Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5544 and Pf6N2E2_5545 are separated by 166 nucleotides Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides Pf6N2E2_5546 and Pf6N2E2_6157 are separated by 63 nucleotides Pf6N2E2_6157 and Pf6N2E2_5547 are separated by 152 nucleotides
Pf6N2E2_5544 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 6,358,942 to 6,359,706
_5544
Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520
_5545
Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214
_5546
Pf6N2E2_6157 - tRNA-Lys-TTT, at 6,361,278 to 6,361,350
_6157
Pf6N2E2_5547 - hypothetical protein, at 6,361,503 to 6,362,933
_5547
Position (kb)
6360
6361
6362 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.543 kb on - strand, within Pf6N2E2_5544 at 6359.575 kb on + strand, within Pf6N2E2_5544 at 6359.591 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.621 kb on + strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.622 kb on - strand, within Pf6N2E2_5544 at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.704 kb on + strand at 6359.705 kb on - strand at 6359.705 kb on - strand at 6359.777 kb on - strand at 6359.850 kb on + strand at 6359.857 kb on + strand at 6359.858 kb on - strand at 6359.873 kb on - strand at 6359.949 kb on + strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.950 kb on - strand, within Pf6N2E2_5545 at 6359.987 kb on + strand, within Pf6N2E2_5545 at 6360.063 kb on - strand, within Pf6N2E2_5545 at 6360.074 kb on + strand, within Pf6N2E2_5545 at 6360.075 kb on - strand, within Pf6N2E2_5545 at 6360.188 kb on - strand, within Pf6N2E2_5545 at 6360.362 kb on + strand, within Pf6N2E2_5545 at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.487 kb on + strand at 6360.488 kb on - strand at 6360.488 kb on - strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.491 kb on + strand at 6360.492 kb on - strand at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.841 kb on + strand, within Pf6N2E2_5546 at 6360.842 kb on - strand, within Pf6N2E2_5546 at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.425 kb on - strand at 6361.668 kb on - strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.918 kb on + strand, within Pf6N2E2_5547 at 6361.919 kb on - strand, within Pf6N2E2_5547 at 6361.950 kb on + strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.175 kb on + strand, within Pf6N2E2_5547
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,359,543 - Pf6N2E2_5544 0.79 -2.8 6,359,543 - Pf6N2E2_5544 0.79 -0.8 6,359,543 - Pf6N2E2_5544 0.79 +0.6 6,359,543 - Pf6N2E2_5544 0.79 -0.8 6,359,575 + Pf6N2E2_5544 0.83 -0.6 6,359,591 + Pf6N2E2_5544 0.85 -3.4 6,359,621 + Pf6N2E2_5544 0.89 -0.7 6,359,621 + Pf6N2E2_5544 0.89 -0.7 6,359,621 + Pf6N2E2_5544 0.89 -0.9 6,359,621 + Pf6N2E2_5544 0.89 -0.3 6,359,622 - Pf6N2E2_5544 0.89 -0.6 6,359,622 - Pf6N2E2_5544 0.89 +0.5 6,359,622 - Pf6N2E2_5544 0.89 -1.2 6,359,704 + -0.0 6,359,704 + +1.1 6,359,704 + -0.0 6,359,704 + -0.4 6,359,705 - +0.1 6,359,705 - +0.9 6,359,777 - +2.4 6,359,850 + +0.9 6,359,857 + -0.9 6,359,858 - +1.4 6,359,873 - +3.1 6,359,949 + Pf6N2E2_5545 0.12 +2.5 6,359,950 - Pf6N2E2_5545 0.12 +1.5 6,359,950 - Pf6N2E2_5545 0.12 +2.4 6,359,987 + Pf6N2E2_5545 0.18 +2.1 6,360,063 - Pf6N2E2_5545 0.29 +2.2 6,360,074 + Pf6N2E2_5545 0.31 +2.6 6,360,075 - Pf6N2E2_5545 0.31 +2.0 6,360,188 - Pf6N2E2_5545 0.49 +2.4 6,360,362 + Pf6N2E2_5545 0.75 +2.8 6,360,487 + +2.9 6,360,487 + +1.5 6,360,487 + +3.2 6,360,488 - +2.5 6,360,488 - +2.1 6,360,491 + +2.6 6,360,491 + +1.5 6,360,491 + +2.8 6,360,492 - +1.3 6,360,521 + +2.9 6,360,521 + +1.3 6,360,522 - +1.9 6,360,522 - +1.5 6,360,631 - Pf6N2E2_5546 0.16 +1.9 6,360,631 - Pf6N2E2_5546 0.16 +2.3 6,360,631 - Pf6N2E2_5546 0.16 +1.4 6,360,649 - Pf6N2E2_5546 0.18 +2.4 6,360,819 + Pf6N2E2_5546 0.43 +1.6 6,360,819 + Pf6N2E2_5546 0.43 +0.8 6,360,819 + Pf6N2E2_5546 0.43 +1.6 6,360,819 + Pf6N2E2_5546 0.43 +2.8 6,360,819 + Pf6N2E2_5546 0.43 +2.5 6,360,819 + Pf6N2E2_5546 0.43 +2.8 6,360,819 + Pf6N2E2_5546 0.43 +2.1 6,360,819 + Pf6N2E2_5546 0.43 +1.9 6,360,819 + Pf6N2E2_5546 0.43 +2.1 6,360,819 + Pf6N2E2_5546 0.43 +1.9 6,360,819 + Pf6N2E2_5546 0.43 +1.8 6,360,820 - Pf6N2E2_5546 0.43 +2.2 6,360,820 - Pf6N2E2_5546 0.43 +2.5 6,360,820 - Pf6N2E2_5546 0.43 +1.7 6,360,820 - Pf6N2E2_5546 0.43 +2.7 6,360,820 - Pf6N2E2_5546 0.43 +1.9 6,360,820 - Pf6N2E2_5546 0.43 +1.8 6,360,833 + Pf6N2E2_5546 0.45 +2.3 6,360,833 + Pf6N2E2_5546 0.45 +1.8 6,360,841 + Pf6N2E2_5546 0.46 +1.5 6,360,842 - Pf6N2E2_5546 0.46 +1.9 6,361,424 + +1.1 6,361,424 + -0.5 6,361,424 + +0.6 6,361,425 - -1.5 6,361,668 - Pf6N2E2_5547 0.12 -0.1 6,361,746 + Pf6N2E2_5547 0.17 -0.3 6,361,746 + Pf6N2E2_5547 0.17 +1.6 6,361,752 + Pf6N2E2_5547 0.17 +0.8 6,361,752 + Pf6N2E2_5547 0.17 -2.3 6,361,752 + Pf6N2E2_5547 0.17 +0.6 6,361,752 + Pf6N2E2_5547 0.17 +1.5 6,361,752 + Pf6N2E2_5547 0.17 -0.2 6,361,753 - Pf6N2E2_5547 0.17 -0.4 6,361,753 - Pf6N2E2_5547 0.17 +0.0 6,361,753 - Pf6N2E2_5547 0.17 +0.9 6,361,753 - Pf6N2E2_5547 0.17 -0.2 6,361,753 - Pf6N2E2_5547 0.17 -0.5 6,361,753 - Pf6N2E2_5547 0.17 -0.8 6,361,769 - Pf6N2E2_5547 0.19 +0.3 6,361,769 - Pf6N2E2_5547 0.19 +0.5 6,361,851 + Pf6N2E2_5547 0.24 -0.1 6,361,851 + Pf6N2E2_5547 0.24 +0.0 6,361,851 + Pf6N2E2_5547 0.24 +0.9 6,361,851 + Pf6N2E2_5547 0.24 -1.0 6,361,852 - Pf6N2E2_5547 0.24 +0.2 6,361,852 - Pf6N2E2_5547 0.24 +0.3 6,361,918 + Pf6N2E2_5547 0.29 +0.0 6,361,919 - Pf6N2E2_5547 0.29 +0.4 6,361,950 + Pf6N2E2_5547 0.31 -0.6 6,361,951 - Pf6N2E2_5547 0.31 -0.7 6,361,951 - Pf6N2E2_5547 0.31 +2.9 6,361,951 - Pf6N2E2_5547 0.31 +0.6 6,362,008 + Pf6N2E2_5547 0.35 -1.1 6,362,008 + Pf6N2E2_5547 0.35 -2.0 6,362,009 - Pf6N2E2_5547 0.35 -0.7 6,362,009 - Pf6N2E2_5547 0.35 -0.1 6,362,009 - Pf6N2E2_5547 0.35 -2.0 6,362,009 - Pf6N2E2_5547 0.35 -0.7 6,362,009 - Pf6N2E2_5547 0.35 +1.0 6,362,034 + Pf6N2E2_5547 0.37 -0.6 6,362,034 + Pf6N2E2_5547 0.37 +0.8 6,362,035 - Pf6N2E2_5547 0.37 -3.3 6,362,035 - Pf6N2E2_5547 0.37 -0.5 6,362,035 - Pf6N2E2_5547 0.37 -2.4 6,362,089 + Pf6N2E2_5547 0.41 +0.3 6,362,089 + Pf6N2E2_5547 0.41 -1.0 6,362,090 - Pf6N2E2_5547 0.41 +0.8 6,362,090 - Pf6N2E2_5547 0.41 +2.0 6,362,122 + Pf6N2E2_5547 0.43 +0.1 6,362,122 + Pf6N2E2_5547 0.43 -0.3 6,362,122 + Pf6N2E2_5547 0.43 -0.6 6,362,122 + Pf6N2E2_5547 0.43 -1.1 6,362,122 + Pf6N2E2_5547 0.43 -1.7 6,362,122 + Pf6N2E2_5547 0.43 +1.6 6,362,123 - Pf6N2E2_5547 0.43 +0.6 6,362,123 - Pf6N2E2_5547 0.43 -0.2 6,362,123 - Pf6N2E2_5547 0.43 -0.8 6,362,123 - Pf6N2E2_5547 0.43 +1.3 6,362,123 - Pf6N2E2_5547 0.43 +0.0 6,362,123 - Pf6N2E2_5547 0.43 -0.6 6,362,123 - Pf6N2E2_5547 0.43 -0.2 6,362,123 - Pf6N2E2_5547 0.43 +0.0 6,362,130 + Pf6N2E2_5547 0.44 +0.1 6,362,130 + Pf6N2E2_5547 0.44 +0.3 6,362,130 + Pf6N2E2_5547 0.44 +1.6 6,362,130 + Pf6N2E2_5547 0.44 +0.4 6,362,131 - Pf6N2E2_5547 0.44 +0.0 6,362,131 - Pf6N2E2_5547 0.44 -1.7 6,362,131 - Pf6N2E2_5547 0.44 -0.4 6,362,175 + Pf6N2E2_5547 0.47 -0.5
Or see this region's nucleotide sequence