Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5535 and Pf6N2E2_5536 are separated by 113 nucleotides Pf6N2E2_5536 and Pf6N2E2_5537 are separated by 0 nucleotides Pf6N2E2_5537 and Pf6N2E2_5538 are separated by 127 nucleotides Pf6N2E2_5538 and Pf6N2E2_5539 overlap by 11 nucleotides
Pf6N2E2_5535 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 6,351,872 to 6,352,396
_5535
Pf6N2E2_5536 - Holliday junction DNA helicase RuvA, at 6,352,510 to 6,353,118
_5536
Pf6N2E2_5537 - Holliday junction DNA helicase RuvB, at 6,353,119 to 6,354,180
_5537
Pf6N2E2_5538 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 6,354,308 to 6,354,775
_5538
Pf6N2E2_5539 - MotA/TolQ/ExbB proton channel family protein, at 6,354,765 to 6,355,460
_5539
Position (kb)
6353
6354
6355 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6352.572 kb on - strand, within Pf6N2E2_5536 at 6353.215 kb on + strand at 6354.052 kb on + strand, within Pf6N2E2_5537 at 6354.113 kb on + strand at 6354.114 kb on - strand at 6354.149 kb on + strand at 6354.295 kb on + strand at 6354.295 kb on + strand at 6354.302 kb on + strand at 6354.302 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.412 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.739 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,352,572 - Pf6N2E2_5536 0.10 -0.4 6,353,215 + -1.8 6,354,052 + Pf6N2E2_5537 0.88 -2.3 6,354,113 + -2.2 6,354,114 - -3.4 6,354,149 + -0.3 6,354,295 + -0.2 6,354,295 + +0.5 6,354,302 + +0.7 6,354,302 + -0.7 6,354,307 + +0.3 6,354,307 + +1.0 6,354,307 + +0.7 6,354,356 + Pf6N2E2_5538 0.10 +0.6 6,354,356 + Pf6N2E2_5538 0.10 -0.4 6,354,359 + Pf6N2E2_5538 0.11 -1.8 6,354,359 + Pf6N2E2_5538 0.11 -0.0 6,354,389 + Pf6N2E2_5538 0.17 +0.7 6,354,389 + Pf6N2E2_5538 0.17 +0.1 6,354,389 + Pf6N2E2_5538 0.17 +1.6 6,354,392 + Pf6N2E2_5538 0.18 +0.7 6,354,392 + Pf6N2E2_5538 0.18 +0.1 6,354,392 + Pf6N2E2_5538 0.18 -0.2 6,354,392 + Pf6N2E2_5538 0.18 +0.1 6,354,401 + Pf6N2E2_5538 0.20 +1.2 6,354,401 + Pf6N2E2_5538 0.20 +0.5 6,354,412 + Pf6N2E2_5538 0.22 -0.1 6,354,552 + Pf6N2E2_5538 0.52 -0.1 6,354,552 + Pf6N2E2_5538 0.52 +0.6 6,354,673 + Pf6N2E2_5538 0.78 -0.1 6,354,673 + Pf6N2E2_5538 0.78 +1.1 6,354,705 + Pf6N2E2_5538 0.85 -0.1 6,354,705 + Pf6N2E2_5538 0.85 +0.9 6,354,739 + +0.2
Or see this region's nucleotide sequence