Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_755 and Pf6N2E2_756 are separated by 373 nucleotides Pf6N2E2_756 and Pf6N2E2_757 are separated by 274 nucleotides
Pf6N2E2_755 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 864,399 to 866,138
_755
Pf6N2E2_756 - hypothetical protein, at 866,512 to 866,685
_756
Pf6N2E2_757 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 866,960 to 867,940
_757
Position (kb)
866
867 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 865.522 kb on + strand, within Pf6N2E2_755 at 865.777 kb on + strand, within Pf6N2E2_755 at 865.777 kb on + strand, within Pf6N2E2_755 at 865.778 kb on - strand, within Pf6N2E2_755 at 865.778 kb on - strand, within Pf6N2E2_755 at 865.778 kb on - strand, within Pf6N2E2_755 at 865.778 kb on - strand, within Pf6N2E2_755 at 865.790 kb on - strand, within Pf6N2E2_755 at 865.859 kb on - strand, within Pf6N2E2_755 at 865.870 kb on + strand, within Pf6N2E2_755 at 865.871 kb on - strand, within Pf6N2E2_755 at 866.057 kb on - strand at 866.211 kb on + strand at 866.211 kb on + strand at 866.211 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.224 kb on + strand at 866.225 kb on - strand at 866.228 kb on - strand at 866.420 kb on + strand at 866.421 kb on - strand at 866.421 kb on - strand at 866.453 kb on + strand at 866.453 kb on + strand at 866.454 kb on - strand at 866.454 kb on - strand at 866.593 kb on + strand, within Pf6N2E2_756 at 866.593 kb on + strand, within Pf6N2E2_756 at 866.594 kb on - strand, within Pf6N2E2_756 at 866.594 kb on - strand, within Pf6N2E2_756 at 866.615 kb on - strand, within Pf6N2E2_756 at 866.645 kb on + strand, within Pf6N2E2_756 at 866.646 kb on - strand, within Pf6N2E2_756 at 866.646 kb on - strand, within Pf6N2E2_756 at 866.646 kb on - strand, within Pf6N2E2_756 at 866.646 kb on - strand, within Pf6N2E2_756 at 866.706 kb on + strand at 866.706 kb on + strand at 866.707 kb on - strand at 866.715 kb on + strand at 866.715 kb on + strand at 866.715 kb on + strand at 866.715 kb on + strand at 866.715 kb on + strand at 866.715 kb on + strand at 866.716 kb on - strand at 866.716 kb on - strand at 866.716 kb on - strand at 866.717 kb on + strand at 866.717 kb on + strand at 866.717 kb on + strand at 866.717 kb on + strand at 866.718 kb on - strand at 866.718 kb on - strand at 866.718 kb on - strand at 866.718 kb on - strand at 866.718 kb on - strand at 866.733 kb on - strand at 866.733 kb on - strand at 866.749 kb on + strand at 866.750 kb on - strand at 866.753 kb on - strand at 866.863 kb on - strand at 866.873 kb on + strand at 866.873 kb on + strand at 866.874 kb on - strand at 866.910 kb on - strand at 866.917 kb on - strand at 866.929 kb on + strand at 866.929 kb on + strand at 866.930 kb on - strand at 866.935 kb on + strand at 866.935 kb on + strand at 866.935 kb on + strand at 866.935 kb on + strand at 866.936 kb on - strand at 866.936 kb on - strand at 866.941 kb on + strand at 866.944 kb on + strand at 866.945 kb on - strand at 867.044 kb on + strand at 867.045 kb on - strand at 867.225 kb on + strand, within Pf6N2E2_757 at 867.225 kb on + strand, within