Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4820

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4819 and Pf6N2E2_4820 are separated by 78 nucleotidesPf6N2E2_4820 and Pf6N2E2_4821 overlap by 4 nucleotidesPf6N2E2_4821 and Pf6N2E2_4822 are separated by 70 nucleotides Pf6N2E2_4819 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,572,498 to 5,573,646 _4819 Pf6N2E2_4820 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 5,573,725 to 5,574,399 _4820 Pf6N2E2_4821 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 5,574,396 to 5,575,214 _4821 Pf6N2E2_4822 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 5,575,285 to 5,576,766 _4822 Position (kb) 5573 5574 5575Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 5572.819 kb on + strand, within Pf6N2E2_4819at 5572.819 kb on + strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.820 kb on - strand, within Pf6N2E2_4819at 5572.848 kb on + strand, within Pf6N2E2_4819at 5572.848 kb on + strand, within Pf6N2E2_4819at 5572.848 kb on + strand, within Pf6N2E2_4819at 5572.849 kb on - strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.990 kb on + strand, within Pf6N2E2_4819at 5572.991 kb on - strand, within Pf6N2E2_4819at 5572.991 kb on - strand, within Pf6N2E2_4819at 5572.991 kb on - strand, within Pf6N2E2_4819at 5572.991 kb on - strand, within Pf6N2E2_4819at 5573.098 kb on + strand, within Pf6N2E2_4819at 5573.098 kb on + strand, within Pf6N2E2_4819at 5573.099 kb on - strand, within Pf6N2E2_4819at 5573.182 kb on + strand, within Pf6N2E2_4819at 5573.182 kb on + strand, within Pf6N2E2_4819at 5573.182 kb on + strand, within Pf6N2E2_4819at 5573.183 kb on - strand, within Pf6N2E2_4819at 5573.183 kb on - strand, within Pf6N2E2_4819at 5573.183 kb on - strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.337 kb on + strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.338 kb on - strand, within Pf6N2E2_4819at 5573.350 kb on + strand, within Pf6N2E2_4819at 5573.350 kb on + strand, within Pf6N2E2_4819at 5573.351 kb on - strand, within Pf6N2E2_4819at 5573.351 kb on - strand, within Pf6N2E2_4819at 5573.475 kb on + strand, within Pf6N2E2_4819at 5573.475 kb on + strand, within Pf6N2E2_4819at 5573.476 kb on - strand, within Pf6N2E2_4819at 5573.476 kb on - strand, within Pf6N2E2_4819at 5573.476 kb on - strand, within Pf6N2E2_4819at 5573.476 kb on - strand, within Pf6N2E2_4819at 5573.476 kb on - strand, within Pf6N2E2_4819at 5573.499 kb on - strand, within Pf6N2E2_4819at 5573.570 kb on + strandat 5573.630 kb on - strandat 5573.641 kb on + strandat 5573.642 kb on - strandat 5573.681 kb on + strandat 5573.681 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.683 kb on + strandat 5573.684 kb on - strandat 5573.684 kb on - strandat 5573.684 kb on - strandat 5573.684 kb on - strandat 5573.684 kb on - strandat 5574.550 kb on + strand, within Pf6N2E2_4821at 5574.764 kb on + strand, within Pf6N2E2_4821at 5574.764 kb on + strand, within Pf6N2E2_4821at 5574.765 kb on - strand, within Pf6N2E2_4821at 5574.765 kb on - strand, within Pf6N2E2_4821at 5574.848 kb on + strand, within Pf6N2E2_4821at 5574.999 kb on + strand, within Pf6N2E2_4821at 5575.006 kb on - strand, within Pf6N2E2_4821at 5575.069 kb on + strand, within Pf6N2E2_4821at 5575.069 kb on + strand, within Pf6N2E2_4821at 5575.069 kb on + strand, within Pf6N2E2_4821at 5575.070 kb on - strand, within Pf6N2E2_4821at 5575.102 kb on + strand, within Pf6N2E2_4821at 5575.102 kb on + strand, within Pf6N2E2_4821at 5575.102 kb on + strand, within Pf6N2E2_4821at 5575.103 kb on - strand, within Pf6N2E2_4821at 5575.103 kb on - strand, within Pf6N2E2_4821at 5575.103 kb on - strand, within Pf6N2E2_4821at 5575.103 kb on - strand, within Pf6N2E2_4821at 5575.103 kb on - strand, within Pf6N2E2_4821at 5575.248 kb on + strandat 5575.248 kb on + strandat 5575.249 kb on - strandat 5575.249 kb on - strandat 5575.324 kb on + strandat 5575.325 kb on - strandat 5575.385 kb on - strandat 5575.385 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,572,819 + Pf6N2E2_4819 0.28 -1.8
