Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_655 and Pf6N2E2_656 are separated by 150 nucleotides Pf6N2E2_656 and Pf6N2E2_657 are separated by 113 nucleotides Pf6N2E2_657 and Pf6N2E2_658 are separated by 199 nucleotides
Pf6N2E2_655 - Filamentous haemagglutinin family outer membrane protein associated with VreARI signalling system, at 746,494 to 759,111
_655
Pf6N2E2_656 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 759,262 to 761,835
_656
Pf6N2E2_657 - hypothetical protein, at 761,949 to 762,065
_657
Pf6N2E2_658 - sensor histidine kinase, at 762,265 to 763,575
_658
Position (kb) 759
760
761
762 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 758.422 kb on + strand at 758.422 kb on + strand at 758.422 kb on + strand at 758.423 kb on - strand at 758.423 kb on - strand at 758.430 kb on + strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.546 kb on - strand at 758.567 kb on - strand at 759.010 kb on + strand at 759.011 kb on - strand at 759.109 kb on + strand at 759.110 kb on - strand at 759.169 kb on - strand at 759.169 kb on - strand at 759.219 kb on + strand at 759.220 kb on - strand at 759.326 kb on + strand at 759.327 kb on - strand at 759.416 kb on - strand at 759.704 kb on - strand, within Pf6N2E2_656 at 759.704 kb on - strand, within Pf6N2E2_656 at 759.817 kb on - strand, within Pf6N2E2_656 at 759.817 kb on - strand, within Pf6N2E2_656 at 759.959 kb on + strand, within Pf6N2E2_656 at 760.011 kb on - strand, within Pf6N2E2_656 at 760.052 kb on + strand, within Pf6N2E2_656 at 760.053 kb on - strand, within Pf6N2E2_656 at 760.053 kb on - strand, within Pf6N2E2_656 at 760.091 kb on + strand, within Pf6N2E2_656 at 760.092 kb on - strand, within Pf6N2E2_656 at 760.092 kb on - strand, within Pf6N2E2_656 at 760.178 kb on + strand, within Pf6N2E2_656 at 760.179 kb on - strand, within Pf6N2E2_656 at 760.179 kb on - strand, within Pf6N2E2_656 at 760.345 kb on - strand, within Pf6N2E2_656 at 760.638 kb on + strand, within Pf6N2E2_656 at 760.638 kb on + strand, within Pf6N2E2_656 at 760.664 kb on + strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.944 kb on - strand, within Pf6N2E2_656 at 761.300 kb on + strand, within Pf6N2E2_656 at 761.301 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.490 kb on - strand, within Pf6N2E2_656 at 761.495 kb on + strand, within Pf6N2E2_656 at 761.496 kb on - strand, within Pf6N2E2_656 at 761.504 kb on + strand, within Pf6N2E2_656 at 761.534 kb on + strand, within Pf6N2E2_656 at 761.835 kb on + strand at 761.841 kb on + strand at 761.841 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.882 kb on - strand at 761.903 kb on + strand at 761.903 kb on + strand at 761.904 kb on - strand at 761.907 kb on + strand at 761.908 kb on - strand at 762.151 kb on + strand at 762.151 kb on + strand at 762.152 kb on - strand at 762.737 kb on + strand, within Pf6N2E2_658
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 758,422 + -0.9 758,422 + -0.7 758,422 + -0.7 758,423 - -0.0 758,423 - +0.0 758,430 + +0.6 758,487 - +0.0 758,487 - +0.1 758,487 - +0.7 758,487 - -0.2 758,546 - +0.0 758,567 - -0.3 759,010 + +0.4 759,011 - -0.7 759,109 + +0.0 759,110 - -1.8 759,169 - -2.3 759,169 - +0.1 759,219 + +0.6 759,220 - +1.3 759,326 + +0.4 759,327 - +1.0 759,416 - +1.0 759,704 - Pf6N2E2_656 0.17 +0.3 759,704 - Pf6N2E2_656 0.17 +3.6 759,817 - Pf6N2E2_656 0.22 +1.1 759,817 - Pf6N2E2_656 0.22 -0.3 759,959 + Pf6N2E2_656 0.27 +1.4 760,011 - Pf6N2E2_656 0.29 +1.6 760,052 + Pf6N2E2_656 0.31 -1.9 760,053 - Pf6N2E2_656 0.31 +0.3 760,053 - Pf6N2E2_656 0.31 +0.7 760,091 + Pf6N2E2_656 0.32 -4.0 760,092 - Pf6N2E2_656 0.32 +1.3 760,092 - Pf6N2E2_656 0.32 +0.8 760,178 + Pf6N2E2_656 0.36 -0.2 760,179 - Pf6N2E2_656 0.36 -0.5 760,179 - Pf6N2E2_656 0.36 +0.4 760,345 - Pf6N2E2_656 0.42 +1.3 760,638 + Pf6N2E2_656 0.53 +0.4 760,638 + Pf6N2E2_656 0.53 +0.2 760,664 + Pf6N2E2_656 0.54 +1.0 760,827 - Pf6N2E2_656 0.61 +2.5 760,827 - Pf6N2E2_656 0.61 -0.3 760,827 - Pf6N2E2_656 0.61 +1.3 760,827 - Pf6N2E2_656 0.61 -0.1 760,944 - Pf6N2E2_656 0.65 -1.2 761,300 + Pf6N2E2_656 0.79 +0.8 761,301 - Pf6N2E2_656 0.79 +0.0 761,448 - Pf6N2E2_656 0.85 +1.0 761,448 - Pf6N2E2_656 0.85 +1.8 761,490 - Pf6N2E2_656 0.87 +1.9 761,495 + Pf6N2E2_656 0.87 +1.3 761,496 - Pf6N2E2_656 0.87 -0.2 761,504 + Pf6N2E2_656 0.87 -0.4 761,534 + Pf6N2E2_656 0.88 -0.3 761,835 + +0.7 761,841 + -0.6 761,841 + -0.2 761,881 + -0.1 761,881 + +0.3 761,881 + +0.5 761,881 + +1.3 761,881 + +0.4 761,882 - -0.3 761,903 + +1.0 761,903 + +0.2 761,904 - -1.3 761,907 + +0.3 761,908 - -1.0 762,151 + -0.4 762,151 + -0.4 762,152 - +0.8 762,737 + Pf6N2E2_658 0.36 -0.5
Or see this region's nucleotide sequence