Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotides Pf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355
_463
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Position (kb)
528
529
530 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.921 kb on - strand, within Pf6N2E2_462 at 527.935 kb on + strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.188 kb on - strand, within Pf6N2E2_462 at 528.337 kb on + strand, within Pf6N2E2_462 at 528.338 kb on - strand, within Pf6N2E2_462 at 528.391 kb on - strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.548 kb on + strand at 528.711 kb on + strand at 528.712 kb on - strand at 528.948 kb on + strand, within Pf6N2E2_463 at 528.948 kb on + strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.995 kb on + strand, within Pf6N2E2_463 at 529.131 kb on + strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.406 kb on - strand at 529.420 kb on + strand at 529.421 kb on - strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.472 kb on - strand at 529.484 kb on - strand at 529.774 kb on + strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 527,677 + Pf6N2E2_462 0.32 -0.2 527,677 + Pf6N2E2_462 0.32 +0.1 527,677 + Pf6N2E2_462 0.32 +0.8 527,677 + Pf6N2E2_462 0.32 -0.1 527,677 + Pf6N2E2_462 0.32 -0.1 527,677 + Pf6N2E2_462 0.32 -0.2 527,677 + Pf6N2E2_462 0.32 -0.8 527,677 + Pf6N2E2_462 0.32 -0.4 527,678 - Pf6N2E2_462 0.32 +0.6 527,678 - Pf6N2E2_462 0.32 -0.2 527,678 - Pf6N2E2_462 0.32 +0.4 527,678 - Pf6N2E2_462 0.32 +1.8 527,678 - Pf6N2E2_462 0.32 +0.0 527,678 - Pf6N2E2_462 0.32 -0.2 527,678 - Pf6N2E2_462 0.32 -0.2 527,678 - Pf6N2E2_462 0.32 -0.7 527,678 - Pf6N2E2_462 0.32 +0.1 527,686 + Pf6N2E2_462 0.32 -0.3 527,686 + Pf6N2E2_462 0.32 +0.3 527,686 + Pf6N2E2_462 0.32 +0.4 527,686 + Pf6N2E2_462 0.32 -0.1 527,686 + Pf6N2E2_462 0.32 +0.3 527,686 + Pf6N2E2_462 0.32 +0.6 527,686 + Pf6N2E2_462 0.32 +1.7 527,687 - Pf6N2E2_462 0.32 -0.8 527,687 - Pf6N2E2_462 0.32 +0.9 527,687 - Pf6N2E2_462 0.32 -0.7 527,687 - Pf6N2E2_462 0.32 +0.6 527,687 - Pf6N2E2_462 0.32 +1.0 527,687 - Pf6N2E2_462 0.32 -0.7 527,687 - Pf6N2E2_462 0.32 -0.7 527,687 - Pf6N2E2_462 0.32 +1.2 527,921 - Pf6N2E2_462 0.52 -2.0 527,935 + Pf6N2E2_462 0.53 -0.8 527,936 - Pf6N2E2_462 0.54 +0.2 527,936 - Pf6N2E2_462 0.54 +1.1 528,096 + Pf6N2E2_462 0.67 +1.2 528,096 + Pf6N2E2_462 0.67 +0.1 528,096 + Pf6N2E2_462 0.67 +0.5 528,096 + Pf6N2E2_462 0.67 +0.9 528,096 + Pf6N2E2_462 0.67 -1.2 528,097 - Pf6N2E2_462 0.67 -1.1 528,097 - Pf6N2E2_462 0.67 +2.4 528,097 - Pf6N2E2_462 0.67 +1.1 528,097 - Pf6N2E2_462 0.67 +0.2 528,097 - Pf6N2E2_462 0.67 +0.9 528,097 - Pf6N2E2_462 0.67 +1.0 528,097 - Pf6N2E2_462 0.67 +1.0 528,188 - Pf6N2E2_462 0.75 -0.7 528,337 + Pf6N2E2_462 0.87 +1.2 528,338 - Pf6N2E2_462 0.87 +0.7 528,391 - +1.1 528,546 + +1.2 528,546 + +1.4 528,546 + +2.6 528,546 + +1.4 528,546 + +0.3 528,548 + +2.5 528,711 + -3.4 528,712 - -3.4 528,948 + Pf6N2E2_463 0.42 -3.8 528,948 + Pf6N2E2_463 0.42 -4.3 528,949 - Pf6N2E2_463 0.42 -2.0 528,949 - Pf6N2E2_463 0.42 -3.6 528,949 - Pf6N2E2_463 0.42 -2.5 528,949 - Pf6N2E2_463 0.42 -2.2 528,949 - Pf6N2E2_463 0.42 -2.8 528,995 + Pf6N2E2_463 0.49 -2.6 529,131 + Pf6N2E2_463 0.68 -2.6 529,132 - Pf6N2E2_463 0.68 -1.2 529,132 - Pf6N2E2_463 0.68 -1.6 529,171 + Pf6N2E2_463 0.74 -0.2 529,171 + Pf6N2E2_463 0.74 -3.1 529,172 - Pf6N2E2_463 0.74 -2.2 529,172 - Pf6N2E2_463 0.74 -2.9 529,172 - Pf6N2E2_463 0.74 -3.9 529,406 - -3.4 529,420 + -2.0 529,421 - -0.8 529,471 + -2.3 529,471 + -2.2 529,471 + -2.8 529,471 + -1.3 529,471 + -3.8 529,471 + -1.8 529,472 - -2.6 529,484 - -1.6 529,774 + Pf6N2E2_464 0.32 -3.4 529,775 - Pf6N2E2_464 0.32 -2.4 529,775 - Pf6N2E2_464 0.32 -1.7
Or see this region's nucleotide sequence