Pf6N2E2_757 at 867.225 kb on + strand, within Pf6N2E2_757 at 867.226 kb on - strand, within Pf6N2E2_757 at 867.226 kb on - strand, within Pf6N2E2_757 at 867.226 kb on - strand, within Pf6N2E2_757 at 867.248 kb on + strand, within Pf6N2E2_757 at 867.248 kb on + strand, within Pf6N2E2_757 at 867.249 kb on - strand, within Pf6N2E2_757 at 867.249 kb on - strand, within Pf6N2E2_757 at 867.251 kb on + strand, within Pf6N2E2_757 at 867.251 kb on + strand, within Pf6N2E2_757 at 867.251 kb on + strand, within Pf6N2E2_757 at 867.252 kb on - strand, within Pf6N2E2_757 at 867.252 kb on - strand, within Pf6N2E2_757 at 867.302 kb on + strand, within Pf6N2E2_757 at 867.302 kb on + strand, within Pf6N2E2_757 at 867.486 kb on - strand, within Pf6N2E2_757 at 867.549 kb on - strand, within Pf6N2E2_757
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 865,522 + Pf6N2E2_755 0.65 -0.5 865,777 + Pf6N2E2_755 0.79 +0.8 865,777 + Pf6N2E2_755 0.79 -0.1 865,778 - Pf6N2E2_755 0.79 +1.6 865,778 - Pf6N2E2_755 0.79 +0.7 865,778 - Pf6N2E2_755 0.79 +0.9 865,778 - Pf6N2E2_755 0.79 +1.1 865,790 - Pf6N2E2_755 0.80 +1.4 865,859 - Pf6N2E2_755 0.84 +1.4 865,870 + Pf6N2E2_755 0.85 +0.6 865,871 - Pf6N2E2_755 0.85 +1.6 866,057 - +0.1 866,211 + -0.1 866,211 + +0.0 866,211 + -1.8 866,224 + -1.3 866,224 + +0.9 866,224 + -0.0 866,224 + +0.6 866,225 - -1.9 866,228 - +0.8 866,420 + -2.2 866,421 - +1.8 866,421 - +2.0 866,453 + +1.3 866,453 + -0.9 866,454 - +0.4 866,454 - +1.1 866,593 + Pf6N2E2_756 0.47 -2.3 866,593 + Pf6N2E2_756 0.47 +0.0 866,594 - Pf6N2E2_756 0.47 +2.3 866,594 - Pf6N2E2_756 0.47 +1.8 866,615 - Pf6N2E2_756 0.59 +1.0 866,645 + Pf6N2E2_756 0.76 -1.9 866,646 - Pf6N2E2_756 0.77 +2.3 866,646 - Pf6N2E2_756 0.77 +1.2 866,646 - Pf6N2E2_756 0.77 +0.9 866,646 - Pf6N2E2_756 0.77 +1.6 866,706 + -1.9 866,706 + -0.4 866,707 - +2.1 866,715 + -0.1 866,715 + -1.5 866,715 + -0.7 866,715 + -2.3 866,715 + -1.1 866,715 + -1.2 866,716 - +0.1 866,716 - +0.5 866,716 - +0.8 866,717 + -1.6 866,717 + -2.1 866,717 + -0.3 866,717 + -1.9 866,718 - +1.9 866,718 - +0.1 866,718 - +1.6 866,718 - +0.7 866,718 - +3.1 866,733 - +2.0 866,733 - +2.3 866,749 + -0.6 866,750 - +0.2 866,753 - +2.2 866,863 - +2.8 866,873 + -0.2 866,873 + -0.3 866,874 - +1.8 866,910 - +2.4 866,917 - +1.1 866,929 + +0.4 866,929 + -2.8 866,930 - +2.0 866,935 + -1.1 866,935 + -0.6 866,935 + -0.3 866,935 + -0.3 866,936 - +1.4 866,936 - +1.2 866,941 + +1.1 866,944 + +0.6 866,945 - +2.2 867,044 + +0.4 867,045 - +0.1 867,225 + Pf6N2E2_757 0.27 +0.9 867,225 + Pf6N2E2_757 0.27 +2.4 867,225 + Pf6N2E2_757 0.27 +1.9 867,226 - Pf6N2E2_757 0.27 -0.9 867,226 - Pf6N2E2_757 0.27 +1.3 867,226 - Pf6N2E2_757 0.27 +1.6 867,248 + Pf6N2E2_757 0.29 +0.3 867,248 + Pf6N2E2_757 0.29 +1.4 867,249 - Pf6N2E2_757 0.29 +1.7 867,249 - Pf6N2E2_757 0.29 +2.4 867,251 + Pf6N2E2_757 0.30 +2.9 867,251 + Pf6N2E2_757 0.30 +1.9 867,251 + Pf6N2E2_757 0.30 +2.7 867,252 - Pf6N2E2_757 0.30 +1.6 867,252 - Pf6N2E2_757 0.30 +2.5 867,302 + Pf6N2E2_757 0.35 +1.4 867,302 + Pf6N2E2_757 0.35 +0.3 867,486 - Pf6N2E2_757 0.54 +0.7 867,549 - Pf6N2E2_757 0.60 +2.0
Or see this region's nucleotide sequence