Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5614 and Pf6N2E2_5615 are separated by 13 nucleotides Pf6N2E2_5615 and Pf6N2E2_5616 are separated by 155 nucleotides
Pf6N2E2_5614 - RND multidrug efflux transporter; Acriflavin resistance protein, at 6,428,166 to 6,431,192
_5614
Pf6N2E2_5615 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 6,431,206 to 6,432,354
_5615
Pf6N2E2_5616 - outer membrane component of uptake system, probably for ferrous iron (from data) , at 6,432,510 to 6,433,607
_5616
Position (kb)
6431
6432
6433 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6430.234 kb on + strand, within Pf6N2E2_5614 at 6430.235 kb on - strand, within Pf6N2E2_5614 at 6430.296 kb on + strand, within Pf6N2E2_5614 at 6430.297 kb on - strand, within Pf6N2E2_5614 at 6430.297 kb on - strand, within Pf6N2E2_5614 at 6430.297 kb on - strand, within Pf6N2E2_5614 at 6430.347 kb on + strand, within Pf6N2E2_5614 at 6430.347 kb on + strand, within Pf6N2E2_5614 at 6430.347 kb on + strand, within Pf6N2E2_5614 at 6430.347 kb on + strand, within Pf6N2E2_5614 at 6430.348 kb on - strand, within Pf6N2E2_5614 at 6430.348 kb on - strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.351 kb on + strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.352 kb on - strand, within Pf6N2E2_5614 at 6430.390 kb on + strand, within Pf6N2E2_5614 at 6430.390 kb on + strand, within Pf6N2E2_5614 at 6430.390 kb on + strand, within Pf6N2E2_5614 at 6430.391 kb on - strand, within Pf6N2E2_5614 at 6430.525 kb on + strand, within Pf6N2E2_5614 at 6430.525 kb on + strand, within Pf6N2E2_5614 at 6430.525 kb on + strand, within Pf6N2E2_5614 at 6430.526 kb on - strand, within Pf6N2E2_5614 at 6430.526 kb on - strand, within Pf6N2E2_5614 at 6430.526 kb on - strand, within Pf6N2E2_5614 at 6430.585 kb on - strand, within Pf6N2E2_5614 at 6430.726 kb on + strand, within Pf6N2E2_5614 at 6430.726 kb on + strand, within Pf6N2E2_5614 at 6430.726 kb on + strand, within Pf6N2E2_5614 at 6430.726 kb on + strand, within Pf6N2E2_5614 at 6430.774 kb on + strand, within Pf6N2E2_5614 at 6430.775 kb on - strand, within Pf6N2E2_5614 at 6430.775 kb on - strand, within Pf6N2E2_5614 at 6430.775 kb on - strand, within Pf6N2E2_5614 at 6430.775 kb on - strand, within Pf6N2E2_5614 at 6430.775 kb on - strand, within Pf6N2E2_5614 at 6430.913 kb on - strand at 6430.957 kb on + strand at 6430.957 kb on + strand at 6430.958 kb on - strand at 6430.958 kb on - strand at 6430.958 kb on - strand at 6430.958 kb on - strand at 6430.958 kb on - strand at 6430.958 kb on - strand at 6431.008 kb on + strand at 6431.008 kb on + strand at 6431.008 kb on + strand at 6431.008 kb on + strand at 6431.008 kb on + strand at 6431.009 kb on - strand at 6431.009 kb on - strand at 6431.084 kb on - strand at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.459 kb on + strand, within Pf6N2E2_5615 at 6431.460 kb on - strand, within Pf6N2E2_5615 at 6431.460 kb on - strand, within Pf6N2E2_5615 at 6431.460 kb on - strand, within Pf6N2E2_5615 at 6431.484 kb on - strand, within Pf6N2E2_5615 at 6431.737 kb on + strand, within Pf6N2E2_5615 at 6431.737 