Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5216

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5214 and Pf6N2E2_5215 are separated by 166 nucleotidesPf6N2E2_5215 and Pf6N2E2_5216 are separated by 143 nucleotidesPf6N2E2_5216 and Pf6N2E2_6156 are separated by 50 nucleotidesPf6N2E2_6156 and Pf6N2E2_5217 are separated by 37 nucleotides Pf6N2E2_5214 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 6,012,663 to 6,013,247 _5214 Pf6N2E2_5215 - LSU ribosomal protein L25p, at 6,013,414 to 6,014,016 _5215 Pf6N2E2_5216 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 6,014,160 to 6,015,101 _5216 Pf6N2E2_6156 - tRNA-Gln-TTG, at 6,015,152 to 6,015,223 _6156 Pf6N2E2_5217 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 6,015,261 to 6,016,112 _5217 Position (kb) 6014 6015 6016Strain fitness (log2 ratio) -1 0 1at 6015.126 kb on - strandat 6015.128 kb on - strandat 6015.128 kb on - strandat 6015.128 kb on - strandat 6015.142 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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6,015,126 - +0.2
6,015,128 - -1.3
6,015,128 - -0.5
6,015,128 - +0.7
6,015,142 - -0.6

Or see this region's nucleotide sequence