Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides Pf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotides Pf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotides
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822
_641
Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801
_642
Position (kb)
732
733 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.976 kb on - strand, within Pf6N2E2_640 at 732.228 kb on + strand at 732.229 kb on - strand at 732.253 kb on - strand at 732.325 kb on + strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.455 kb on - strand, within Pf6N2E2_641 at 732.477 kb on + strand, within Pf6N2E2_641 at 732.622 kb on + strand, within Pf6N2E2_641 at 732.623 kb on - strand, within Pf6N2E2_641 at 732.709 kb on - strand, within Pf6N2E2_641 at 733.517 kb on + strand, within Pf6N2E2_642 at 733.517 kb on + strand, within Pf6N2E2_642 at 733.518 kb on - strand, within Pf6N2E2_642 at 733.518 kb on - strand, within Pf6N2E2_642
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 731,495 + +0.6 731,528 + -1.0 731,528 + -0.0 731,529 - +0.7 731,540 - -0.2 731,540 - +0.3 731,572 + +0.8 731,573 - -0.5 731,573 - +0.3 731,803 + Pf6N2E2_640 0.24 -2.3 731,803 + Pf6N2E2_640 0.24 -1.9 731,804 - Pf6N2E2_640 0.25 -0.3 731,882 + Pf6N2E2_640 0.37 -0.6 731,975 + Pf6N2E2_640 0.52 +0.5 731,975 + Pf6N2E2_640 0.52 -0.3 731,976 - Pf6N2E2_640 0.52 +0.8 732,228 + +2.0 732,229 - +0.6 732,253 - -0.6 732,325 + Pf6N2E2_641 0.14 +0.4 732,326 - Pf6N2E2_641 0.14 +0.4 732,326 - Pf6N2E2_641 0.14 +1.4 732,401 - Pf6N2E2_641 0.27 +0.8 732,401 - Pf6N2E2_641 0.27 +0.3 732,454 + Pf6N2E2_641 0.36 +1.4 732,454 + Pf6N2E2_641 0.36 -1.3 732,454 + Pf6N2E2_641 0.36 +0.7 732,454 + Pf6N2E2_641 0.36 -0.2 732,454 + Pf6N2E2_641 0.36 +0.2 732,455 - Pf6N2E2_641 0.36 +0.3 732,477 + Pf6N2E2_641 0.40 +1.9 732,622 + Pf6N2E2_641 0.65 +0.6 732,623 - Pf6N2E2_641 0.65 -0.1 732,709 - Pf6N2E2_641 0.80 -0.3 733,517 + Pf6N2E2_642 0.70 -0.3 733,517 + Pf6N2E2_642 0.70 -0.9 733,518 - Pf6N2E2_642 0.70 +1.4 733,518 - Pf6N2E2_642 0.70 -0.2
Or see this region's nucleotide sequence