Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4654

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4653 and Pf6N2E2_4654 are separated by 31 nucleotidesPf6N2E2_4654 and Pf6N2E2_4655 are separated by 39 nucleotides Pf6N2E2_4653 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 5,383,136 to 5,384,131 _4653 Pf6N2E2_4654 - hypothetical protein, at 5,384,163 to 5,384,384 _4654 Pf6N2E2_4655 - Transketolase (EC 2.2.1.1), at 5,384,424 to 5,386,421 _4655 Position (kb) 5384 5385Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 5383.203 kb on - strandat 5383.209 kb on - strandat 5383.209 kb on - strandat 5383.209 kb on - strandat 5383.213 kb on - strandat 5383.213 kb on - strandat 5383.213 kb on - strandat 5383.213 kb on - strandat 5383.213 kb on - strandat 5383.213 kb on - strandat 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.332 kb on - strand, within Pf6N2E2_4653at 5383.431 kb on - strand, within Pf6N2E2_4653at 5383.431 kb on - strand, within Pf6N2E2_4653at 5383.493 kb on - strand, within Pf6N2E2_4653at 5383.650 kb on - strand, within Pf6N2E2_4653at 5383.650 kb on - strand, within Pf6N2E2_4653at 5383.650 kb on - strand, within Pf6N2E2_4653at 5383.650 kb on - strand, within Pf6N2E2_4653at 5383.650 kb on - strand, within Pf6N2E2_4653at 5383.779 kb on - strand, within Pf6N2E2_4653at 5383.779 kb on - strand, within Pf6N2E2_4653at 5383.779 kb on - strand, within Pf6N2E2_4653at 5384.040 kb on - strandat 5384.040 kb on - strandat 5384.040 kb on - strandat 5384.124 kb on - strandat 5384.124 kb on - strandat 5384.132 kb on - strandat 5384.152 kb on - strandat 5384.165 kb on - strandat 5384.165 kb on - strandat 5384.165 kb on - strandat 5384.222 kb on - strand, within Pf6N2E2_4654at 5384.252 kb on + strand, within Pf6N2E2_4654at 5384.252 kb on + strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.253 kb on - strand, within Pf6N2E2_4654at 5384.295 kb on + strand, within Pf6N2E2_4654at 5384.295 kb on + strand, within Pf6N2E2_4654at 5384.295 kb on + strand, within Pf6N2E2_4654at 5384.295 kb on + strand, within Pf6N2E2_4654at 5384.296 kb on - strand, within Pf6N2E2_4654at 5384.296 kb on - strand, within Pf6N2E2_4654at 5384.296 kb on - strand, within Pf6N2E2_4654at 5384.324 kb on + strand, within Pf6N2E2_4654at 5384.324 kb on + strand, within Pf6N2E2_4654at 5384.324 kb on + strand, within Pf6N2E2_4654at 5384.325 kb on - strand, within Pf6N2E2_4654at 5384.325 kb on - strand, within Pf6N2E2_4654at 5384.387 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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5,383,203 - +0.6
5,383,209 - +1.6
5,383,209 - +0.6
5,383,209 - +0.5
5,383,213 - +2.3
5,383,213 - -0.8
5,383,213 - +2.4
5,383,213 - +0.4
5,383,213 - +0.6
5,383,213 - +0.8
5,383,332 - Pf6N2E2_4653 0.20 +0.9
5,383,332 - Pf6N2E2_4653 0.20 +1.2
5,383,332 - Pf6N2E2_4653 0.20 +0.6
5,383,332 - Pf6N2E2_4653 0.20 +0.3
5,383,332 - Pf6N2E2_4653 0.20 +1.3
5,383,332 - Pf6N2E2_4653 0.20 +0.8
5,383,431 - Pf6N2E2_4653 0.30 +1.0
5,383,431 - Pf6N2E2_4653 0.30 +1.0
5,383,493 - Pf6N2E2_4653 0.36 +1.4
5,383,650 - Pf6N2E2_4653 0.52 +0.9
5,383,650 - Pf6N2E2_4653 0.52 +1.3
5,383,650 - Pf6N2E2_4653 0.52 +0.7
5,383,650 - Pf6N2E2_4653 0.52 -0.0
5,383,650 - Pf6N2E2_4653 0.52 -0.4
5,383,779 - Pf6N2E2_4653 0.65 +0.7
5,383,779 - Pf6N2E2_4653 0.65 +0.9
5,383,779 - Pf6N2E2_4653 0.65 +1.2
5,384,040 - +3.8
5,384,040 - +3.7
5,384,040 - +3.9
5,384,124 - +4.0
5,384,124 - +4.9
5,384,132 - +2.4
5,384,152 - +2.1
5,384,165 - +1.3
5,384,165 - +2.0
5,384,165 - +2.6
5,384,222 - Pf6N2E2_4654 0.27 +3.5
5,384,252 + Pf6N2E2_4654 0.40 +1.7
5,384,252 + Pf6N2E2_4654 0.40 +0.3
5,384,253 - Pf6N2E2_4654 0.41 +2.6
5,384,253 - Pf6N2E2_4654 0.41 +4.7
5,384,253 - Pf6N2E2_4654 0.41 +4.7
5,384,253 - Pf6N2E2_4654 0.41 +3.4
5,384,253 - Pf6N2E2_4654 0.41 +3.9
5,384,253 - Pf6N2E2_4654 0.41 +3.5
5,384,253 - Pf6N2E2_4654 0.41 +4.9
5,384,253 - Pf6N2E2_4654 0.41 +4.0
5,384,295 + Pf6N2E2_4654 0.59 +0.4
5,384,295 + Pf6N2E2_4654 0.59 -0.9
5,384,295 + Pf6N2E2_4654 0.59 +0.0
5,384,295 + Pf6N2E2_4654 0.59 -1.0
5,384,296 - Pf6N2E2_4654 0.60 +5.4
5,384,296 - Pf6N2E2_4654 0.60 +5.1
5,384,296 - Pf6N2E2_4654 0.60 +6.0
5,384,324 + Pf6N2E2_4654 0.73 -0.2
5,384,324 + Pf6N2E2_4654 0.73 -0.3
5,384,324 + Pf6N2E2_4654 0.73 -0.2
5,384,325 - Pf6N2E2_4654 0.73 +5.1
5,384,325 - Pf6N2E2_4654 0.73 +1.9
5,384,387 + +1.5

Or see this region's nucleotide sequence