Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4653 and Pf6N2E2_4654 are separated by 31 nucleotides Pf6N2E2_4654 and Pf6N2E2_4655 are separated by 39 nucleotides
Pf6N2E2_4653 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 5,383,136 to 5,384,131
_4653
Pf6N2E2_4654 - hypothetical protein, at 5,384,163 to 5,384,384
_4654
Pf6N2E2_4655 - Transketolase (EC 2.2.1.1), at 5,384,424 to 5,386,421
_4655
Position (kb)
5384
5385 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 5383.203 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.493 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.124 kb on - strand at 5384.124 kb on - strand at 5384.132 kb on - strand at 5384.152 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.222 kb on - strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,383,203 - +0.6 5,383,209 - +1.6 5,383,209 - +0.6 5,383,209 - +0.5 5,383,213 - +2.3 5,383,213 - -0.8 5,383,213 - +2.4 5,383,213 - +0.4 5,383,213 - +0.6 5,383,213 - +0.8 5,383,332 - Pf6N2E2_4653 0.20 +0.9 5,383,332 - Pf6N2E2_4653 0.20 +1.2 5,383,332 - Pf6N2E2_4653 0.20 +0.6 5,383,332 - Pf6N2E2_4653 0.20 +0.3 5,383,332 - Pf6N2E2_4653 0.20 +1.3 5,383,332 - Pf6N2E2_4653 0.20 +0.8 5,383,431 - Pf6N2E2_4653 0.30 +1.0 5,383,431 - Pf6N2E2_4653 0.30 +1.0 5,383,493 - Pf6N2E2_4653 0.36 +1.4 5,383,650 - Pf6N2E2_4653 0.52 +0.9 5,383,650 - Pf6N2E2_4653 0.52 +1.3 5,383,650 - Pf6N2E2_4653 0.52 +0.7 5,383,650 - Pf6N2E2_4653 0.52 -0.0 5,383,650 - Pf6N2E2_4653 0.52 -0.4 5,383,779 - Pf6N2E2_4653 0.65 +0.7 5,383,779 - Pf6N2E2_4653 0.65 +0.9 5,383,779 - Pf6N2E2_4653 0.65 +1.2 5,384,040 - +3.8 5,384,040 - +3.7 5,384,040 - +3.9 5,384,124 - +4.0 5,384,124 - +4.9 5,384,132 - +2.4 5,384,152 - +2.1 5,384,165 - +1.3 5,384,165 - +2.0 5,384,165 - +2.6 5,384,222 - Pf6N2E2_4654 0.27 +3.5 5,384,252 + Pf6N2E2_4654 0.40 +1.7 5,384,252 + Pf6N2E2_4654 0.40 +0.3 5,384,253 - Pf6N2E2_4654 0.41 +2.6 5,384,253 - Pf6N2E2_4654 0.41 +4.7 5,384,253 - Pf6N2E2_4654 0.41 +4.7 5,384,253 - Pf6N2E2_4654 0.41 +3.4 5,384,253 - Pf6N2E2_4654 0.41 +3.9 5,384,253 - Pf6N2E2_4654 0.41 +3.5 5,384,253 - Pf6N2E2_4654 0.41 +4.9 5,384,253 - Pf6N2E2_4654 0.41 +4.0 5,384,295 + Pf6N2E2_4654 0.59 +0.4 5,384,295 + Pf6N2E2_4654 0.59 -0.9 5,384,295 + Pf6N2E2_4654 0.59 +0.0 5,384,295 + Pf6N2E2_4654 0.59 -1.0 5,384,296 - Pf6N2E2_4654 0.60 +5.4 5,384,296 - Pf6N2E2_4654 0.60 +5.1 5,384,296 - Pf6N2E2_4654 0.60 +6.0 5,384,324 + Pf6N2E2_4654 0.73 -0.2 5,384,324 + Pf6N2E2_4654 0.73 -0.3 5,384,324 + Pf6N2E2_4654 0.73 -0.2 5,384,325 - Pf6N2E2_4654 0.73 +5.1 5,384,325 - Pf6N2E2_4654 0.73 +1.9 5,384,387 + +1.5
Or see this region's nucleotide sequence