Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5918 and Pf6N2E2_5919 are separated by 22 nucleotides Pf6N2E2_5919 and Pf6N2E2_5920 overlap by 4 nucleotides
Pf6N2E2_5918 - hypothetical protein, at 6,737,805 to 6,738,074
_5918
Pf6N2E2_5919 - Flp pilus assembly protein TadD, contains TPR repeats, at 6,738,097 to 6,738,870
_5919
Pf6N2E2_5920 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 6,738,867 to 6,740,090
_5920
Position (kb)
6738
6739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6737.118 kb on + strand at 6737.119 kb on - strand at 6737.119 kb on - strand at 6737.122 kb on + strand at 6737.122 kb on + strand at 6737.123 kb on - strand at 6737.123 kb on - strand at 6737.158 kb on - strand at 6737.280 kb on + strand at 6737.281 kb on - strand at 6737.281 kb on - strand at 6737.281 kb on - strand at 6737.283 kb on + strand at 6737.308 kb on - strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.440 kb on + strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.441 kb on - strand at 6737.585 kb on + strand at 6737.585 kb on + strand at 6737.585 kb on + strand at 6737.647 kb on - strand at 6737.706 kb on + strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.707 kb on - strand at 6737.764 kb on + strand at 6737.765 kb on - strand at 6737.765 kb on - strand at 6737.765 kb on - strand at 6738.091 kb on - strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.099 kb on - strand at 6738.099 kb on - strand at 6738.276 kb on - strand, within Pf6N2E2_5919 at 6738.384 kb on - strand, within Pf6N2E2_5919 at 6738.449 kb on + strand, within Pf6N2E2_5919 at 6738.653 kb on + strand, within Pf6N2E2_5919 at 6738.654 kb on - strand, within Pf6N2E2_5919 at 6738.815 kb on + strand at 6738.816 kb on - strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.917 kb on - strand at 6738.917 kb on - strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.940 kb on + strand at 6738.941 kb on - strand at 6739.343 kb on - strand, within Pf6N2E2_5920 at 6739.457 kb on + strand, within Pf6N2E2_5920 at 6739.459 kb on + strand, within Pf6N2E2_5920 at 6739.505 kb on + strand, within Pf6N2E2_5920 at 6739.506 kb on - strand, within Pf6N2E2_5920 at 6739.722 kb on + strand, within Pf6N2E2_5920 at 6739.723 kb on - strand, within Pf6N2E2_5920 at 6739.789 kb on - strand, within Pf6N2E2_5920
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,737,118 + +0.3 6,737,119 - -0.7 6,737,119 - +0.4 6,737,122 + -0.3 6,737,122 + +0.1 6,737,123 - +0.0 6,737,123 - +0.4 6,737,158 - -0.9 6,737,280 + +0.9 6,737,281 - -0.1 6,737,281 - -0.2 6,737,281 - +1.1 6,737,283 + +0.6 6,737,308 - +0.0 6,737,440 + +0.4 6,737,440 + -0.4 6,737,440 + +1.5 6,737,440 + +0.2 6,737,441 - +1.1 6,737,441 - +1.7 6,737,441 - -0.2 6,737,441 - +0.9 6,737,441 - -0.1 6,737,441 - -0.1 6,737,441 - +0.4 6,737,441 - -1.1 6,737,441 - +0.7 6,737,441 - +0.3 6,737,585 + +1.1 6,737,585 + -2.4 6,737,585 + +1.2 6,737,647 - +0.6 6,737,706 + -0.2 6,737,707 - -0.6 6,737,707 - +0.4 6,737,707 - +0.1 6,737,707 - +0.3 6,737,707 - +0.1 6,737,764 + -2.0 6,737,765 - -1.5 6,737,765 - -0.4 6,737,765 - +2.2 6,738,091 - -3.3 6,738,098 + -0.1 6,738,098 + +0.6 6,738,098 + -0.7 6,738,098 + +0.5 6,738,098 + +0.2 6,738,099 - -3.1 6,738,099 - -2.6 6,738,276 - Pf6N2E2_5919 0.23 -2.1 6,738,384 - Pf6N2E2_5919 0.37 -2.5 6,738,449 + Pf6N2E2_5919 0.45 +0.4 6,738,653 + Pf6N2E2_5919 0.72 +0.3 6,738,654 - Pf6N2E2_5919 0.72 -1.0 6,738,815 + +0.4 6,738,816 - -1.5 6,738,916 + -0.4 6,738,916 + -1.2 6,738,916 + -0.5 6,738,916 + -0.3 6,738,916 + +0.7 6,738,917 - -1.7 6,738,917 - -0.2 6,738,920 + +0.6 6,738,920 + +0.7 6,738,920 + +0.4 6,738,920 + +0.6 6,738,920 + +0.1 6,738,920 + -0.7 6,738,920 + -0.4 6,738,920 + +0.3 6,738,920 + -1.1 6,738,921 - -2.1 6,738,921 - +0.2 6,738,921 - +0.6 6,738,921 - -2.3 6,738,921 - -2.5 6,738,921 - -0.5 6,738,921 - +1.3 6,738,921 - -1.0 6,738,921 - -1.0 6,738,921 - +0.3 6,738,940 + -0.8 6,738,941 - +0.9 6,739,343 - Pf6N2E2_5920 0.39 -0.1 6,739,457 + Pf6N2E2_5920 0.48 +0.8 6,739,459 + Pf6N2E2_5920 0.48 +0.2 6,739,505 + Pf6N2E2_5920 0.52 -2.5 6,739,506 - Pf6N2E2_5920 0.52 +0.3 6,739,722 + Pf6N2E2_5920 0.70 -1.2 6,739,723 - Pf6N2E2_5920 0.70 -1.6 6,739,789 - Pf6N2E2_5920 0.75 +0.9
Or see this region's nucleotide sequence