kb on + strand, within Pf6N2E2_5615 at 6431.737 kb on + strand, within Pf6N2E2_5615 at 6431.737 kb on + strand, within Pf6N2E2_5615 at 6431.738 kb on - strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.741 kb on + strand, within Pf6N2E2_5615 at 6431.742 kb on - strand, within Pf6N2E2_5615 at 6431.742 kb on - strand, within Pf6N2E2_5615 at 6431.742 kb on - strand, within Pf6N2E2_5615 at 6431.742 kb on - strand, within Pf6N2E2_5615 at 6431.753 kb on + strand, within Pf6N2E2_5615 at 6431.753 kb on + strand, within Pf6N2E2_5615 at 6431.753 kb on + strand, within Pf6N2E2_5615 at 6431.753 kb on + strand, within Pf6N2E2_5615 at 6431.753 kb on + strand, within Pf6N2E2_5615 at 6431.754 kb on - strand, within Pf6N2E2_5615 at 6431.754 kb on - strand, within Pf6N2E2_5615 at 6431.804 kb on + strand, within Pf6N2E2_5615 at 6431.805 kb on - strand, within Pf6N2E2_5615 at 6432.269 kb on + strand at 6432.269 kb on + strand at 6432.269 kb on + strand at 6432.269 kb on + strand at 6432.270 kb on - strand at 6432.270 kb on - strand at 6432.270 kb on - strand at 6432.388 kb on + strand at 6432.389 kb on - strand at 6432.389 kb on - strand at 6432.394 kb on + strand at 6432.394 kb on + strand at 6432.394 kb on + strand at 6432.394 kb on + strand at 6432.394 kb on + strand at 6432.395 kb on - strand at 6432.408 kb on - strand at 6432.425 kb on + strand at 6432.592 kb on + strand at 6432.593 kb on - strand at 6432.593 kb on - strand at 6432.593 kb on - strand at 6432.784 kb on + strand, within Pf6N2E2_5616 at 6432.785 kb on - strand, within Pf6N2E2_5616 at 6432.785 kb on - strand, within Pf6N2E2_5616 at 6433.235 kb on - strand, within Pf6N2E2_5616 at 6433.235 kb on - strand, within Pf6N2E2_5616 at 6433.253 kb on + strand, within Pf6N2E2_5616 at 6433.254 kb on - strand, within Pf6N2E2_5616 at 6433.273 kb on + strand, within Pf6N2E2_5616 at 6433.274 kb on - strand, within Pf6N2E2_5616 at 6433.339 kb on + strand, within Pf6N2E2_5616 at 6433.339 kb on + strand, within Pf6N2E2_5616 at 6433.340 kb on - strand, within Pf6N2E2_5616
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,430,234 + Pf6N2E2_5614 0.68 +1.2 6,430,235 - Pf6N2E2_5614 0.68 +0.2 6,430,296 + Pf6N2E2_5614 0.70 +0.0 6,430,297 - Pf6N2E2_5614 0.70 -1.6 6,430,297 - Pf6N2E2_5614 0.70 -0.2 6,430,297 - Pf6N2E2_5614 0.70 +0.7 6,430,347 + Pf6N2E2_5614 0.72 +0.7 6,430,347 + Pf6N2E2_5614 0.72 -1.0 6,430,347 + Pf6N2E2_5614 0.72 -1.3 6,430,347 + Pf6N2E2_5614 0.72 -1.2 6,430,348 - Pf6N2E2_5614 0.72 -0.3 6,430,348 - Pf6N2E2_5614 0.72 +0.2 6,430,351 + Pf6N2E2_5614 0.72 -0.7 6,430,351 + Pf6N2E2_5614 0.72 -0.2 6,430,351 + Pf6N2E2_5614 0.72 -3.8 6,430,351 + Pf6N2E2_5614 0.72 -0.4 6,430,351 + Pf6N2E2_5614 0.72 -0.4 6,430,351 + Pf6N2E2_5614 0.72 -0.2 6,430,352 - Pf6N2E2_5614 0.72 +0.8 6,430,352 - Pf6N2E2_5614 0.72 -0.3 6,430,352 - Pf6N2E2_5614 0.72 -0.6 6,430,352 - Pf6N2E2_5614 0.72 +0.0 6,430,352 - Pf6N2E2_5614 0.72 +0.5 6,430,352 - Pf6N2E2_5614 0.72 -2.0 6,430,352 - Pf6N2E2_5614 0.72 -1.6 6,430,352 - Pf6N2E2_5614 0.72 +0.1 6,430,352 - Pf6N2E2_5614 0.72 +0.4 6,430,390 + Pf6N2E2_5614 0.73 +0.4 6,430,390 + Pf6N2E2_5614 0.73 +1.0 