Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5417 and Pf6N2E2_5418 are separated by 261 nucleotides Pf6N2E2_5418 and Pf6N2E2_5419 are separated by 8 nucleotides Pf6N2E2_5419 and Pf6N2E2_5420 are separated by 60 nucleotides
Pf6N2E2_5417 - Valyl-tRNA synthetase (EC 6.1.1.9), at 6,220,443 to 6,223,289
_5417
Pf6N2E2_5418 - FIG00957284: hypothetical protein, at 6,223,551 to 6,223,916
_5418
Pf6N2E2_5419 - DNA polymerase III chi subunit (EC 2.7.7.7), at 6,223,925 to 6,224,353
_5419
Pf6N2E2_5420 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 6,224,414 to 6,225,904
_5420
Position (kb)
6223
6224
6225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6223.336 kb on - strand at 6223.336 kb on - strand at 6223.336 kb on - strand at 6223.382 kb on + strand at 6223.383 kb on - strand at 6223.386 kb on - strand at 6223.552 kb on + strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.558 kb on + strand at 6223.558 kb on + strand at 6223.559 kb on - strand at 6223.559 kb on - strand at 6223.919 kb on + strand at 6223.937 kb on + strand at 6223.938 kb on - strand at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.334 kb on + strand at 6224.334 kb on + strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.355 kb on - strand at 6224.439 kb on + strand at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.153 kb on + strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.204 kb on + strand, within Pf6N2E2_5420
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,223,336 - +0.7 6,223,336 - +3.0 6,223,336 - -0.3 6,223,382 + +1.8 6,223,383 - +0.7 6,223,386 - +0.1 6,223,552 + -3.0 6,223,553 - -1.5 6,223,553 - +0.4 6,223,553 - -0.8 6,223,558 + +1.4 6,223,558 + -0.9 6,223,559 - -0.9 6,223,559 - -3.0 6,223,919 + -0.9 6,223,937 + -2.3 6,223,938 - +0.8 6,224,247 + Pf6N2E2_5419 0.75 +0.2 6,224,247 + Pf6N2E2_5419 0.75 -1.2 6,224,248 - Pf6N2E2_5419 0.75 +0.6 6,224,248 - Pf6N2E2_5419 0.75 +0.8 6,224,248 - Pf6N2E2_5419 0.75 +0.3 6,224,248 - Pf6N2E2_5419 0.75 -3.0 6,224,334 + +0.8 6,224,334 + -0.4 6,224,335 - +0.3 6,224,335 - -0.5 6,224,335 - +0.0 6,224,335 - +0.4 6,224,335 - +0.8 6,224,355 - +0.6 6,224,439 + +0.2 6,224,641 - Pf6N2E2_5420 0.15 -1.3 6,224,641 - Pf6N2E2_5420 0.15 +0.4 6,224,824 - Pf6N2E2_5420 0.27 -0.0 6,224,824 - Pf6N2E2_5420 0.27 -0.7 6,224,824 - Pf6N2E2_5420 0.27 -1.1 6,225,043 - Pf6N2E2_5420 0.42 +0.3 6,225,043 - Pf6N2E2_5420 0.42 -0.3 6,225,043 - Pf6N2E2_5420 0.42 +0.4 6,225,043 - Pf6N2E2_5420 0.42 +0.9 6,225,153 + Pf6N2E2_5420 0.50 +0.1 6,225,154 - Pf6N2E2_5420 0.50 -2.7 6,225,154 - Pf6N2E2_5420 0.50 -4.0 6,225,154 - Pf6N2E2_5420 0.50 +0.3 6,225,204 + Pf6N2E2_5420 0.53 +0.5
Or see this region's nucleotide sequence