Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4336 and Pf6N2E2_4337 are separated by 415 nucleotides Pf6N2E2_4337 and Pf6N2E2_4338 are separated by 68 nucleotides Pf6N2E2_4338 and Pf6N2E2_4339 are separated by 15 nucleotides
Pf6N2E2_4336 - Uncharacterized protein ImpA, at 5,058,260 to 5,059,303
_4336
Pf6N2E2_4337 - Uncharacterized protein ImpI/VasC, at 5,059,719 to 5,061,218
_4337
Pf6N2E2_4338 - Type VI secretion lipoprotein/VasD, at 5,061,287 to 5,061,793
_4338
Pf6N2E2_4339 - Uncharacterized protein ImpJ/VasE, at 5,061,809 to 5,063,143
_4339
Position (kb) 5059
5060
5061
5062 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5058.996 kb on + strand, within Pf6N2E2_4336 at 5058.996 kb on + strand, within Pf6N2E2_4336 at 5058.996 kb on + strand, within Pf6N2E2_4336 at 5058.997 kb on - strand, within Pf6N2E2_4336 at 5058.997 kb on - strand, within Pf6N2E2_4336 at 5059.239 kb on + strand at 5059.240 kb on - strand at 5059.399 kb on + strand at 5059.400 kb on - strand at 5059.434 kb on - strand at 5059.460 kb on + strand at 5059.463 kb on + strand at 5059.464 kb on - strand at 5059.464 kb on - strand at 5059.464 kb on - strand at 5059.464 kb on - strand at 5059.464 kb on - strand at 5059.464 kb on - strand at 5059.468 kb on - strand at 5059.482 kb on + strand at 5059.483 kb on - strand at 5059.483 kb on - strand at 5059.483 kb on - strand at 5059.487 kb on + strand at 5059.487 kb on + strand at 5059.488 kb on - strand at 5059.488 kb on - strand at 5059.488 kb on - strand at 5059.501 kb on - strand at 5059.511 kb on + strand at 5059.511 kb on + strand at 5059.512 kb on - strand at 5059.596 kb on - strand at 5059.596 kb on - strand at 5059.700 kb on + strand at 5059.701 kb on - strand at 5059.736 kb on + strand at 5059.736 kb on + strand at 5059.736 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.742 kb on + strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.743 kb on - strand at 5059.746 kb on + strand at 5059.746 kb on + strand at 5059.747 kb on - strand at 5059.747 kb on - strand at 5059.747 kb on - strand at 5059.747 kb on - strand at 5059.834 kb on + strand at 5059.884 kb on + strand, within Pf6N2E2_4337 at 5059.884 kb on + strand, within Pf6N2E2_4337 at 5059.884 kb on + strand, within Pf6N2E2_4337 at 5059.885 kb on - strand, within Pf6N2E2_4337 at 5059.900 kb on - strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.902 kb on + strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.903 kb on - strand, within Pf6N2E2_4337 at 5059.907 kb on - strand, within