Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5489 and Pf6N2E2_5490 are separated by 39 nucleotides Pf6N2E2_5490 and Pf6N2E2_5491 are separated by 236 nucleotides Pf6N2E2_5491 and Pf6N2E2_5492 overlap by 8 nucleotides Pf6N2E2_5492 and Pf6N2E2_5493 are separated by 28 nucleotides
Pf6N2E2_5489 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 6,307,738 to 6,309,261
_5489
Pf6N2E2_5490 - hypothetical protein, at 6,309,301 to 6,309,501
_5490
Pf6N2E2_5491 - FOG: GGDEF domain, at 6,309,738 to 6,310,844
_5491
Pf6N2E2_5492 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 6,310,837 to 6,311,772
_5492
Pf6N2E2_5493 - Enoyl-CoA hydratase (EC 4.2.1.17), at 6,311,801 to 6,312,550
_5493
Position (kb)
6309
6310
6311 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6309.298 kb on - strand at 6309.298 kb on - strand at 6309.342 kb on + strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.345 kb on - strand, within Pf6N2E2_5490 at 6309.345 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.661 kb on - strand at 6309.671 kb on - strand at 6309.671 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.747 kb on - strand at 6309.798 kb on + strand at 6309.798 kb on + strand at 6309.799 kb on - strand at 6309.799 kb on - strand at 6309.817 kb on + strand at 6309.817 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.054 kb on + strand, within Pf6N2E2_5491 at 6310.054 kb on + strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.139 kb on + strand, within Pf6N2E2_5491 at 6310.140 kb on - strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.143 kb on - strand, within Pf6N2E2_5491 at 6310.143 kb on - strand, within Pf6N2E2_5491 at 6310.158 kb on + strand, within Pf6N2E2_5491 at 6310.159 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491 at 6310.567 kb on + strand, within Pf6N2E2_5491 at 6310.567 kb on + strand, within Pf6N2E2_5491 at 6310.567 kb on + strand, within Pf6N2E2_5491 at 6310.567 kb on + strand, within Pf6N2E2_5491 at 6310.568 kb on - strand, within Pf6N2E2_5491 at 6310.568 kb on - strand, within Pf6N2E2_5491 at 6310.568 kb on - strand, within Pf6N2E2_5491 at 6310.568 kb on - strand, within Pf6N2E2_5491 at 6310.578 kb on + strand, within Pf6N2E2_5491 at 6310.888 kb on - strand at 6310.930 kb on + strand at 6310.930 kb on + strand at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.390 kb on - strand, within Pf6N2E2_5492 at 6311.390 kb on - strand, within Pf6N2E2_5492 at 6311.509 kb on - strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.667 kb on - strand, within Pf6N2E2_5492 at 6311.713 kb on + strand at 6311.713 kb on + strand at 6311.714 kb on - strand at 6311.714 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,309,298 - +1.2 6,309,298 - -0.1 6,309,342 + Pf6N2E2_5490 0.20 -0.9 6,309,343 - Pf6N2E2_5490 0.21 +0.6 6,309,343 - Pf6N2E2_5490 0.21 -0.3 6,309,343 - Pf6N2E2_5490 0.21 +0.8 6,309,343 - Pf6N2E2_5490 0.21 -0.8 