Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5400 and Pf6N2E2_5401 are separated by 99 nucleotides Pf6N2E2_5401 and Pf6N2E2_5402 are separated by 473 nucleotides
Pf6N2E2_5400 - ATP-dependent RNA helicase RhlB, at 6,204,799 to 6,206,274
_5400
Pf6N2E2_5401 - phospholipase/carboxylesterase family protein, at 6,206,374 to 6,207,030
_5401
Pf6N2E2_5402 - ABC transporter for D-Alanine, periplasmic substrate-binding component (from data) , at 6,207,504 to 6,208,535
_5402
Position (kb)
6206
6207
6208 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6205.667 kb on + strand, within Pf6N2E2_5400 at 6205.667 kb on + strand, within Pf6N2E2_5400 at 6205.705 kb on + strand, within Pf6N2E2_5400 at 6205.754 kb on + strand, within Pf6N2E2_5400 at 6205.754 kb on + strand, within Pf6N2E2_5400 at 6205.754 kb on + strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.755 kb on - strand, within Pf6N2E2_5400 at 6205.871 kb on + strand, within Pf6N2E2_5400 at 6205.990 kb on + strand, within Pf6N2E2_5400 at 6205.990 kb on + strand, within Pf6N2E2_5400 at 6205.991 kb on - strand, within Pf6N2E2_5400 at 6205.991 kb on - strand, within Pf6N2E2_5400 at 6206.297 kb on + strand at 6206.298 kb on - strand at 6206.312 kb on - strand at 6206.312 kb on - strand at 6206.425 kb on + strand at 6206.448 kb on - strand, within Pf6N2E2_5401 at 6206.482 kb on + strand, within Pf6N2E2_5401 at 6206.482 kb on + strand, within Pf6N2E2_5401 at 6206.482 kb on + strand, within Pf6N2E2_5401 at 6206.482 kb on + strand, within Pf6N2E2_5401 at 6206.483 kb on - strand, within Pf6N2E2_5401 at 6206.483 kb on - strand, within Pf6N2E2_5401 at 6206.483 kb on - strand, within Pf6N2E2_5401 at 6206.483 kb on - strand, within Pf6N2E2_5401 at 6206.486 kb on + strand, within Pf6N2E2_5401 at 6206.487 kb on - strand, within Pf6N2E2_5401 at 6206.487 kb on - strand, within Pf6N2E2_5401 at 6206.487 kb on - strand, within Pf6N2E2_5401 at 6206.487 kb on - strand, within Pf6N2E2_5401 at 6206.531 kb on + strand, within Pf6N2E2_5401 at 6206.531 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.609 kb on + strand, within Pf6N2E2_5401 at 6206.610 kb on - strand, within Pf6N2E2_5401 at 6206.610 kb on - strand, within Pf6N2E2_5401 at 6206.610 kb on - strand, within Pf6N2E2_5401 at 6206.610 kb on - strand, within Pf6N2E2_5401 at 6206.613 kb on + strand, within Pf6N2E2_5401 at 6206.613 kb on + strand, within Pf6N2E2_5401 at 6206.613 kb on + strand, within Pf6N2E2_5401 at 6206.613 kb on + strand, within Pf6N2E2_5401 at 6206.614 kb on - strand, within Pf6N2E2_5401 at 6206.614 kb on - strand, within Pf6N2E2_5401 at 6206.614 kb on - strand, within