Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_770 and Pf6N2E2_771 overlap by 4 nucleotides Pf6N2E2_771 and Pf6N2E2_772 are separated by 263 nucleotides Pf6N2E2_772 and Pf6N2E2_773 are separated by 273 nucleotides
Pf6N2E2_770 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 880,977 to 881,501
_770
Pf6N2E2_771 - Ribosyl nicotinamide transporter, PnuC-like, at 881,498 to 882,061
_771
Pf6N2E2_772 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 882,325 to 883,959
_772
Pf6N2E2_773 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 884,233 to 885,063
_773
Position (kb)
882
883
884 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 881.400 kb on + strand, within Pf6N2E2_770 at 881.401 kb on - strand, within Pf6N2E2_770 at 881.404 kb on + strand, within Pf6N2E2_770 at 881.404 kb on + strand, within Pf6N2E2_770 at 881.405 kb on - strand, within Pf6N2E2_770 at 881.405 kb on - strand, within Pf6N2E2_770 at 881.405 kb on - strand, within Pf6N2E2_770 at 881.405 kb on - strand, within Pf6N2E2_770 at 881.568 kb on + strand, within Pf6N2E2_771 at 881.568 kb on + strand, within Pf6N2E2_771 at 881.568 kb on + strand, within Pf6N2E2_771 at 881.569 kb on - strand, within Pf6N2E2_771 at 881.598 kb on + strand, within Pf6N2E2_771 at 881.599 kb on - strand, within Pf6N2E2_771 at 881.877 kb on + strand, within Pf6N2E2_771 at 881.878 kb on - strand, within Pf6N2E2_771 at 881.878 kb on - strand, within Pf6N2E2_771 at 881.896 kb on - strand, within Pf6N2E2_771 at 881.896 kb on - strand, within Pf6N2E2_771 at 881.896 kb on - strand, within Pf6N2E2_771 at 881.922 kb on + strand, within Pf6N2E2_771 at 881.922 kb on + strand, within Pf6N2E2_771 at 881.922 kb on + strand, within Pf6N2E2_771 at 881.952 kb on + strand, within Pf6N2E2_771 at 881.952 kb on + strand, within Pf6N2E2_771 at 881.953 kb on - strand, within Pf6N2E2_771 at 881.953 kb on - strand, within Pf6N2E2_771 at 882.079 kb on + strand at 882.080 kb on - strand at 882.080 kb on - strand at 882.080 kb on - strand at 882.098 kb on + strand at 882.126 kb on - strand at 882.166 kb on + strand at 882.167 kb on - strand at 882.167 kb on - strand at 882.283 kb on + strand at 882.283 kb on + strand at 882.693 kb on + strand, within Pf6N2E2_772 at 882.693 kb on + strand, within Pf6N2E2_772 at 882.694 kb on - strand, within Pf6N2E2_772 at 882.821 kb on - strand, within Pf6N2E2_772 at 882.821 kb on - strand, within Pf6N2E2_772 at 883.022 kb on + strand, within Pf6N2E2_772 at 883.022 kb on + strand, within Pf6N2E2_772 at 883.023 kb on - strand, within Pf6N2E2_772 at 883.427 kb on + strand, within Pf6N2E2_772 at 883.428 kb on - strand, within Pf6N2E2_772 at 883.475 kb on + strand, within Pf6N2E2_772 at 883.475 kb on + strand, within Pf6N2E2_772 at 883.475 kb on + strand, within Pf6N2E2_772 at 883.475 kb on + strand, within Pf6N2E2_772 at 883.476 kb on - strand, within Pf6N2E2_772 at 883.523 kb on + strand, within Pf6N2E2_772 at 883.524 kb on - strand, within Pf6N2E2_772 at 883.524 kb on - strand, within Pf6N2E2_772 at 883.524 kb on - strand, within Pf6N2E2_772 at 883.683 kb on - strand, within Pf6N2E2_772 at 883.697 kb on + strand, within Pf6N2E2_772 at 883.698 kb on - strand, within Pf6N2E2_772 at 883.698 kb on - strand, within Pf6N2E2_772 at 883.712 kb on - strand, within Pf6N2E2_772 at 883.778 kb on + strand, within Pf6N2E2_772 