Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_915 and Pf6N2E2_916 are separated by 854 nucleotides Pf6N2E2_916 and Pf6N2E2_917 are separated by 323 nucleotides
Pf6N2E2_915 - FIG00962566: hypothetical protein, at 1,075,284 to 1,075,613
_915
Pf6N2E2_916 - putative membrane protein, at 1,076,468 to 1,078,006
_916
Pf6N2E2_917 - FIG00959181: hypothetical protein, at 1,078,330 to 1,079,646
_917
Position (kb)
1076
1077
1078
1079 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1075.474 kb on - strand, within Pf6N2E2_915 at 1075.598 kb on + strand at 1075.598 kb on + strand at 1075.598 kb on + strand at 1075.602 kb on + strand at 1075.602 kb on + strand at 1075.603 kb on - strand at 1075.603 kb on - strand at 1075.739 kb on + strand at 1075.740 kb on - strand at 1075.883 kb on + strand at 1075.884 kb on - strand at 1075.884 kb on - strand at 1075.884 kb on - strand at 1075.901 kb on - strand at 1076.024 kb on + strand at 1076.025 kb on - strand at 1076.128 kb on - strand at 1076.202 kb on + strand at 1076.203 kb on - strand at 1076.219 kb on + strand at 1076.219 kb on + strand at 1076.219 kb on + strand at 1076.220 kb on - strand at 1076.220 kb on - strand at 1076.238 kb on + strand at 1076.239 kb on - strand at 1076.390 kb on - strand at 1076.408 kb on - strand at 1076.408 kb on - strand at 1076.419 kb on + strand at 1076.419 kb on + strand at 1076.438 kb on - strand at 1076.438 kb on - strand at 1076.438 kb on - strand at 1076.441 kb on + strand at 1076.441 kb on + strand at 1076.442 kb on - strand at 1076.485 kb on + strand at 1076.549 kb on + strand at 1076.549 kb on + strand at 1076.573 kb on + strand at 1076.646 kb on + strand, within Pf6N2E2_916 at 1076.647 kb on - strand, within Pf6N2E2_916 at 1076.647 kb on - strand, within Pf6N2E2_916 at 1076.736 kb on + strand, within Pf6N2E2_916 at 1076.736 kb on + strand, within Pf6N2E2_916 at 1076.736 kb on + strand, within Pf6N2E2_916 at 1076.736 kb on + strand, within Pf6N2E2_916 at 1076.736 kb on + strand, within Pf6N2E2_916 at 1076.737 kb on - strand, within Pf6N2E2_916 at 1076.801 kb on + strand, within Pf6N2E2_916 at 1076.801 kb on + strand, within Pf6N2E2_916 at 1076.802 kb on - strand, within Pf6N2E2_916 at 1076.848 kb on - strand, within Pf6N2E2_916 at 1076.851 kb on + strand, within Pf6N2E2_916 at 1076.851 kb on + strand, within Pf6N2E2_916 at 1077.039 kb on + strand, within Pf6N2E2_916 at 1077.040 kb on - strand, within Pf6N2E2_916 at 1077.048 kb on + strand, within Pf6N2E2_916 at 1077.049 kb on - strand, within Pf6N2E2_916 at 1077.298 kb on - strand, within Pf6N2E2_916 at 1077.339 kb on + strand, within Pf6N2E2_916 at 1077.340 kb on - strand, within Pf6N2E2_916 at 1077.340 kb on - strand, within Pf6N2E2_916 at 1077.340 kb on - strand, within Pf6N2E2_916 at 1077.340 kb on - strand, within Pf6N2E2_916 at 1077.364 kb on - strand, within Pf6N2E2_916 at 1077.364 kb on - strand, within Pf6N2E2_916 at 1077.445 kb on - strand, within Pf6N2E2_916 at 1077.489 kb on - strand, within Pf6N2E2_916 at 1077.489 kb on - strand, within Pf6N2E2_916 at 1077.646 kb on + strand, within Pf6N2E2_916 at 1077.646 kb on + strand, within Pf6N2E2_916 at 1077.646 kb on + strand, within Pf6N2E2_916 at 1077.647 kb on - strand, within Pf6N2E2_916 at 1077.647 kb on - strand, within Pf6N2E2_916 at 1077.656 kb on - strand, within Pf6N2E2_916 at 1077.784 kb on - strand, within Pf6N2E2_916 at 1077.973 kb on - strand at 1077.984 kb on + strand at 1077.985 kb on - strand at 1078.043 kb on + strand at 1078.043 kb on + strand at 1078.137 kb on + strand at 1078.138 kb on - strand at 1078.151 kb on + strand at 1078.178 kb on + strand at 1078.221 kb on + strand at 