Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_528 and Pf6N2E2_529 are separated by 792 nucleotides Pf6N2E2_529 and Pf6N2E2_530 are separated by 171 nucleotides
Pf6N2E2_528 - FIG00961088: hypothetical protein, at 597,724 to 597,954
_528
Pf6N2E2_529 - FIG00954439: hypothetical protein, at 598,747 to 599,196
_529
Pf6N2E2_530 - Histidine transporter, periplasmic histidine-binding protein, at 599,368 to 600,378
_530
Position (kb) 598
599
600 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 598.058 kb on + strand at 598.058 kb on + strand at 598.058 kb on + strand at 598.058 kb on + strand at 598.059 kb on - strand at 598.059 kb on - strand at 598.059 kb on - strand at 598.059 kb on - strand at 598.059 kb on - strand at 598.059 kb on - strand at 598.077 kb on + strand at 598.077 kb on + strand at 598.077 kb on + strand at 598.128 kb on + strand at 598.150 kb on + strand at 598.151 kb on - strand at 598.335 kb on + strand at 598.342 kb on + strand at 598.369 kb on + strand at 598.393 kb on + strand at 598.425 kb on + strand at 598.426 kb on - strand at 598.429 kb on + strand at 598.429 kb on + strand at 598.429 kb on + strand at 598.430 kb on - strand at 598.430 kb on - strand at 598.430 kb on - strand at 598.495 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.582 kb on + strand at 598.583 kb on - strand at 598.583 kb on - strand at 598.583 kb on - strand at 598.583 kb on - strand at 598.583 kb on - strand at 598.583 kb on - strand at 598.584 kb on + strand at 598.584 kb on + strand at 598.584 kb on + strand at 598.584 kb on + strand at 598.585 kb on - strand at 598.646 kb on + strand at 598.655 kb on - strand at 598.734 kb on - strand at 598.734 kb on - strand at 598.734 kb on - strand at 598.737 kb on + strand at 598.737 kb on + strand at 598.737 kb on + strand at 598.757 kb on + strand at 598.764 kb on - strand at 598.764 kb on - strand at 598.816 kb on + strand, within Pf6N2E2_529 at 598.816 kb on + strand, within Pf6N2E2_529 at 598.817 kb on - strand, within Pf6N2E2_529 at 598.842 kb on + strand, within Pf6N2E2_529 at 598.843 kb on - strand, within Pf6N2E2_529 at 598.843 kb on - strand, within Pf6N2E2_529 at 598.846 kb on + strand, within Pf6N2E2_529 at 598.846 kb on + strand, within Pf6N2E2_529 at 598.846 kb on + strand, within Pf6N2E2_529 at 598.846 kb on + strand, within Pf6N2E2_529 at 598.847 kb on - strand, within Pf6N2E2_529 at 598.847 kb on - strand, within Pf6N2E2_529 at 598.847 kb on - strand, within Pf6N2E2_529 at 598.847 kb on - strand, within Pf6N2E2_529 at 598.847 kb on - strand, within Pf6N2E2_529 at 598.999 kb on + strand, within Pf6N2E2_529 at 599.014 kb on + strand, within Pf6N2E2_529 at 599.298 kb on + strand at 599.298 kb on + strand at 599.354 kb on - strand at 599.569 kb on - strand, within Pf6N2E2_530 at 599.725 kb on + strand, within Pf6N2E2_530 at 599.726 kb on - strand, within Pf6N2E2_530 at 599.803 kb on + strand, within Pf6N2E2_530 at 