Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5844 and Pf6N2E2_5845 are separated by 134 nucleotides Pf6N2E2_5845 and Pf6N2E2_5846 are separated by 25 nucleotides Pf6N2E2_5846 and Pf6N2E2_5847 are separated by 422 nucleotides
Pf6N2E2_5844 - Membrane protein, putative, at 6,659,730 to 6,660,644
_5844
Pf6N2E2_5845 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 6,660,779 to 6,661,534
_5845
Pf6N2E2_5846 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 6,661,560 to 6,662,858
_5846
Pf6N2E2_5847 - Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline- binding/permease protein, at 6,663,281 to 6,664,135
_5847
Position (kb)
6661
6662
6663 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6660.642 kb on + strand at 6660.642 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.709 kb on + strand at 6660.710 kb on - strand at 6660.780 kb on + strand at 6660.780 kb on + strand at 6660.814 kb on - strand at 6660.819 kb on + strand at 6660.819 kb on + strand at 6660.820 kb on - strand at 6660.820 kb on - strand at 6660.834 kb on + strand at 6660.949 kb on - strand, within Pf6N2E2_5845 at 6660.949 kb on - strand, within Pf6N2E2_5845 at 6660.973 kb on - strand, within Pf6N2E2_5845 at 6661.029 kb on - strand, within Pf6N2E2_5845 at 6661.029 kb on - strand, within Pf6N2E2_5845 at 6661.035 kb on + strand, within Pf6N2E2_5845 at 6661.036 kb on - strand, within Pf6N2E2_5845 at 6661.056 kb on + strand, within Pf6N2E2_5845 at 6661.056 kb on + strand, within Pf6N2E2_5845 at 6661.344 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.377 kb on + strand, within Pf6N2E2_5845 at 6661.378 kb on - strand, within Pf6N2E2_5845 at 6661.378 kb on - strand, within Pf6N2E2_5845 at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.557 kb on + strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.558 kb on - strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.607 kb on + strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.608 kb on - strand at 6661.747 kb on + strand, within Pf6N2E2_5846 at 6661.748 kb on - strand, within Pf6N2E2_5846 at 6661.762 kb on + strand, within Pf6N2E2_5846 at 6661.846 kb on + strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6661.847 kb on - strand, within Pf6N2E2_5846 at 6662.057 kb on - strand, within Pf6N2E2_5846 at 6662.083 kb on + strand, within Pf6N2E2_5846 at 6662.101 kb on + strand, within Pf6N2E2_5846 at 6662.102 kb on - strand, within Pf6N2E2_5846 at 6662.122 kb on - strand, within Pf6N2E2_5846 at 6662.131 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.203 kb on + strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.204 kb on - strand, within Pf6N2E2_5846 at 6662.320 kb on + strand, within Pf6N2E2_5846 at 6662.321 kb on - strand, within Pf6N2E2_5846 at 6662.323 kb on + strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.324 kb on - strand, within Pf6N2E2_5846 at 6662.377 kb on + strand, within Pf6N2E2_5846 at 6662.377 kb on + strand, within Pf6N2E2_5846 at 6662.378 kb on - strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.491 kb on + strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.492 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.495 kb on - strand, within Pf6N2E2_5846 at 6662.509 kb on + strand, within Pf6N2E2_5846 at 6662.510 kb on - strand, within Pf6N2E2_5846 at 6662.510 kb on - strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.522 kb on + strand, within Pf6N2E2_5846 at 6662.523 kb on - strand, within Pf6N2E2_5846 at 6662.770 kb on + strand at 6662.770 kb on + strand at 6662.770 kb on + strand at 6662.771 kb on - strand at 6662.771 kb on - strand at 6662.771 kb on - strand at 6662.817 kb on - strand at 6662.871 kb on + strand at 6662.872 kb on - strand at 6662.872 kb on - strand at 6662.902 kb on + strand at 6662.903 kb on - strand at 6662.965 kb on + strand at 6662.968 kb on + strand at 6663.004 kb on - strand at 6663.008 kb on - strand at 6663.008 kb on - strand at 6663.035 kb on - strand at 6663.056 kb on + strand at 6663.056 kb on + strand at 6663.057 kb on - strand at 6663.192 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.222 kb on + strand at 6663.223 kb on - strand at 6663.223 kb on - strand at 6663.223 kb on - strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.226 kb on + strand at 6663.227 kb on - strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.228 kb on + strand at 6663.229 kb on - strand at 6663.229 kb on - strand at 6663.229 kb on - strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.276 kb on + strand at 6663.277 kb on - strand at 6663.449 kb on + strand, within Pf6N2E2_5847 at 6663.557 kb on + strand, within Pf6N2E2_5847 at 6663.591 kb on - strand, within Pf6N2E2_5847 at 6663.638 kb on + strand, within Pf6N2E2_5847 at 6663.756 kb on - strand, within Pf6N2E2_5847 at 6663.756 kb on - strand, within Pf6N2E2_5847
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,660,642 + -2.0 6,660,642 + -0.1 6,660,709 + -0.8 6,660,709 + -1.5 6,660,709 + -0.6 6,660,709 + -1.6 6,660,709 + -1.0 6,660,709 + -0.8 6,660,709 + -0.8 6,660,709 + +0.6 6,660,710 - -0.3 6,660,780 + -2.1 6,660,780 + -1.0 6,660,814 - -1.1 6,660,819 + -3.1 6,660,819 + -3.4 6,660,820 - -2.7 6,660,820 - -3.5 6,660,834 + -1.7 6,660,949 - Pf6N2E2_5845 0.22 -1.3 6,660,949 - Pf6N2E2_5845 0.22 -2.1 6,660,973 - Pf6N2E2_5845 0.26 -3.0 6,661,029 - Pf6N2E2_5845 0.33 -0.5 6,661,029 - Pf6N2E2_5845 0.33 -0.8 6,661,035 + Pf6N2E2_5845 0.34 -1.7 6,661,036 - Pf6N2E2_5845 0.34 -0.9 6,661,056 + Pf6N2E2_5845 0.37 -4.0 6,661,056 + Pf6N2E2_5845 0.37 -0.1 6,661,344 + Pf6N2E2_5845 0.75 -2.8 6,661,377 + Pf6N2E2_5845 0.79 -3.2 6,661,377 + Pf6N2E2_5845 0.79 -1.8 6,661,377 + Pf6N2E2_5845 0.79 -1.1 6,661,378 - Pf6N2E2_5845 0.79 -2.4 6,661,378 - Pf6N2E2_5845 0.79 -3.5 6,661,557 + -0.8 6,661,557 + -2.3 6,661,557 + -0.7 6,661,557 + -2.4 6,661,557 + +0.4 6,661,558 - -0.4 6,661,558 - -3.6 6,661,558 - -1.2 6,661,558 - -0.3 6,661,558 - -0.9 6,661,558 - -3.0 6,661,558 - -4.1 6,661,607 + -2.3 6,661,607 + -3.1 6,661,607 + -2.7 6,661,607 + -1.1 6,661,607 + -2.2 6,661,608 - -2.4 6,661,608 - -1.1 6,661,608 - -4.1 6,661,608 - -3.1 6,661,608 - -1.9 6,661,747 + Pf6N2E2_5846 0.14 -2.7 