1078.252 kb on + strand at 1078.252 kb on + strand at 1078.252 kb on + strand at 1078.255 kb on + strand at 1078.256 kb on - strand at 1078.483 kb on + strand, within Pf6N2E2_917 at 1078.483 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.492 kb on + strand, within Pf6N2E2_917 at 1078.493 kb on - strand, within Pf6N2E2_917 at 1078.493 kb on - strand, within Pf6N2E2_917 at 1078.595 kb on - strand, within Pf6N2E2_917 at 1078.600 kb on + strand, within Pf6N2E2_917 at 1078.600 kb on + strand, within Pf6N2E2_917 at 1078.750 kb on - strand, within Pf6N2E2_917 at 1078.986 kb on + strand, within Pf6N2E2_917 at 1078.986 kb on + strand, within Pf6N2E2_917 at 1078.999 kb on - strand, within Pf6N2E2_917
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,075,474 - Pf6N2E2_915 0.58 -0.8 1,075,598 + +0.9 1,075,598 + -0.4 1,075,598 + -1.9 1,075,602 + +0.9 1,075,602 + +0.9 1,075,603 - +1.9 1,075,603 - -0.6 1,075,739 + -0.3 1,075,740 - +1.6 1,075,883 + +0.3 1,075,884 - -2.1 1,075,884 - -1.6 1,075,884 - -1.3 1,075,901 - -1.4 1,076,024 + -1.3 1,076,025 - +0.3 1,076,128 - +0.5 1,076,202 + +0.0 1,076,203 - +0.7 1,076,219 + -0.9 1,076,219 + +0.0 1,076,219 + -0.1 1,076,220 - -0.3 1,076,220 - -1.0 1,076,238 + +0.2 1,076,239 - -0.2 1,076,390 - +0.3 1,076,408 - +0.8 1,076,408 - +0.3 1,076,419 + +0.4 1,076,419 + +0.0 1,076,438 - -1.3 1,076,438 - +1.3 1,076,438 - -0.6 1,076,441 + -0.4 1,076,441 + +0.9 1,076,442 - -0.1 1,076,485 + +1.2 1,076,549 + +1.8 1,076,549 + -0.3 1,076,573 + -0.2 1,076,646 + Pf6N2E2_916 0.12 +0.1 1,076,647 - Pf6N2E2_916 0.12 +0.6 1,076,647 - Pf6N2E2_916 0.12 +2.5 1,076,736 + Pf6N2E2_916 0.17 +1.3 1,076,736 + Pf6N2E2_916 0.17 +1.3 1,076,736 + Pf6N2E2_916 0.17 +0.8 1,076,736 + Pf6N2E2_916 0.17 +0.7 1,076,736 + Pf6N2E2_916 0.17 +0.9 1,076,737 - Pf6N2E2_916 0.17 +0.2 1,076,801 + Pf6N2E2_916 0.22 +1.2 1,076,801 + Pf6N2E2_916 0.22 +0.6 1,076,802 - Pf6N2E2_916 0.22 +0.9 1,076,848 - Pf6N2E2_916 0.25 +0.8 1,076,851 + Pf6N2E2_916 0.25 +0.7 1,076,851 + Pf6N2E2_916 0.25 -0.5 1,077,039 + Pf6N2E2_916 0.37 +0.4 1,077,040 - Pf6N2E2_916 0.37 -0.5 1,077,048 + Pf6N2E2_916 0.38 -0.3 1,077,049 - Pf6N2E2_916 0.38 -0.3 1,077,298 - Pf6N2E2_916 0.54 +1.4 1,077,339 + Pf6N2E2_916 0.57 +1.0 1,077,340 - Pf6N2E2_916 0.57 +0.4 1,077,340 - Pf6N2E2_916 0.57 +1.3 1,077,340 - Pf6N2E2_916 0.57 +0.1 1,077,340 - Pf6N2E2_916 0.57 -0.8 1,077,364 - Pf6N2E2_916 0.58 +0.9 1,077,364 - Pf6N2E2_916 0.58 +1.9 1,077,445 - Pf6N2E2_916 0.63 +1.0 1,077,489 - Pf6N2E2_916 0.66 -0.5 1,077,489 - Pf6N2E2_916 0.66 +0.5 1,077,646 + Pf6N2E2_916 0.77 +0.9 1,077,646 + Pf6N2E2_916 0.77 +0.7 1,077,646 + Pf6N2E2_916 0.77 +1.2 1,077,647 - Pf6N2E2_916 0.77 -0.7 1,077,647 - Pf6N2E2_916 0.77 -0.8 1,077,656 - Pf6N2E2_916 0.77 +0.4 1,077,784 - Pf6N2E2_916 0.86 +1.8 1,077,973 - +1.1 1,077,984 + +0.7 1,077,985 - +1.1 1,078,043 + +1.3 1,078,043 + +0.3 1,078,137 + -1.0 1,078,138 - +0.8 1,078,151 + +0.9 1,078,178 + -1.4 1,078,221 + +1.3 1,078,252 + +0.4 1,078,252 + +0.7 1,078,252 + -2.3 1,078,255 + +0.4 1,078,256 - -1.0 1,078,483 + Pf6N2E2_917 0.12 -2.0 1,078,483 + Pf6N2E2_917 0.12 +0.6 1,078,492 + Pf6N2E2_917 0.12 +0.7 1,078,492 + Pf6N2E2_917 0.12 -0.3 1,078,492 + Pf6N2E2_917 0.12 +0.4 1,078,492 + Pf6N2E2_917 0.12 -1.2 1,078,492 + Pf6N2E2_917 0.12 +1.3 1,078,492 + Pf6N2E2_917 0.12 -0.9 1,078,492 + Pf6N2E2_917 0.12 +0.4 1,078,493 - Pf6N2E2_917 0.12 +0.7 1,078,493 - Pf6N2E2_917 0.12 +0.3 1,078,595 - Pf6N2E2_917 0.20 -0.4 1,078,600 + Pf6N2E2_917 0.21 +0.2 1,078,600 + Pf6N2E2_917 0.21 +0.7 1,078,750 - Pf6N2E2_917 0.32 -0.1 1,078,986 + Pf6N2E2_917 0.50 -0.5 1,078,986 + Pf6N2E2_917 0.50 -1.0 1,078,999 - Pf6N2E2_917 0.51 +0.7
Or see this region's nucleotide sequence