Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_45

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_44 and Pf6N2E2_45 are separated by 177 nucleotidesPf6N2E2_45 and Pf6N2E2_46 are separated by 54 nucleotides Pf6N2E2_44 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 82,928 to 84,607 _44 Pf6N2E2_45 - Putative protease, at 84,785 to 86,209 _45 Pf6N2E2_46 - membrane protein, putative, at 86,264 to 87,325 _46 Position (kb) 84 85 86 87Strain fitness (log2 ratio) -1 0 1 2at 84.219 kb on + strand, within Pf6N2E2_44at 84.219 kb on + strand, within Pf6N2E2_44at 84.219 kb on + strand, within Pf6N2E2_44at 84.220 kb on - strand, within Pf6N2E2_44at 84.220 kb on - strand, within Pf6N2E2_44at 84.858 kb on + strandat 84.859 kb on - strandat 85.081 kb on - strand, within Pf6N2E2_45at 85.081 kb on - strand, within Pf6N2E2_45at 85.081 kb on - strand, within Pf6N2E2_45at 85.089 kb on + strand, within Pf6N2E2_45at 85.090 kb on - strand, within Pf6N2E2_45at 85.239 kb on + strand, within Pf6N2E2_45at 85.239 kb on + strand, within Pf6N2E2_45at 85.240 kb on - strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.483 kb on + strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.593 kb on + strand, within Pf6N2E2_45at 85.594 kb on - strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.923 kb on + strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 85.924 kb on - strand, within Pf6N2E2_45at 86.010 kb on + strand, within Pf6N2E2_45at 86.028 kb on + strand, within Pf6N2E2_45at 86.028 kb on + strand, within Pf6N2E2_45at 86.230 kb on + strandat 86.230 kb on + strandat 86.230 kb on + strandat 86.230 kb on + strandat 86.231 kb on - strandat 86.231 kb on - strandat 86.247 kb on + strandat 86.247 kb on + strandat 86.506 kb on + strand, within Pf6N2E2_46at 86.506 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.816 kb on - strand, within Pf6N2E2_46at 87.059 kb on + strand, within Pf6N2E2_46at 87.060 kb on - strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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84,219 + Pf6N2E2_44 0.77 +1.2
84,219 + Pf6N2E2_44 0.77 -0.4
84,219 + Pf6N2E2_44 0.77 +1.1
84,220 - Pf6N2E2_44 0.77 -0.3
84,220 - Pf6N2E2_44 0.77 +0.5
84,858 + +0.4
84,859 - +1.6
85,081 - Pf6N2E2_45 0.21 +0.3
85,081 - Pf6N2E2_45 0.21 +0.8
85,081 - Pf6N2E2_45 0.21 -0.3
85,089 + Pf6N2E2_45 0.21 +0.2
85,090 - Pf6N2E2_45 0.21 +0.6
85,239 + Pf6N2E2_45 0.32 -0.1
85,239 + Pf6N2E2_45 0.32 -0.0
85,240 - Pf6N2E2_45 0.32 -0.1
85,344 + Pf6N2E2_45 0.39 +0.9
85,344 + Pf6N2E2_45 0.39 +0.2
85,344 + Pf6N2E2_45 0.39 +0.1
85,344 + Pf6N2E2_45 0.39 -1.0
85,344 + Pf6N2E2_45 0.39 -0.7
85,344 + Pf6N2E2_45 0.39 +2.1
85,344 + Pf6N2E2_45 0.39 +0.1
85,345 - Pf6N2E2_45 0.39 +0.8
85,345 - Pf6N2E2_45 0.39 +0.7
85,345 - Pf6N2E2_45 0.39 +0.2
85,345 - Pf6N2E2_45 0.39 +1.1
85,345 - Pf6N2E2_45 0.39 +0.6
85,345 - Pf6N2E2_45 0.39 -0.2
85,458 + Pf6N2E2_45 0.47 -1.7
85,458 + Pf6N2E2_45 0.47 -0.4
85,458 + Pf6N2E2_45 0.47 +1.1
85,459 - Pf6N2E2_45 0.47 -0.6
85,459 - Pf6N2E2_45 0.47 -0.3
85,459 - Pf6N2E2_45 0.47 -1.3
85,459 - Pf6N2E2_45 0.47 +0.7
85,459 - Pf6N2E2_45 0.47 +1.1
85,483 + Pf6N2E2_45 0.49 +0.7
85,510 - Pf6N2E2_45 0.51 -0.4
85,510 - Pf6N2E2_45 0.51 -0.9
85,562 + Pf6N2E2_45 0.55 +1.1
85,562 + Pf6N2E2_45 0.55 -0.1
85,562 + Pf6N2E2_45 0.55 +0.3
85,593 + Pf6N2E2_45 0.57 -1.5
85,594 - Pf6N2E2_45 0.57 -1.7
85,923 + Pf6N2E2_45 0.80 -0.6
85,923 + Pf6N2E2_45 0.80 +0.4
85,923 + Pf6N2E2_45 0.80 +0.1
85,923 + Pf6N2E2_45 0.80 -0.4
85,923 + Pf6N2E2_45 0.80 -0.7
85,924 - Pf6N2E2_45 0.80 +0.3
85,924 - Pf6N2E2_45 0.80 +0.2
85,924 - Pf6N2E2_45 0.80 +1.4
86,010 + Pf6N2E2_45 0.86 +0.1
86,028 + Pf6N2E2_45 0.87 -0.1
86,028 + Pf6N2E2_45 0.87 +0.2
86,230 + +1.0
86,230 + -0.1
86,230 + +0.7
86,230 + +0.1
86,231 - +0.9
86,231 - -0.5
86,247 + -0.1
86,247 + -0.3
86,506 + Pf6N2E2_46 0.23 -0.5
86,506 + Pf6N2E2_46 0.23 +0.2
86,537 + Pf6N2E2_46 0.26 +0.7
86,537 + Pf6N2E2_46 0.26 -0.7
86,538 - Pf6N2E2_46 0.26 +0.4
86,538 - Pf6N2E2_46 0.26 -0.1
86,771 + Pf6N2E2_46 0.48 +0.6
86,771 + Pf6N2E2_46 0.48 -1.0
86,772 - Pf6N2E2_46 0.48 +1.0
86,772 - Pf6N2E2_46 0.48 -0.4
86,816 - Pf6N2E2_46 0.52 +0.5
87,059 + Pf6N2E2_46 0.75 +0.4
87,060 - Pf6N2E2_46 0.75 -0.6
87,185 + Pf6N2E2_46 0.87 +0.2
87,185 + Pf6N2E2_46 0.87 +0.6
87,185 + Pf6N2E2_46 0.87 -0.7
87,185 + Pf6N2E2_46 0.87 +0.8
87,185 + Pf6N2E2_46 0.87 +0.8
87,186 - Pf6N2E2_46 0.87 +1.5
87,186 - Pf6N2E2_46 0.87 +1.1
87,186 - Pf6N2E2_46 0.87 -0.3

Or see this region's nucleotide sequence