Pf6N2E2_4337 at 5059.914 kb on - strand, within Pf6N2E2_4337 at 5059.922 kb on + strand, within Pf6N2E2_4337 at 5060.013 kb on + strand, within Pf6N2E2_4337 at 5060.013 kb on + strand, within Pf6N2E2_4337 at 5060.013 kb on + strand, within Pf6N2E2_4337 at 5060.014 kb on - strand, within Pf6N2E2_4337 at 5060.144 kb on + strand, within Pf6N2E2_4337 at 5060.144 kb on + strand, within Pf6N2E2_4337 at 5060.144 kb on + strand, within Pf6N2E2_4337 at 5060.144 kb on + strand, within Pf6N2E2_4337 at 5060.145 kb on - strand, within Pf6N2E2_4337 at 5060.393 kb on + strand, within Pf6N2E2_4337 at 5060.731 kb on - strand, within Pf6N2E2_4337 at 5061.104 kb on + strand at 5061.105 kb on - strand at 5061.115 kb on - strand at 5061.115 kb on - strand at 5061.115 kb on - strand at 5061.115 kb on - strand at 5061.183 kb on + strand at 5061.260 kb on + strand at 5061.261 kb on - strand at 5061.638 kb on - strand, within Pf6N2E2_4338 at 5061.645 kb on + strand, within Pf6N2E2_4338 at 5061.646 kb on - strand, within Pf6N2E2_4338 at 5061.713 kb on + strand, within Pf6N2E2_4338 at 5061.714 kb on - strand, within Pf6N2E2_4338 at 5061.723 kb on - strand, within Pf6N2E2_4338 at 5062.103 kb on + strand, within Pf6N2E2_4339 at 5062.104 kb on - strand, within Pf6N2E2_4339 at 5062.104 kb on - strand, within Pf6N2E2_4339 at 5062.104 kb on - strand, within Pf6N2E2_4339 at 5062.104 kb on - strand, within Pf6N2E2_4339 at 5062.178 kb on + strand, within Pf6N2E2_4339 at 5062.178 kb on + strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339 at 5062.179 kb on - strand, within Pf6N2E2_4339
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,058,996 + Pf6N2E2_4336 0.70 +0.2 5,058,996 + Pf6N2E2_4336 0.70 +1.5 5,058,996 + Pf6N2E2_4336 0.70 -0.2 5,058,997 - Pf6N2E2_4336 0.71 +0.9 5,058,997 - Pf6N2E2_4336 0.71 +1.3 5,059,239 + -1.2 5,059,240 - +1.5 5,059,399 + -0.2 5,059,400 - +0.1 5,059,434 - -0.0 5,059,460 + -0.6 5,059,463 + +0.6 5,059,464 - +0.3 5,059,464 - +1.4 5,059,464 - +1.6 5,059,464 - +1.2 5,059,464 - -0.1 5,059,464 - +0.9 5,059,468 - +1.6 5,059,482 + +0.4 5,059,483 - +1.0 5,059,483 - -0.0 5,059,483 - -0.3 5,059,487 + -0.1 5,059,487 + +0.7 5,059,488 - +0.6 5,059,488 - -1.0 5,059,488 - +0.8 5,059,501 - +1.4 5,059,511 + -0.8 5,059,511 + -1.0 5,059,512 - +0.7 5,059,596 - -0.1 5,059,596 - +1.0 5,059,700 + -0.1 5,059,701 - +0.2 5,059,736 + -0.1 5,059,736 + -0.2 5,059,736 + -0.1 5,059,742 + +0.0 5,059,742 + -1.2 5,059,742 + +0.8 5,059,742 + +0.8 5,059,742 + +1.2 5,059,742 + +0.3 5,059,742 + +0.7 5,059,742 + +0.5 5,059,742 + -0.2 5,059,742 + +0.7 5,059,742 + +0.9 5,059,743 - -1.0 5,059,743 - +1.4 5,059,743 - +0.7 5,059,743 - +1.2 5,059,743 - -0.7 5,059,743 - -1.9 5,059,743 - +1.0 5,059,743 - +0.4 5,059,743 - +0.1 5,059,746 + +0.6 5,059,746 + +0.8 5,059,747 - +0.1 5,059,747 - +0.4 5,059,747 - -0.5 5,059,747 - +1.7 5,059,834 + +0.6 5,059,884 + Pf6N2E2_4337 0.11 +1.2 5,059,884 + Pf6N2E2_4337 0.11 +2.2 5,059,884 + Pf6N2E2_4337 0.11 +2.5 5,059,885 - Pf6N2E2_4337 0.11 +0.0 5,059,900 - Pf6N2E2_4337 0.12 +0.4 5,059,902 + Pf6N2E2_4337 0.12 -0.0 5,059,902 + Pf6N2E2_4337 0.12 +0.6 5,059,902 + Pf6N2E2_4337 0.12 +1.5 5,059,902 + Pf6N2E2_4337 0.12 +0.4 5,059,902 + Pf6N2E2_4337 0.12 +1.1 5,059,902 + Pf6N2E2_4337 0.12 +1.0 5,059,902 + Pf6N2E2_4337 0.12 +0.2 5,059,902 + Pf6N2E2_4337 0.12 +0.4 5,059,902 + Pf6N2E2_4337 0.12 -0.4 5,059,903 - Pf6N2E2_4337 0.12 +0.5 5,059,903 - Pf6N2E2_4337 0.12 -0.2 5,059,903 - Pf6N2E2_4337 0.12 +0.7 5,059,903 - Pf6N2E2_4337 0.12 +0.9 5,059,903 - Pf6N2E2_4337 0.12 +1.6 5,059,903 - Pf6N2E2_4337 0.12 +0.9 5,059,903 - Pf6N2E2_4337 0.12 +1.0 5,059,903 - Pf6N2E2_4337 0.12 +1.2 5,059,903 - Pf6N2E2_4337 0.12 +0.6 5,059,903 - Pf6N2E2_4337 0.12 +0.6 5,059,907 - Pf6N2E2_4337 0.13 +0.8 5,059,914 - Pf6N2E2_4337 0.13 +1.1 5,059,922 + Pf6N2E2_4337 0.14 -0.1 5,060,013 + Pf6N2E2_4337 0.20 +1.1 5,060,013 + Pf6N2E2_4337 0.20 -0.4 5,060,013 + Pf6N2E2_4337 0.20 +1.6 5,060,014 - Pf6N2E2_4337 0.20 +1.2 5,060,144 + Pf6N2E2_4337 0.28 +0.0 5,060,144 + Pf6N2E2_4337 0.28 +0.0 5,060,144 + Pf6N2E2_4337 0.28 +0.8 5,060,144 + Pf6N2E2_4337 0.28 +0.1 5,060,145 - Pf6N2E2_4337 0.28 +0.5 5,060,393 + Pf6N2E2_4337 0.45 +0.5 5,060,731 - Pf6N2E2_4337 0.67 +0.9 5,061,104 + -1.5 5,061,105 - +1.4 5,061,115 - +0.2 5,061,115 - +0.7 5,061,115 - +0.7 5,061,115 - +0.6 5,061,183 + -1.6 5,061,260 + +0.1 5,061,261 - +1.3 5,061,638 - Pf6N2E2_4338 0.69 -0.2 5,061,645 + Pf6N2E2_4338 0.71 -0.9 5,061,646 - Pf6N2E2_4338 0.71 +1.3 5,061,713 + Pf6N2E2_4338 0.84 +0.4 5,061,714 - Pf6N2E2_4338 0.84 +0.4 5,061,723 - Pf6N2E2_4338 0.86 +0.5 5,062,103 + Pf6N2E2_4339 0.22 +1.1 5,062,104 - Pf6N2E2_4339 0.22 +0.0 5,062,104 - Pf6N2E2_4339 0.22 -0.0 5,062,104 - Pf6N2E2_4339 0.22 -0.9 5,062,104 - Pf6N2E2_4339 0.22 +0.6 5,062,178 + Pf6N2E2_4339 0.28 +0.2 5,062,178 + Pf6N2E2_4339 0.28 -0.4 5,062,179 - Pf6N2E2_4339 0.28 +0.3 5,062,179 - Pf6N2E2_4339 0.28 +1.1 5,062,179 - Pf6N2E2_4339 0.28 -0.3 5,062,179 - Pf6N2E2_4339 0.28 +1.0 5,062,179 - Pf6N2E2_4339 0.28 +0.5 5,062,179 - Pf6N2E2_4339 0.28 +0.7
Or see this region's nucleotide sequence