6,430,390 + Pf6N2E2_5614 0.73 +0.3 6,430,391 - Pf6N2E2_5614 0.74 +0.3 6,430,525 + Pf6N2E2_5614 0.78 -0.2 6,430,525 + Pf6N2E2_5614 0.78 -0.3 6,430,525 + Pf6N2E2_5614 0.78 +0.6 6,430,526 - Pf6N2E2_5614 0.78 +1.2 6,430,526 - Pf6N2E2_5614 0.78 -0.6 6,430,526 - Pf6N2E2_5614 0.78 +0.4 6,430,585 - Pf6N2E2_5614 0.80 -1.0 6,430,726 + Pf6N2E2_5614 0.85 +1.0 6,430,726 + Pf6N2E2_5614 0.85 +0.4 6,430,726 + Pf6N2E2_5614 0.85 -1.2 6,430,726 + Pf6N2E2_5614 0.85 +0.0 6,430,774 + Pf6N2E2_5614 0.86 -0.1 6,430,775 - Pf6N2E2_5614 0.86 +1.3 6,430,775 - Pf6N2E2_5614 0.86 -0.7 6,430,775 - Pf6N2E2_5614 0.86 +0.6 6,430,775 - Pf6N2E2_5614 0.86 +0.9 6,430,775 - Pf6N2E2_5614 0.86 -0.9 6,430,913 - -2.7 6,430,957 + -2.0 6,430,957 + -0.2 6,430,958 - -1.4 6,430,958 - -2.0 6,430,958 - +0.8 6,430,958 - -0.5 6,430,958 - +0.0 6,430,958 - +0.1 6,431,008 + +0.4 6,431,008 + -0.8 6,431,008 + -1.6 6,431,008 + -0.1 6,431,008 + -1.6 6,431,009 - +1.4 6,431,009 - +1.5 6,431,084 - -0.2 6,431,459 + Pf6N2E2_5615 0.22 -0.4 6,431,459 + Pf6N2E2_5615 0.22 -0.2 6,431,459 + Pf6N2E2_5615 0.22 +0.6 6,431,459 + Pf6N2E2_5615 0.22 +1.1 6,431,459 + Pf6N2E2_5615 0.22 -0.6 6,431,459 + Pf6N2E2_5615 0.22 +1.3 6,431,459 + Pf6N2E2_5615 0.22 +0.5 6,431,460 - Pf6N2E2_5615 0.22 -1.0 6,431,460 - Pf6N2E2_5615 0.22 -0.6 6,431,460 - Pf6N2E2_5615 0.22 -0.2 6,431,484 - Pf6N2E2_5615 0.24 -0.1 6,431,737 + Pf6N2E2_5615 0.46 -0.8 6,431,737 + Pf6N2E2_5615 0.46 +0.7 6,431,737 + Pf6N2E2_5615 0.46 -2.0 6,431,737 + Pf6N2E2_5615 0.46 -0.4 6,431,738 - Pf6N2E2_5615 0.46 -1.0 6,431,741 + Pf6N2E2_5615 0.47 +0.7 6,431,741 + Pf6N2E2_5615 0.47 -1.6 6,431,741 + Pf6N2E2_5615 0.47 +0.6 6,431,741 + Pf6N2E2_5615 0.47 -1.4 6,431,741 + Pf6N2E2_5615 0.47 -0.7 6,431,741 + Pf6N2E2_5615 0.47 -0.1 6,431,741 + Pf6N2E2_5615 0.47 +0.2 6,431,741 + Pf6N2E2_5615 0.47 +1.2 6,431,741 + Pf6N2E2_5615 0.47 -0.8 6,431,742 - Pf6N2E2_5615 0.47 -0.5 6,431,742 - Pf6N2E2_5615 0.47 -0.8 6,431,742 - Pf6N2E2_5615 0.47 +0.9 6,431,742 - Pf6N2E2_5615 0.47 +0.0 6,431,753 + Pf6N2E2_5615 0.48 -1.3 6,431,753 + Pf6N2E2_5615 0.48 -1.4 6,431,753 + Pf6N2E2_5615 0.48 -0.9 6,431,753 + Pf6N2E2_5615 0.48 -0.8 6,431,753 + Pf6N2E2_5615 0.48 +0.0 6,431,754 - Pf6N2E2_5615 0.48 +0.1 6,431,754 - Pf6N2E2_5615 0.48 -0.4 6,431,804 + Pf6N2E2_5615 0.52 -0.9 6,431,805 - Pf6N2E2_5615 0.52 -0.1 6,432,269 + -1.2 6,432,269 + +0.4 6,432,269 + -1.2 6,432,269 + +0.3 6,432,270 - -0.2 6,432,270 - +0.0 6,432,270 - +2.0 6,432,388 + -0.4 6,432,389 - -0.3 6,432,389 - +0.5 6,432,394 + +0.8 6,432,394 + -1.6 6,432,394 + -0.2 6,432,394 + +0.3 6,432,394 + -0.9 6,432,395 - +1.0 6,432,408 - +0.2 6,432,425 + -0.6 6,432,592 + +0.2 6,432,593 - +0.7 6,432,593 - +0.2 6,432,593 - +1.9 6,432,784 + Pf6N2E2_5616 0.25 +0.7 6,432,785 - Pf6N2E2_5616 0.25 +0.4 6,432,785 - Pf6N2E2_5616 0.25 +0.8 6,433,235 - Pf6N2E2_5616 0.66 -0.6 6,433,235 - Pf6N2E2_5616 0.66 -0.8 6,433,253 + Pf6N2E2_5616 0.68 -1.2 6,433,254 - Pf6N2E2_5616 0.68 -0.7 6,433,273 + Pf6N2E2_5616 0.69 -0.9 6,433,274 - Pf6N2E2_5616 0.70 -0.3 6,433,339 + Pf6N2E2_5616 0.76 +0.7 6,433,339 + Pf6N2E2_5616 0.76 -0.9 6,433,340 - Pf6N2E2_5616 0.76 -0.2
Or see this region's nucleotide sequence