Pf6N2E2_5401 at 6206.669 kb on + strand, within Pf6N2E2_5401 at 6206.669 kb on + strand, within Pf6N2E2_5401 at 6206.670 kb on - strand, within Pf6N2E2_5401 at 6206.670 kb on - strand, within Pf6N2E2_5401 at 6206.722 kb on + strand, within Pf6N2E2_5401 at 6206.828 kb on + strand, within Pf6N2E2_5401 at 6206.980 kb on - strand at 6207.076 kb on + strand at 6207.076 kb on + strand at 6207.076 kb on + strand at 6207.077 kb on - strand at 6207.101 kb on + strand at 6207.105 kb on + strand at 6207.105 kb on + strand at 6207.106 kb on - strand at 6207.106 kb on - strand at 6207.145 kb on + strand at 6207.192 kb on - strand at 6207.209 kb on + strand at 6207.210 kb on - strand at 6207.227 kb on + strand at 6207.227 kb on + strand at 6207.227 kb on + strand at 6207.227 kb on + strand at 6207.227 kb on + strand at 6207.228 kb on - strand at 6207.290 kb on + strand at 6207.315 kb on + strand at 6207.316 kb on - strand at 6207.376 kb on + strand at 6207.379 kb on + strand at 6207.380 kb on - strand at 6207.380 kb on - strand at 6207.380 kb on - strand at 6207.380 kb on - strand at 6207.388 kb on - strand at 6207.388 kb on - strand at 6207.511 kb on + strand at 6207.512 kb on - strand at 6207.512 kb on - strand at 6207.512 kb on - strand at 6207.722 kb on - strand, within Pf6N2E2_5402 at 6207.879 kb on + strand, within Pf6N2E2_5402 at 6207.879 kb on + strand, within Pf6N2E2_5402 at 6207.880 kb on - strand, within Pf6N2E2_5402 at 6207.880 kb on - strand, within Pf6N2E2_5402 at 6207.882 kb on + strand, within Pf6N2E2_5402 at 6207.882 kb on + strand, within Pf6N2E2_5402 at 6207.882 kb on + strand, within Pf6N2E2_5402 at 6207.883 kb on - strand, within Pf6N2E2_5402 at 6207.883 kb on - strand, within Pf6N2E2_5402 at 6207.883 kb on - strand, within Pf6N2E2_5402 at 6207.999 kb on + strand, within Pf6N2E2_5402 at 6208.000 kb on - strand, within Pf6N2E2_5402 at 6208.000 kb on - strand, within Pf6N2E2_5402 at 6208.024 kb on - strand, within Pf6N2E2_5402
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,205,667 + Pf6N2E2_5400 0.59 +0.7 6,205,667 + Pf6N2E2_5400 0.59 -0.5 6,205,705 + Pf6N2E2_5400 0.61 +0.2 6,205,754 + Pf6N2E2_5400 0.65 +0.0 6,205,754 + Pf6N2E2_5400 0.65 +0.7 6,205,754 + Pf6N2E2_5400 0.65 +0.7 6,205,755 - Pf6N2E2_5400 0.65 +0.4 6,205,755 - Pf6N2E2_5400 0.65 +0.9 6,205,755 - Pf6N2E2_5400 0.65 +0.4 6,205,755 - Pf6N2E2_5400 0.65 +0.6 6,205,755 - Pf6N2E2_5400 0.65 +0.2 6,205,755 - Pf6N2E2_5400 0.65 -0.6 6,205,871 + Pf6N2E2_5400 0.73 +1.5 6,205,990 + Pf6N2E2_5400 0.81 +1.0 6,205,990 + Pf6N2E2_5400 0.81 +1.7 6,205,991 - Pf6N2E2_5400 0.81 +0.9 6,205,991 - Pf6N2E2_5400 0.81 -0.5 6,206,297 + +0.4 