 + Pf6N2E2_926 0.72 +3.4 1,090,949 - Pf6N2E2_926 0.72 +0.2 1,090,949 - Pf6N2E2_926 0.72 -1.7 1,090,964 + Pf6N2E2_926 0.74 +3.5 1,090,998 - Pf6N2E2_926 0.78 -0.1 1,091,020 + Pf6N2E2_926 0.81 +5.1 1,091,028 + Pf6N2E2_926 0.82 +5.1 1,091,028 + Pf6N2E2_926 0.82 +4.8 1,091,045 + Pf6N2E2_926 0.84 +4.2 1,091,046 - Pf6N2E2_926 0.84 -2.1 1,091,046 - Pf6N2E2_926 0.84 -0.9 1,091,046 - Pf6N2E2_926 0.84 -2.4 1,091,127 + +7.0 1,091,180 + +4.4 1,091,181 - -2.3 1,091,184 + +4.9 1,091,241 + +3.3 1,091,241 + +3.9 1,091,242 - +0.3 1,091,242 - -1.5 1,091,274 + +0.9 1,091,274 + +2.3 1,091,274 + +2.7 1,091,275 - -1.3 1,091,275 - +0.8 1,091,275 - -1.3 1,091,279 - -0.3 1,091,287 + +2.1 1,091,287 + +1.3 1,091,288 - -2.0 1,091,291 + +3.0 1,091,292 - -0.5 1,091,336 + -0.3 1,091,580 + +0.2 1,091,607 - -2.6 1,091,699 + +1.3 1,091,699 + +0.2 1,091,732 + -1.3 1,091,732 + +0.2 1,091,743 + +0.5 1,091,743 + -2.1 1,091,743 + -0.3 1,091,743 + -1.7 1,091,743 + +0.5 1,091,749 + -2.6 1,091,750 - -1.9 1,091,750 - +0.3 1,091,750 - -0.9 1,091,756 - -1.1 1,091,768 + -1.7 1,091,769 - -0.6 1,091,797 + -0.4 1,091,797 + -0.3 1,091,797 + -0.3 1,091,797 + -1.0 1,091,797 + -0.7 1,091,798 - -2.3 1,091,816 + -1.2 1,091,868 - -0.7 1,091,877 + +0.2 1,091,877 + -0.3 1,091,878 - -0.6 1,091,959 + Pf6N2E2_927 0.44 +1.0 1,091,959 + Pf6N2E2_927 0.44 -0.4 1,091,996 + Pf6N2E2_927 0.69 -1.6 1,091,997 - Pf6N2E2_927 0.69 -0.3 1,092,064 + -0.7 1,092,064 + -0.3 1,092,064 + -1.3 1,092,065 - +0.6 1,092,065 - +1.6 1,092,068 + -2.3 1,092,068 + -0.5 1,092,069 - -0.6 1,092,069 - +0.7 1,092,069 - +0.1 1,092,080 + -0.3 1,092,080 + +1.4 1,092,080 + -0.1 1,092,080 + -1.9 1,092,080 + +0.3 1,092,081 - -0.3 1,092,110 + +0.7 1,092,110 + -0.2 1,092,111 - -0.1 1,092,111 - -1.4 1,092,111 - -1.1 1,092,111 - -0.8 1,092,114 + +0.1 1,092,114 + -0.3 1,092,114 + -1.3
Or see this region's nucleotide sequence