5,572,819 + Pf6N2E2_4819 0.28 -1.2
5,572,820 - Pf6N2E2_4819 0.28 +0.6
5,572,820 - Pf6N2E2_4819 0.28 +0.2
5,572,820 - Pf6N2E2_4819 0.28 +0.9
5,572,820 - Pf6N2E2_4819 0.28 -0.0
5,572,820 - Pf6N2E2_4819 0.28 +0.6
5,572,820 - Pf6N2E2_4819 0.28 +0.4
5,572,848 + Pf6N2E2_4819 0.30 -0.5
5,572,848 + Pf6N2E2_4819 0.30 -1.6
5,572,848 + Pf6N2E2_4819 0.30 -1.2
5,572,849 - Pf6N2E2_4819 0.31 -0.9
5,572,990 + Pf6N2E2_4819 0.43 +1.7
5,572,990 + Pf6N2E2_4819 0.43 +0.0
5,572,990 + Pf6N2E2_4819 0.43 +0.1
5,572,990 + Pf6N2E2_4819 0.43 +0.0
5,572,990 + Pf6N2E2_4819 0.43 +0.4
5,572,990 + Pf6N2E2_4819 0.43 +1.0
5,572,991 - Pf6N2E2_4819 0.43 +1.0
5,572,991 - Pf6N2E2_4819 0.43 +0.6
5,572,991 - Pf6N2E2_4819 0.43 -0.5
5,572,991 - Pf6N2E2_4819 0.43 +0.0
5,573,098 + Pf6N2E2_4819 0.52 -1.8
5,573,098 + Pf6N2E2_4819 0.52 +1.8
5,573,099 - Pf6N2E2_4819 0.52 +1.1
5,573,182 + Pf6N2E2_4819 0.60 +0.7
5,573,182 + Pf6N2E2_4819 0.60 +0.4
5,573,182 + Pf6N2E2_4819 0.60 -0.1
5,573,183 - Pf6N2E2_4819 0.60 -0.0
5,573,183 - Pf6N2E2_4819 0.60 +0.1
5,573,183 - Pf6N2E2_4819 0.60 +0.6
5,573,337 + Pf6N2E2_4819 0.73 +0.6
5,573,337 + Pf6N2E2_4819 0.73 -0.0
5,573,337 + Pf6N2E2_4819 0.73 +0.8
5,573,337 + Pf6N2E2_4819 0.73 -0.2
5,573,337 + Pf6N2E2_4819 0.73 +0.4
5,573,337 + Pf6N2E2_4819 0.73 +0.2
5,573,337 + Pf6N2E2_4819 0.73 +1.3
5,573,337 + Pf6N2E2_4819 0.73 +1.2
5,573,337 + Pf6N2E2_4819 0.73 -0.2
5,573,338 - Pf6N2E2_4819 0.73 +0.2
5,573,338 - Pf6N2E2_4819 0.73 -0.3
5,573,338 - Pf6N2E2_4819 0.73 -1.4
5,573,338 - Pf6N2E2_4819 0.73 -0.0
5,573,338 - Pf6N2E2_4819 0.73 -1.2
5,573,338 - Pf6N2E2_4819 0.73 +0.9
5,573,338 - Pf6N2E2_4819 0.73 -0.5
5,573,338 - Pf6N2E2_4819 0.73 +0.4
5,573,338 - Pf6N2E2_4819 0.73 +0.4
5,573,350 + Pf6N2E2_4819 0.74 -1.4
5,573,350 + Pf6N2E2_4819 0.74 +0.2
5,573,351 - Pf6N2E2_4819 0.74 +5.4
5,573,351 - Pf6N2E2_4819 0.74 -0.1
5,573,475 + Pf6N2E2_4819 0.85 +0.3
5,573,475 + Pf6N2E2_4819 0.85 +0.6
5,573,476 - Pf6N2E2_4819 0.85 -1.2
5,573,476 - Pf6N2E2_4819 0.85 -0.0
5,573,476 - Pf6N2E2_4819 0.85 +0.5
5,573,476 - Pf6N2E2_4819 0.85 +0.7
5,573,476 - Pf6N2E2_4819 0.85 -1.1
5,573,499 - Pf6N2E2_4819 0.87 -0.9
5,573,570 + -0.7
5,573,630 - +0.5
5,573,641 + +0.6
5,573,642 - +1.1
5,573,681 + -0.0
5,573,681 + +0.0
5,573,683 + -0.4
5,573,683 + +1.2
5,573,683 + -1.1
5,573,683 + -0.7
5,573,683 + +0.2
5,573,683 + +1.0
5,573,683 + -0.0
5,573,683 + +2.0
5,573,683 + +0.1
5,573,684 - +2.3
5,573,684 - +3.1
5,573,684 - +1.5
5,573,684 - +1.5
5,573,684 - +1.0
5,574,550 + Pf6N2E2_4821 0.19 -1.6
5,574,764 + Pf6N2E2_4821 0.45 +0.6
5,574,764 + Pf6N2E2_4821 0.45 -0.5
5,574,765 - Pf6N2E2_4821 0.45 -1.7
5,574,765 - Pf6N2E2_4821 0.45 -0.8
5,574,848 + Pf6N2E2_4821 0.55 -0.4
5,574,999 + Pf6N2E2_4821 0.74 -1.2
5,575,006 - Pf6N2E2_4821 0.74 -0.8
5,575,069 + Pf6N2E2_4821 0.82 -0.1
5,575,069 + Pf6N2E2_4821 0.82 +0.1
5,575,069 + Pf6N2E2_4821 0.82 -2.3
5,575,070 - Pf6N2E2_4821 0.82 -1.7
5,575,102 + Pf6N2E2_4821 0.86 -1.6
5,575,102 + Pf6N2E2_4821 0.86 +0.7
5,575,102 + Pf6N2E2_4821 0.86 -0.6
5,575,103 - Pf6N2E2_4821 0.86 +0.2
5,575,103 - Pf6N2E2_4821 0.86 -1.6
5,575,103 - Pf6N2E2_4821 0.86 -2.2
5,575,103 - Pf6N2E2_4821 0.86 -1.0
5,575,103 - Pf6N2E2_4821 0.86 -0.8
5,575,248 + +0.5
5,575,248 + -0.6
5,575,249 - -0.4
5,575,249 - +0.8
5,575,324 + +0.8
5,575,325 - +0.5
5,575,385 - +0.2
5,575,385 - +0.3

Or see this region's nucleotide sequence