Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_588 and Pf6N2E2_589 are separated by 89 nucleotides Pf6N2E2_589 and Pf6N2E2_590 are separated by 201 nucleotides Pf6N2E2_590 and Pf6N2E2_591 are separated by 327 nucleotides Pf6N2E2_591 and Pf6N2E2_592 overlap by 35 nucleotides
Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613
_588
Pf6N2E2_589 - Ribonuclease HI (EC 3.1.26.4), at 666,703 to 667,155
_589
Pf6N2E2_590 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 667,357 to 668,118
_590
Pf6N2E2_591 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 668,446 to 669,060
_591
Pf6N2E2_592 - Transcriptional regulators, LysR family, at 669,026 to 669,919
_592
Position (kb) 667
668
669 Strain fitness (log2 ratio) -2
-1
0
1
2 at 666.489 kb on - strand, within Pf6N2E2_588 at 666.611 kb on + strand at 666.694 kb on + strand at 666.694 kb on + strand at 666.716 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand at 668.128 kb on - strand at 668.128 kb on - strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.155 kb on - strand at 668.155 kb on - strand at 668.155 kb on - strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.201 kb on - strand at 668.285 kb on - strand at 668.329 kb on + strand at 668.329 kb on + strand at 668.330 kb on - strand at 668.330 kb on - strand at 668.330 kb on - strand at 668.445 kb on + strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.515 kb on + strand, within Pf6N2E2_591 at 668.515 kb on + strand, within Pf6N2E2_591 at 668.516 kb on - strand, within Pf6N2E2_591 at 668.517 kb on + strand, within Pf6N2E2_591 at 668.517 kb on + strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.626 kb on + strand, within Pf6N2E2_591 at 668.626 kb on + strand, within Pf6N2E2_591 at 668.627 kb on - strand, within Pf6N2E2_591 at 668.692 kb on + strand, within Pf6N2E2_591 at 668.693 kb on - strand, within Pf6N2E2_591 at 668.693 kb on - strand, within Pf6N2E2_591 at 668.917 kb on + strand, within Pf6N2E2_591 at 668.918 kb on - strand, within Pf6N2E2_591 at 668.968 kb on - strand, within Pf6N2E2_591
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 666,489 - Pf6N2E2_588 0.84 +0.8 666,611 + +0.6 666,694 + -0.4 666,694 + -1.8 666,716 + -0.1 667,156 + +0.7 667,156 + +0.8 667,156 + +1.6 668,128 - +0.4 668,128 - +0.4 668,154 + -0.4 668,154 + +1.6 668,154 + -0.7 668,154 + -0.2 668,155 - +0.1 668,155 - +0.6 668,155 - +0.0 668,158 + -0.9 668,158 + -1.5 668,158 + +1.2 668,158 + +0.0 668,158 + -0.2 668,159 - +0.4 668,159 - +0.3 668,159 - +0.9 668,159 - +0.4 668,159 - +0.3 668,159 - +1.3 668,201 - -0.6 668,285 - +0.7 668,329 + +2.0 668,329 + +0.6 668,330 - -0.5 668,330 - -0.7 668,330 - -0.2 668,445 + -0.8 668,446 - +1.1 668,446 - -0.3 668,446 - -0.5 668,446 - -0.4 668,515 + Pf6N2E2_591 0.11 -0.4 668,515 + Pf6N2E2_591 0.11 -0.5 668,516 - Pf6N2E2_591 0.11 -0.4 668,517 + Pf6N2E2_591 0.12 -1.9 668,517 + Pf6N2E2_591 0.12 +1.4 668,518 - Pf6N2E2_591 0.12 +0.1 668,518 - Pf6N2E2_591 0.12 +0.2 668,518 - Pf6N2E2_591 0.12 +1.0 668,518 - Pf6N2E2_591 0.12 -0.0 668,518 - Pf6N2E2_591 0.12 -2.1 668,518 - Pf6N2E2_591 0.12 +1.2 668,626 + Pf6N2E2_591 0.29 +0.7 668,626 + Pf6N2E2_591 0.29 -1.3 668,627 - Pf6N2E2_591 0.29 -1.5 668,692 + Pf6N2E2_591 0.40 +0.8 668,693 - Pf6N2E2_591 0.40 +0.6 668,693 - Pf6N2E2_591 0.40 +0.0 668,917 + Pf6N2E2_591 0.77 -0.7 668,918 - Pf6N2E2_591 0.77 -0.3 668,968 - Pf6N2E2_591 0.85 +1.0
Or see this region's nucleotide sequence