Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_622 and Pf6N2E2_623 overlap by 8 nucleotides Pf6N2E2_623 and Pf6N2E2_624 overlap by 4 nucleotides Pf6N2E2_624 and Pf6N2E2_625 are separated by 9 nucleotides
Pf6N2E2_622 - BatA (Bacteroides aerotolerance operon), at 708,996 to 710,075
_622
Pf6N2E2_623 - FIG00954674: hypothetical protein, at 710,068 to 710,562
_623
Pf6N2E2_624 - hypothetical protein PA3071, at 710,559 to 711,503
_624
Pf6N2E2_625 - MoxR-like ATPase in aerotolerance operon, at 711,513 to 712,472
_625
Position (kb) 710
711
712 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.640 kb on - strand, within Pf6N2E2_622 at 710.168 kb on - strand, within Pf6N2E2_623 at 710.628 kb on - strand at 711.067 kb on + strand, within Pf6N2E2_624 at 711.111 kb on - strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.133 kb on - strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.304 kb on - strand, within Pf6N2E2_624 at 711.304 kb on - strand, within Pf6N2E2_624 at 711.497 kb on + strand at 711.498 kb on - strand at 711.635 kb on + strand, within Pf6N2E2_625 at 711.727 kb on + strand, within Pf6N2E2_625 at 711.799 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.824 kb on - strand, within Pf6N2E2_625 at 712.072 kb on + strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 709,639 + Pf6N2E2_622 0.60 -2.6 709,639 + Pf6N2E2_622 0.60 -2.3 709,640 - Pf6N2E2_622 0.60 -0.6 710,168 - Pf6N2E2_623 0.20 -2.0 710,628 - -3.2 711,067 + Pf6N2E2_624 0.54 -3.1 711,111 - Pf6N2E2_624 0.58 -1.9 711,132 + Pf6N2E2_624 0.61 -2.5 711,132 + Pf6N2E2_624 0.61 -2.9 711,132 + Pf6N2E2_624 0.61 -1.6 711,133 - Pf6N2E2_624 0.61 -4.0 711,303 + Pf6N2E2_624 0.79 -1.7 711,303 + Pf6N2E2_624 0.79 -3.1 711,303 + Pf6N2E2_624 0.79 -2.5 711,303 + Pf6N2E2_624 0.79 -1.3 711,304 - Pf6N2E2_624 0.79 -3.1 711,304 - Pf6N2E2_624 0.79 -4.0 711,497 + -1.3 711,498 - -1.6 711,635 + Pf6N2E2_625 0.13 -3.8 711,727 + Pf6N2E2_625 0.22 -0.5 711,799 + Pf6N2E2_625 0.30 -1.5 711,823 + Pf6N2E2_625 0.32 -3.3 711,823 + Pf6N2E2_625 0.32 -3.5 711,823 + Pf6N2E2_625 0.32 -1.3 711,823 + Pf6N2E2_625 0.32 -3.9 711,824 - Pf6N2E2_625 0.32 -1.9 712,072 + Pf6N2E2_625 0.58 -3.5 712,073 - Pf6N2E2_625 0.58 -3.6 712,073 - Pf6N2E2_625 0.58 -2.9 712,073 - Pf6N2E2_625 0.58 -0.3 712,073 - Pf6N2E2_625 0.58 -2.3 712,073 - Pf6N2E2_625 0.58 -2.3
Or see this region's nucleotide sequence