599.926 kb on + strand, within Pf6N2E2_530 at 599.926 kb on + strand, within Pf6N2E2_530 at 599.926 kb on + strand, within Pf6N2E2_530 at 599.926 kb on + strand, within Pf6N2E2_530 at 599.926 kb on + strand, within Pf6N2E2_530 at 599.927 kb on - strand, within Pf6N2E2_530 at 599.927 kb on - strand, within Pf6N2E2_530 at 599.927 kb on - strand, within Pf6N2E2_530 at 600.010 kb on + strand, within Pf6N2E2_530 at 600.010 kb on + strand, within Pf6N2E2_530 at 600.010 kb on + strand, within Pf6N2E2_530 at 600.011 kb on - strand, within Pf6N2E2_530 at 600.013 kb on + strand, within Pf6N2E2_530 at 600.013 kb on + strand, within Pf6N2E2_530 at 600.014 kb on - strand, within Pf6N2E2_530
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 598,058 + +0.0 598,058 + +1.4 598,058 + +1.3 598,058 + +0.0 598,059 - -0.8 598,059 - -0.6 598,059 - +0.4 598,059 - +0.1 598,059 - -1.3 598,059 - +0.4 598,077 + -0.3 598,077 + +2.4 598,077 + +1.6 598,128 + +1.7 598,150 + +0.7 598,151 - -0.9 598,335 + +1.6 598,342 + +1.7 598,369 + +0.1 598,393 + +1.6 598,425 + +1.4 598,426 - -0.3 598,429 + +0.7 598,429 + +2.8 598,429 + +0.1 598,430 - -1.8 598,430 - +1.7 598,430 - -2.7 598,495 + +1.0 598,582 + +0.1 598,582 + +1.5 598,582 + +0.7 598,582 + +0.1 598,582 + -0.3 598,582 + +0.3 598,582 + -0.8 598,582 + +1.5 598,582 + +0.3 598,582 + +0.5 598,582 + +0.8 598,582 + +0.9 598,582 + -0.5 598,583 - -0.9 598,583 - +0.6 598,583 - -2.4 598,583 - +0.2 598,583 - -0.8 598,583 - -0.8 598,584 + +1.1 598,584 + +1.3 598,584 + +3.7 598,584 + +2.3 598,585 - -0.8 598,646 + -0.3 598,655 - +0.9 598,734 - -0.3 598,734 - -0.8 598,734 - -0.7 598,737 + -1.3 598,737 + -1.7 598,737 + +0.1 598,757 + -1.3 598,764 - -0.5 598,764 - -1.3 598,816 + Pf6N2E2_529 0.15 -1.5 598,816 + Pf6N2E2_529 0.15 +0.3 598,817 - Pf6N2E2_529 0.16 -1.3 598,842 + Pf6N2E2_529 0.21 -0.8 598,843 - Pf6N2E2_529 0.21 +0.1 598,843 - Pf6N2E2_529 0.21 +1.1 598,846 + Pf6N2E2_529 0.22 -2.1 598,846 + Pf6N2E2_529 0.22 +0.3 598,846 + Pf6N2E2_529 0.22 -2.0 598,846 + Pf6N2E2_529 0.22 -1.7 598,847 - Pf6N2E2_529 0.22 -2.1 598,847 - Pf6N2E2_529 0.22 -1.6 598,847 - Pf6N2E2_529 0.22 -2.8 598,847 - Pf6N2E2_529 0.22 -0.8 598,847 - Pf6N2E2_529 0.22 +1.1 598,999 + Pf6N2E2_529 0.56 -1.8 599,014 + Pf6N2E2_529 0.59 -0.3 599,298 + -1.6 599,298 + -1.3 599,354 - -1.2 599,569 - Pf6N2E2_530 0.20 +0.4 599,725 + Pf6N2E2_530 0.35 -2.4 599,726 - Pf6N2E2_530 0.35 -0.8 599,803 + Pf6N2E2_530 0.43 +0.2 599,926 + Pf6N2E2_530 0.55 +0.1 599,926 + Pf6N2E2_530 0.55 -1.3 599,926 + Pf6N2E2_530 0.55 -0.5 599,926 + Pf6N2E2_530 0.55 -2.3 599,926 + Pf6N2E2_530 0.55 +2.2 599,927 - Pf6N2E2_530 0.55 -2.3 599,927 - Pf6N2E2_530 0.55 -0.7 599,927 - Pf6N2E2_530 0.55 -0.3 600,010 + Pf6N2E2_530 0.64 +0.3 600,010 + Pf6N2E2_530 0.64 -2.0 600,010 + Pf6N2E2_530 0.64 -0.5 600,011 - Pf6N2E2_530 0.64 -1.1 600,013 + Pf6N2E2_530 0.64 +0.5 600,013 + Pf6N2E2_530 0.64 -0.2 600,014 - Pf6N2E2_530 0.64 +1.3
Or see this region's nucleotide sequence