6,661,748 - Pf6N2E2_5846 0.14 -2.6 6,661,762 + Pf6N2E2_5846 0.16 -1.7 6,661,846 + Pf6N2E2_5846 0.22 -3.1 6,661,847 - Pf6N2E2_5846 0.22 -2.7 6,661,847 - Pf6N2E2_5846 0.22 -3.2 6,661,847 - Pf6N2E2_5846 0.22 -1.9 6,661,847 - Pf6N2E2_5846 0.22 -1.7 6,662,057 - Pf6N2E2_5846 0.38 -3.2 6,662,083 + Pf6N2E2_5846 0.40 -1.9 6,662,101 + Pf6N2E2_5846 0.42 -1.7 6,662,102 - Pf6N2E2_5846 0.42 -2.1 6,662,122 - Pf6N2E2_5846 0.43 -3.1 6,662,131 + Pf6N2E2_5846 0.44 -2.4 6,662,203 + Pf6N2E2_5846 0.49 -1.9 6,662,203 + Pf6N2E2_5846 0.49 -3.2 6,662,203 + Pf6N2E2_5846 0.49 +1.5 6,662,204 - Pf6N2E2_5846 0.50 -3.1 6,662,204 - Pf6N2E2_5846 0.50 -1.1 6,662,204 - Pf6N2E2_5846 0.50 -2.1 6,662,320 + Pf6N2E2_5846 0.59 -2.4 6,662,321 - Pf6N2E2_5846 0.59 -3.2 6,662,323 + Pf6N2E2_5846 0.59 -3.4 6,662,324 - Pf6N2E2_5846 0.59 -2.1 6,662,324 - Pf6N2E2_5846 0.59 -2.5 6,662,324 - Pf6N2E2_5846 0.59 -3.9 6,662,377 + Pf6N2E2_5846 0.63 -2.3 6,662,377 + Pf6N2E2_5846 0.63 -2.2 6,662,378 - Pf6N2E2_5846 0.63 -4.0 6,662,491 + Pf6N2E2_5846 0.72 -1.5 6,662,491 + Pf6N2E2_5846 0.72 -0.1 6,662,491 + Pf6N2E2_5846 0.72 -2.6 6,662,491 + Pf6N2E2_5846 0.72 -3.6 6,662,492 - Pf6N2E2_5846 0.72 -1.3 6,662,492 - Pf6N2E2_5846 0.72 -2.1 6,662,492 - Pf6N2E2_5846 0.72 -1.9 6,662,492 - Pf6N2E2_5846 0.72 -1.7 6,662,492 - Pf6N2E2_5846 0.72 -1.3 6,662,492 - Pf6N2E2_5846 0.72 +0.7 6,662,495 - Pf6N2E2_5846 0.72 -2.9 6,662,495 - Pf6N2E2_5846 0.72 -4.3 6,662,495 - Pf6N2E2_5846 0.72 -1.9 6,662,495 - Pf6N2E2_5846 0.72 -2.9 6,662,509 + Pf6N2E2_5846 0.73 -3.2 6,662,510 - Pf6N2E2_5846 0.73 -2.0 6,662,510 - Pf6N2E2_5846 0.73 -0.9 6,662,522 + Pf6N2E2_5846 0.74 -0.7 6,662,522 + Pf6N2E2_5846 0.74 -2.0 6,662,522 + Pf6N2E2_5846 0.74 -2.4 6,662,522 + Pf6N2E2_5846 0.74 -0.8 6,662,522 + Pf6N2E2_5846 0.74 -1.2 6,662,522 + Pf6N2E2_5846 0.74 -3.0 6,662,522 + Pf6N2E2_5846 0.74 -2.7 6,662,522 + Pf6N2E2_5846 0.74 -3.5 6,662,523 - Pf6N2E2_5846 0.74 -2.7 6,662,770 + -2.7 6,662,770 + -3.2 6,662,770 + -1.5 6,662,771 - +0.5 6,662,771 - -2.5 6,662,771 - -2.2 6,662,817 - -4.5 6,662,871 + -3.1 6,662,872 - +1.0 6,662,872 - +0.2 6,662,902 + -2.1 6,662,903 - +1.2 6,662,965 + +0.3 6,662,968 + -1.9 6,663,004 - +1.5 6,663,008 - +1.5 6,663,008 - +2.0 6,663,035 - +2.9 6,663,056 + -0.9 6,663,056 + +0.7 6,663,057 - +2.3 6,663,192 + -0.1 6,663,222 + +2.9 6,663,222 + -0.4 6,663,222 + -0.1 6,663,222 + +0.5 6,663,222 + -1.3 6,663,222 + -0.1 6,663,222 + +0.6 6,663,222 + -1.0 6,663,223 - +0.8 6,663,223 - +2.3 6,663,223 - +0.8 6,663,226 + +0.7 6,663,226 + +0.7 6,663,226 + -0.2 6,663,226 + -0.7 6,663,226 + -2.1 6,663,226 + +1.1 6,663,227 - +1.3 6,663,228 + +2.1 6,663,228 + +1.7 6,663,228 + +0.5 6,663,228 + +0.5 6,663,228 + -1.1 6,663,228 + +0.1 6,663,229 - +2.4 6,663,229 - +1.4 6,663,229 - +1.8 6,663,276 + -2.0 6,663,276 + -0.6 6,663,276 + +0.6 6,663,276 + -0.3 6,663,276 + +1.1 6,663,277 - +2.0 6,663,449 + Pf6N2E2_5847 0.20 -1.1 6,663,557 + Pf6N2E2_5847 0.32 -1.0 6,663,591 - Pf6N2E2_5847 0.36 -0.7 6,663,638 + Pf6N2E2_5847 0.42 -0.0 6,663,756 - Pf6N2E2_5847 0.56 +1.3 6,663,756 - Pf6N2E2_5847 0.56 -0.4
Or see this region's nucleotide sequence