6,206,298 - -1.1 6,206,312 - -1.3 6,206,312 - -0.8 6,206,425 + +2.1 6,206,448 - Pf6N2E2_5401 0.11 -0.3 6,206,482 + Pf6N2E2_5401 0.16 +0.2 6,206,482 + Pf6N2E2_5401 0.16 +1.0 6,206,482 + Pf6N2E2_5401 0.16 -0.5 6,206,482 + Pf6N2E2_5401 0.16 +1.3 6,206,483 - Pf6N2E2_5401 0.17 -0.3 6,206,483 - Pf6N2E2_5401 0.17 -2.5 6,206,483 - Pf6N2E2_5401 0.17 -3.0 6,206,483 - Pf6N2E2_5401 0.17 -1.2 6,206,486 + Pf6N2E2_5401 0.17 +0.8 6,206,487 - Pf6N2E2_5401 0.17 -0.3 6,206,487 - Pf6N2E2_5401 0.17 -0.6 6,206,487 - Pf6N2E2_5401 0.17 +0.6 6,206,487 - Pf6N2E2_5401 0.17 -1.9 6,206,531 + Pf6N2E2_5401 0.24 +0.7 6,206,531 + Pf6N2E2_5401 0.24 +1.7 6,206,609 + Pf6N2E2_5401 0.36 +0.6 6,206,609 + Pf6N2E2_5401 0.36 +1.6 6,206,609 + Pf6N2E2_5401 0.36 +1.1 6,206,609 + Pf6N2E2_5401 0.36 +1.0 6,206,609 + Pf6N2E2_5401 0.36 +0.7 6,206,609 + Pf6N2E2_5401 0.36 +0.6 6,206,609 + Pf6N2E2_5401 0.36 +2.2 6,206,610 - Pf6N2E2_5401 0.36 +0.4 6,206,610 - Pf6N2E2_5401 0.36 -0.6 6,206,610 - Pf6N2E2_5401 0.36 -1.0 6,206,610 - Pf6N2E2_5401 0.36 -1.6 6,206,613 + Pf6N2E2_5401 0.36 +1.1 6,206,613 + Pf6N2E2_5401 0.36 +0.8 6,206,613 + Pf6N2E2_5401 0.36 +1.1 6,206,613 + Pf6N2E2_5401 0.36 +1.5 6,206,614 - Pf6N2E2_5401 0.37 -0.6 6,206,614 - Pf6N2E2_5401 0.37 +1.1 6,206,614 - Pf6N2E2_5401 0.37 -0.5 6,206,669 + Pf6N2E2_5401 0.45 +0.4 6,206,669 + Pf6N2E2_5401 0.45 +0.2 6,206,670 - Pf6N2E2_5401 0.45 -1.2 6,206,670 - Pf6N2E2_5401 0.45 -0.5 6,206,722 + Pf6N2E2_5401 0.53 +1.0 6,206,828 + Pf6N2E2_5401 0.69 +1.3 6,206,980 - -0.5 6,207,076 + +0.8 6,207,076 + +0.5 6,207,076 + +2.0 6,207,077 - +0.4 6,207,101 + +0.8 6,207,105 + +1.0 6,207,105 + +1.4 6,207,106 - -3.5 6,207,106 - -2.1 6,207,145 + +1.6 6,207,192 - -1.8 6,207,209 + +2.6 6,207,210 - -3.7 6,207,227 + +1.6 6,207,227 + +2.7 6,207,227 + +1.9 6,207,227 + +1.4 6,207,227 + +1.6 6,207,228 - +1.1 6,207,290 + -0.3 6,207,315 + +2.3 6,207,316 - -3.2 6,207,376 + +1.8 6,207,379 + +1.8 6,207,380 - -1.9 6,207,380 - -3.5 6,207,380 - -0.7 6,207,380 - -3.3 6,207,388 - -1.8 6,207,388 - -2.0 6,207,511 + -2.9 6,207,512 - -1.5 6,207,512 - -0.9 6,207,512 - -2.7 6,207,722 - Pf6N2E2_5402 0.21 -0.9 6,207,879 + Pf6N2E2_5402 0.36 -2.5 6,207,879 + Pf6N2E2_5402 0.36 -4.1 6,207,880 - Pf6N2E2_5402 0.36 -0.4 6,207,880 - Pf6N2E2_5402 0.36 -2.5 6,207,882 + Pf6N2E2_5402 0.37 +0.9 6,207,882 + Pf6N2E2_5402 0.37 -0.2 6,207,882 + Pf6N2E2_5402 0.37 -0.3 6,207,883 - Pf6N2E2_5402 0.37 -2.5 6,207,883 - Pf6N2E2_5402 0.37 -1.8 6,207,883 - Pf6N2E2_5402 0.37 -1.5 6,207,999 + Pf6N2E2_5402 0.48 -1.2 6,208,000 - Pf6N2E2_5402 0.48 -0.3 6,208,000 - Pf6N2E2_5402 0.48 -3.6 6,208,024 - Pf6N2E2_5402 0.50 -0.9
Or see this region's nucleotide sequence