6,309,343 - Pf6N2E2_5490 0.21 +1.4 6,309,343 - Pf6N2E2_5490 0.21 -2.2 6,309,343 - Pf6N2E2_5490 0.21 -1.8 6,309,345 - Pf6N2E2_5490 0.22 +0.6 6,309,345 - Pf6N2E2_5490 0.22 -1.4 6,309,391 - Pf6N2E2_5490 0.45 +0.2 6,309,391 - Pf6N2E2_5490 0.45 +0.3 6,309,391 - Pf6N2E2_5490 0.45 +0.9 6,309,391 - Pf6N2E2_5490 0.45 -0.6 6,309,391 - Pf6N2E2_5490 0.45 +0.3 6,309,391 - Pf6N2E2_5490 0.45 +0.1 6,309,391 - Pf6N2E2_5490 0.45 -0.6 6,309,391 - Pf6N2E2_5490 0.45 +1.2 6,309,391 - Pf6N2E2_5490 0.45 +0.7 6,309,661 - +1.0 6,309,671 - +0.3 6,309,671 - +1.8 6,309,695 - +0.6 6,309,695 - +1.7 6,309,695 - +1.2 6,309,695 - +0.6 6,309,747 - +1.7 6,309,798 + +1.3 6,309,798 + -0.3 6,309,799 - +1.4 6,309,799 - +0.2 6,309,817 + +1.7 6,309,817 + +0.7 6,309,846 + +0.1 6,309,846 + +2.0 6,309,846 + +0.9 6,309,846 + +0.1 6,309,846 + +1.3 6,309,847 - +0.7 6,309,847 - +1.6 6,309,847 - +1.5 6,309,847 - +1.3 6,309,847 - +1.1 6,310,050 + Pf6N2E2_5491 0.28 +0.8 6,310,050 + Pf6N2E2_5491 0.28 +1.1 6,310,050 + Pf6N2E2_5491 0.28 +1.9 6,310,050 + Pf6N2E2_5491 0.28 +2.2 6,310,050 + Pf6N2E2_5491 0.28 +0.3 6,310,050 + Pf6N2E2_5491 0.28 -2.0 6,310,050 + Pf6N2E2_5491 0.28 +1.5 6,310,051 - Pf6N2E2_5491 0.28 +1.2 6,310,051 - Pf6N2E2_5491 0.28 +2.0 6,310,051 - Pf6N2E2_5491 0.28 +1.9 6,310,054 + Pf6N2E2_5491 0.29 +0.5 6,310,054 + Pf6N2E2_5491 0.29 +2.0 6,310,055 - Pf6N2E2_5491 0.29 +0.8 6,310,055 - Pf6N2E2_5491 0.29 +1.0 6,310,055 - Pf6N2E2_5491 0.29 -0.9 6,310,139 + Pf6N2E2_5491 0.36 -0.0 6,310,140 - Pf6N2E2_5491 0.36 +1.9 6,310,142 + Pf6N2E2_5491 0.36 +0.6 6,310,142 + Pf6N2E2_5491 0.36 +2.1 6,310,142 + Pf6N2E2_5491 0.36 +2.8 6,310,143 - Pf6N2E2_5491 0.37 +1.0 6,310,143 - Pf6N2E2_5491 0.37 +2.3 6,310,158 + Pf6N2E2_5491 0.38 +1.0 6,310,159 - Pf6N2E2_5491 0.38 +0.9 6,310,249 - Pf6N2E2_5491 0.46 +2.2 6,310,249 - Pf6N2E2_5491 0.46 +0.6 6,310,249 - Pf6N2E2_5491 0.46 +0.8 6,310,258 + Pf6N2E2_5491 0.47 -0.0 6,310,258 + Pf6N2E2_5491 0.47 +0.1 6,310,258 + Pf6N2E2_5491 0.47 -0.2 6,310,259 - Pf6N2E2_5491 0.47 +1.4 6,310,259 - Pf6N2E2_5491 0.47 -0.6 6,310,259 - Pf6N2E2_5491 0.47 +4.2 6,310,567 + Pf6N2E2_5491 0.75 +1.0 6,310,567 + Pf6N2E2_5491 0.75 +0.7 6,310,567 + Pf6N2E2_5491 0.75 +0.8 6,310,567 + Pf6N2E2_5491 0.75 +1.9 6,310,568 - Pf6N2E2_5491 0.75 +1.1 6,310,568 - Pf6N2E2_5491 0.75 +0.3 6,310,568 - Pf6N2E2_5491 0.75 +1.5 6,310,568 - Pf6N2E2_5491 0.75 -1.2 6,310,578 + Pf6N2E2_5491 0.76 +0.6 6,310,888 - +1.4 6,310,930 + +0.9 6,310,930 + +0.6 6,311,389 + Pf6N2E2_5492 0.59 +0.7 6,311,389 + Pf6N2E2_5492 0.59 -0.8 6,311,389 + Pf6N2E2_5492 0.59 -0.8 6,311,390 - Pf6N2E2_5492 0.59 +1.8 6,311,390 - Pf6N2E2_5492 0.59 +0.9 6,311,509 - Pf6N2E2_5492 0.72 +0.8 6,311,666 + Pf6N2E2_5492 0.89 +1.3 6,311,666 + Pf6N2E2_5492 0.89 +0.9 6,311,666 + Pf6N2E2_5492 0.89 -0.8 6,311,666 + Pf6N2E2_5492 0.89 -1.5 6,311,667 - Pf6N2E2_5492 0.89 +0.9 6,311,713 + -0.5 6,311,713 + +0.1 6,311,714 - +0.4 6,311,714 - +1.4
Or see this region's nucleotide sequence