Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6159 and Pf6N2E2_6160 are separated by 75 nucleotides Pf6N2E2_6160 and Pf6N2E2_5678 are separated by 43 nucleotides Pf6N2E2_5678 and Pf6N2E2_5679 are separated by 160 nucleotides Pf6N2E2_5679 and Pf6N2E2_5680 are separated by 21 nucleotides
Pf6N2E2_6159 - tRNA-Arg-ACG, at 6,491,920 to 6,491,993
_6159
Pf6N2E2_6160 - tRNA-Arg-ACG, at 6,492,069 to 6,492,142
_6160
Pf6N2E2_5678 - hypothetical protein, at 6,492,186 to 6,492,692
_5678
Pf6N2E2_5679 - hypothetical protein, at 6,492,853 to 6,492,993
_5679
Pf6N2E2_5680 - Mg/Co/Ni transporter MgtE / CBS domain, at 6,493,015 to 6,494,457
_5680
Position (kb)
6492
6493 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6491.999 kb on + strand at 6491.999 kb on + strand at 6492.024 kb on + strand at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.256 kb on + strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.257 kb on - strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.260 kb on + strand, within Pf6N2E2_5678 at 6492.261 kb on - strand, within Pf6N2E2_5678 at 6492.262 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.318 kb on + strand, within Pf6N2E2_5678 at 6492.552 kb on + strand, within Pf6N2E2_5678 at 6492.648 kb on + strand at 6492.649 kb on - strand at 6492.729 kb on - strand at 6492.729 kb on - strand at 6492.854 kb on + strand at 6492.871 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.908 kb on + strand, within Pf6N2E2_5679 at 6492.910 kb on + strand, within Pf6N2E2_5679 at 6492.910 kb on + strand, within Pf6N2E2_5679 at 6492.922 kb on + strand, within Pf6N2E2_5679 at 6492.929 kb on + strand, within Pf6N2E2_5679 at 6492.929 kb on + strand, within Pf6N2E2_5679 at 6492.933 kb on + strand at 6492.943 kb on + strand, within Pf6N2E2_5679 at 6492.943 kb on + strand, within Pf6N2E2_5679 at 6492.964 kb on + strand, within Pf6N2E2_5679 at 6493.014 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,491,999 + -0.7 6,491,999 + -0.6 6,492,024 + -0.2 6,492,256 + Pf6N2E2_5678 0.14 -1.0 6,492,256 + Pf6N2E2_5678 0.14 +0.2 6,492,256 + Pf6N2E2_5678 0.14 -0.2 6,492,256 + Pf6N2E2_5678 0.14 -0.2 6,492,256 + Pf6N2E2_5678 0.14 +0.1 6,492,256 + Pf6N2E2_5678 0.14 +0.6 6,492,256 + Pf6N2E2_5678 0.14 -0.0 6,492,256 + Pf6N2E2_5678 0.14 -1.0 6,492,256 + Pf6N2E2_5678 0.14 -1.2 6,492,257 - Pf6N2E2_5678 0.14 +1.3 6,492,257 - Pf6N2E2_5678 0.14 +0.2 6,492,257 - Pf6N2E2_5678 0.14 +0.1 6,492,257 - Pf6N2E2_5678 0.14 +1.2 6,492,257 - Pf6N2E2_5678 0.14 +1.0 6,492,257 - Pf6N2E2_5678 0.14 +1.0 6,492,257 - Pf6N2E2_5678 0.14 +0.6 6,492,260 + Pf6N2E2_5678 0.15 +0.6 6,492,260 + Pf6N2E2_5678 0.15 +1.1 6,492,260 + Pf6N2E2_5678 0.15 +0.3 6,492,261 - Pf6N2E2_5678 0.15 +0.9 6,492,262 + Pf6N2E2_5678 0.15 +1.2 6,492,318 + Pf6N2E2_5678 0.26 -2.0 6,492,318 + Pf6N2E2_5678 0.26 +0.0 6,492,318 + Pf6N2E2_5678 0.26 -0.6 6,492,318 + Pf6N2E2_5678 0.26 -0.9 6,492,552 + Pf6N2E2_5678 0.72 -0.5 6,492,648 + -0.8 6,492,649 - -0.8 6,492,729 - +0.1 6,492,729 - +1.6 6,492,854 + +0.2 6,492,871 + Pf6N2E2_5679 0.13 -1.2 6,492,908 + Pf6N2E2_5679 0.39 -2.1 6,492,908 + Pf6N2E2_5679 0.39 -1.3 6,492,908 + Pf6N2E2_5679 0.39 -1.9 6,492,910 + Pf6N2E2_5679 0.40 -1.0 6,492,910 + Pf6N2E2_5679 0.40 -1.7 6,492,922 + Pf6N2E2_5679 0.49 -0.3 6,492,929 + Pf6N2E2_5679 0.54 -1.6 6,492,929 + Pf6N2E2_5679 0.54 -0.5 6,492,933 + -0.0 6,492,943 + Pf6N2E2_5679 0.64 -1.1 6,492,943 + Pf6N2E2_5679 0.64 -1.8 6,492,964 + Pf6N2E2_5679 0.79 -1.6 6,493,014 + +2.0
Or see this region's nucleotide sequence