Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6056 and Pf6N2E2_6057 are separated by 288 nucleotides Pf6N2E2_6057 and Pf6N2E2_6058 overlap by 1 nucleotides
Pf6N2E2_6056 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 6,875,181 to 6,876,524
_6056
Pf6N2E2_6057 - FIG002781: Alpha-L-glutamate ligase family protein, at 6,876,813 to 6,877,799
_6057
Pf6N2E2_6058 - FIG139976: hypothetical protein, at 6,877,799 to 6,879,304
_6058
Position (kb)
6876
6877
6878 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6875.860 kb on + strand, within Pf6N2E2_6056 at 6875.920 kb on + strand, within Pf6N2E2_6056 at 6875.920 kb on + strand, within Pf6N2E2_6056 at 6875.921 kb on - strand, within Pf6N2E2_6056 at 6875.940 kb on - strand, within Pf6N2E2_6056 at 6875.941 kb on + strand, within Pf6N2E2_6056 at 6875.941 kb on + strand, within Pf6N2E2_6056 at 6875.941 kb on + strand, within Pf6N2E2_6056 at 6875.941 kb on + strand, within Pf6N2E2_6056 at 6875.942 kb on - strand, within Pf6N2E2_6056 at 6875.942 kb on - strand, within Pf6N2E2_6056 at 6875.942 kb on - strand, within Pf6N2E2_6056 at 6875.942 kb on - strand, within Pf6N2E2_6056 at 6875.971 kb on + strand, within Pf6N2E2_6056 at 6875.971 kb on + strand, within Pf6N2E2_6056 at 6876.148 kb on + strand, within Pf6N2E2_6056 at 6876.149 kb on - strand, within Pf6N2E2_6056 at 6876.229 kb on + strand, within Pf6N2E2_6056 at 6876.229 kb on + strand, within Pf6N2E2_6056 at 6876.230 kb on - strand, within Pf6N2E2_6056 at 6876.230 kb on - strand, within Pf6N2E2_6056 at 6876.230 kb on - strand, within Pf6N2E2_6056 at 6876.283 kb on - strand, within Pf6N2E2_6056 at 6876.297 kb on + strand, within Pf6N2E2_6056 at 6876.297 kb on + strand, within Pf6N2E2_6056 at 6876.298 kb on - strand, within Pf6N2E2_6056 at 6876.298 kb on - strand, within Pf6N2E2_6056 at 6876.472 kb on + strand at 6876.507 kb on + strand at 6876.507 kb on + strand at 6876.508 kb on - strand at 6876.508 kb on - strand at 6876.564 kb on + strand at 6876.564 kb on + strand at 6876.564 kb on + strand at 6876.565 kb on - strand at 6876.588 kb on - strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.608 kb on + strand at 6876.609 kb on - strand at 6876.609 kb on - strand at 6876.612 kb on + strand at 6876.612 kb on + strand at 6876.612 kb on + strand at 6876.612 kb on + strand at 6876.612 kb on + strand at 6876.613 kb on - strand at 6876.834 kb on - strand at 6877.046 kb on - strand, within Pf6N2E2_6057 at 6877.066 kb on + strand at 6877.066 kb on + strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.711 kb on + strand at 6877.711 kb on + strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.723 kb on + strand at 6877.723 kb on + strand at 6877.733 kb on + strand at 6877.926 kb on + strand at 6877.926 kb on + strand at 6877.927 kb on - strand at 6878.622 kb on + strand, within Pf6N2E2_6058 at 6878.623 kb on - strand, within Pf6N2E2_6058
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,875,860 + Pf6N2E2_6056 0.51 +0.5 6,875,920 + Pf6N2E2_6056 0.55 +0.2 6,875,920 + Pf6N2E2_6056 0.55 -0.4 6,875,921 - Pf6N2E2_6056 0.55 +0.4 6,875,940 - Pf6N2E2_6056 0.56 -0.6 6,875,941 + Pf6N2E2_6056 0.57 +3.0 6,875,941 + Pf6N2E2_6056 0.57 -0.3 6,875,941 + Pf6N2E2_6056 0.57 -0.5 6,875,941 + Pf6N2E2_6056 0.57 +2.0 6,875,942 - Pf6N2E2_6056 0.57 -1.7 6,875,942 - Pf6N2E2_6056 0.57 -0.2 6,875,942 - Pf6N2E2_6056 0.57 +0.2 6,875,942 - Pf6N2E2_6056 0.57 +0.7 6,875,971 + Pf6N2E2_6056 0.59 -0.5 6,875,971 + Pf6N2E2_6056 0.59 +0.2 6,876,148 + Pf6N2E2_6056 0.72 +0.6 6,876,149 - Pf6N2E2_6056 0.72 +0.3 6,876,229 + Pf6N2E2_6056 0.78 -0.0 6,876,229 + Pf6N2E2_6056 0.78 -0.4 6,876,230 - Pf6N2E2_6056 0.78 +0.1 6,876,230 - Pf6N2E2_6056 0.78 -0.1 6,876,230 - Pf6N2E2_6056 0.78 +0.9 6,876,283 - Pf6N2E2_6056 0.82 -1.4 6,876,297 + Pf6N2E2_6056 0.83 -0.6 6,876,297 + Pf6N2E2_6056 0.83 -1.4 6,876,298 - Pf6N2E2_6056 0.83 +0.6 6,876,298 - Pf6N2E2_6056 0.83 -1.7 6,876,472 + +0.1 6,876,507 + +0.5 6,876,507 + +0.3 6,876,508 - -0.3 6,876,508 - +0.8 6,876,564 + -0.3 6,876,564 + -0.3 6,876,564 + -0.6 6,876,565 - +0.9 6,876,588 - -0.6 6,876,608 + -0.4 6,876,608 + -0.2 6,876,608 + -0.1 6,876,608 + -0.2 6,876,608 + +1.0 6,876,608 + +2.3 6,876,608 + -2.2 6,876,608 + +0.2 6,876,608 + +0.3 6,876,608 + +1.0 6,876,609 - -0.4 6,876,609 - +0.4 6,876,612 + +0.0 6,876,612 + -1.5 6,876,612 + +0.6 6,876,612 + +1.1 6,876,612 + +0.6 6,876,613 - +0.1 6,876,834 - -0.9 6,877,046 - Pf6N2E2_6057 0.24 -1.9 6,877,066 + -1.1 6,877,066 + Pf6N2E2_6057 0.26 -1.9 6,877,067 - Pf6N2E2_6057 0.26 -3.4 6,877,067 - Pf6N2E2_6057 0.26 -2.3 6,877,067 - Pf6N2E2_6057 0.26 -1.1 6,877,711 + -1.9 6,877,711 + -3.4 6,877,712 - -0.1 6,877,712 - -2.3 6,877,712 - -2.4 6,877,712 - -2.9 6,877,723 + -2.4 6,877,723 + -1.1 6,877,733 + -3.5 6,877,926 + -2.3 6,877,926 + -2.9 6,877,927 - -1.7 6,878,622 + Pf6N2E2_6058 0.55 -3.7 6,878,623 - Pf6N2E2_6058 0.55 -3.9
Or see this region's nucleotide sequence