at 883.779 kb on - strand, within Pf6N2E2_772 at 883.781 kb on + strand, within Pf6N2E2_772 at 883.781 kb on + strand, within Pf6N2E2_772 at 883.864 kb on - strand at 883.864 kb on - strand at 883.864 kb on - strand at 883.953 kb on + strand at 883.953 kb on + strand at 883.954 kb on - strand at 883.954 kb on - strand at 883.959 kb on + strand at 883.960 kb on - strand at 883.960 kb on - strand at 883.981 kb on - strand at 883.981 kb on - strand at 883.991 kb on + strand at 883.991 kb on + strand at 884.009 kb on + strand at 884.076 kb on + strand at 884.273 kb on + strand at 884.325 kb on + strand, within Pf6N2E2_773 at 884.325 kb on + strand, within Pf6N2E2_773 at 884.837 kb on - strand, within Pf6N2E2_773 at 884.846 kb on - strand, within Pf6N2E2_773 at 884.846 kb on - strand, within Pf6N2E2_773 at 884.851 kb on + strand, within Pf6N2E2_773
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 881,400 + Pf6N2E2_770 0.81 +0.3 881,401 - Pf6N2E2_770 0.81 +0.2 881,404 + Pf6N2E2_770 0.81 +0.7 881,404 + Pf6N2E2_770 0.81 +0.3 881,405 - Pf6N2E2_770 0.82 -0.7 881,405 - Pf6N2E2_770 0.82 +0.5 881,405 - Pf6N2E2_770 0.82 +0.9 881,405 - Pf6N2E2_770 0.82 +0.9 881,568 + Pf6N2E2_771 0.12 +0.1 881,568 + Pf6N2E2_771 0.12 -0.6 881,568 + Pf6N2E2_771 0.12 -0.6 881,569 - Pf6N2E2_771 0.13 -1.6 881,598 + Pf6N2E2_771 0.18 -0.3 881,599 - Pf6N2E2_771 0.18 +0.7 881,877 + Pf6N2E2_771 0.67 -0.3 881,878 - Pf6N2E2_771 0.67 -2.0 881,878 - Pf6N2E2_771 0.67 -0.8 881,896 - Pf6N2E2_771 0.71 +2.0 881,896 - Pf6N2E2_771 0.71 -0.3 881,896 - Pf6N2E2_771 0.71 +0.1 881,922 + Pf6N2E2_771 0.75 +0.9 881,922 + Pf6N2E2_771 0.75 -0.1 881,922 + Pf6N2E2_771 0.75 +1.0 881,952 + Pf6N2E2_771 0.80 +1.3 881,952 + Pf6N2E2_771 0.80 -0.1 881,953 - Pf6N2E2_771 0.81 +1.0 881,953 - Pf6N2E2_771 0.81 +0.6 882,079 + +0.9 882,080 - -0.1 882,080 - +1.5 882,080 - -0.4 882,098 + +2.0 882,126 - -0.0 882,166 + +0.6 882,167 - +1.6 882,167 - +0.2 882,283 + +1.6 882,283 + +0.1 882,693 + Pf6N2E2_772 0.23 +1.0 882,693 + Pf6N2E2_772 0.23 +0.4 882,694 - Pf6N2E2_772 0.23 +0.3 882,821 - Pf6N2E2_772 0.30 -2.3 882,821 - Pf6N2E2_772 0.30 -0.3 883,022 + Pf6N2E2_772 0.43 +1.1 883,022 + Pf6N2E2_772 0.43 -1.3 883,023 - Pf6N2E2_772 0.43 +0.4 883,427 + Pf6N2E2_772 0.67 +0.1 883,428 - Pf6N2E2_772 0.67 -2.9 883,475 + Pf6N2E2_772 0.70 +0.9 883,475 + Pf6N2E2_772 0.70 +0.5 883,475 + Pf6N2E2_772 0.70 +1.6 883,475 + Pf6N2E2_772 0.70 -0.3 883,476 - Pf6N2E2_772 0.70 +0.1 883,523 + Pf6N2E2_772 0.73 -0.1 883,524 - Pf6N2E2_772 0.73 -0.4 883,524 - Pf6N2E2_772 0.73 +0.5 883,524 - Pf6N2E2_772 0.73 +1.7 883,683 - Pf6N2E2_772 0.83 +0.5 883,697 + Pf6N2E2_772 0.84 -1.3 883,698 - Pf6N2E2_772 0.84 -0.6 883,698 - Pf6N2E2_772 0.84 +1.3 883,712 - Pf6N2E2_772 0.85 +2.0 883,778 + Pf6N2E2_772 0.89 +0.2 883,779 - Pf6N2E2_772 0.89 +2.4 883,781 + Pf6N2E2_772 0.89 +0.4 883,781 + Pf6N2E2_772 0.89 -3.9 883,864 - +1.9 883,864 - +2.1 883,864 - +2.6 883,953 + -2.4 883,953 + -2.0 883,954 - +2.6 883,954 - +3.2 883,959 + -2.8 883,960 - +3.6 883,960 - +0.9 883,981 - +3.2 883,981 - +2.3 883,991 + -2.5 883,991 + -2.8 884,009 + -1.2 884,076 + -1.1 884,273 + +4.6 884,325 + Pf6N2E2_773 0.11 +4.0 884,325 + Pf6N2E2_773 0.11 +4.8 884,837 - Pf6N2E2_773 0.73 +5.4 884,846 - Pf6N2E2_773 0.74 +4.3 884,846 - Pf6N2E2_773 0.74 +4.4 884,851 + Pf6N2E2_773 0.74 +4.7
Or see this region's nucleotide sequence