Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4331 and Pf6N2E2_4332 overlap by 4 nucleotides Pf6N2E2_4332 and Pf6N2E2_4333 are separated by 81 nucleotides Pf6N2E2_4333 and Pf6N2E2_4334 are separated by 220 nucleotides
Pf6N2E2_4331 - hypothetical protein, at 5,054,481 to 5,054,930
_4331
Pf6N2E2_4332 - hypothetical protein, at 5,054,927 to 5,055,382
_4332
Pf6N2E2_4333 - Uncharacterized protein ImpD, at 5,055,464 to 5,055,952
_4333
Pf6N2E2_4334 - Uncharacterized protein ImpC, at 5,056,173 to 5,057,675
_4334
Position (kb)
5055
5056 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5054.503 kb on + strand at 5054.586 kb on + strand, within Pf6N2E2_4331 at 5054.587 kb on - strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.657 kb on + strand, within Pf6N2E2_4331 at 5054.658 kb on - strand, within Pf6N2E2_4331 at 5054.907 kb on + strand at 5054.907 kb on + strand at 5054.908 kb on - strand at 5054.908 kb on - strand at 5054.908 kb on - strand at 5054.930 kb on + strand at 5054.930 kb on + strand at 5054.930 kb on + strand at 5054.931 kb on - strand at 5054.931 kb on - strand at 5054.931 kb on - strand at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.254 kb on - strand, within Pf6N2E2_4332 at 5055.325 kb on - strand, within Pf6N2E2_4332 at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.346 kb on - strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.392 kb on - strand at 5055.392 kb on - strand at 5055.465 kb on + strand at 5055.465 kb on + strand at 5055.466 kb on - strand at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.632 kb on - strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.940 kb on + strand at 5055.975 kb on - strand at 5056.175 kb on - strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.241 kb on - strand at 5056.255 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.271 kb on - strand at 5056.271 kb on - strand at 5056.271 kb on - strand at 5056.273 kb on + strand at 5056.375 kb on + strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.462 kb on + strand, within Pf6N2E2_4334 at 5056.462 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.493 kb on - strand, within Pf6N2E2_4334 at 5056.544 kb on - strand, within Pf6N2E2_4334 at 5056.819 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,054,503 + -2.0 5,054,586 + Pf6N2E2_4331 0.23 +0.8 5,054,587 - Pf6N2E2_4331 0.24 +1.0 5,054,657 + Pf6N2E2_4331 0.39 +0.4 5,054,657 + Pf6N2E2_4331 0.39 -0.2 5,054,657 + Pf6N2E2_4331 0.39 +0.6 5,054,658 - Pf6N2E2_4331 0.39 -0.6 5,054,907 + -0.0 5,054,907 + -0.3 5,054,908 - +1.6 5,054,908 - +1.1 5,054,908 - +0.2 5,054,930 + +0.8 5,054,930 + +1.7 5,054,930 + +0.1 5,054,931 - +0.7 5,054,931 - +0.8 5,054,931 - -0.2 5,055,219 + Pf6N2E2_4332 0.64 +1.2 5,055,219 + Pf6N2E2_4332 0.64 -0.0 5,055,220 - Pf6N2E2_4332 0.64 +0.7 5,055,220 - Pf6N2E2_4332 0.64 +1.0 5,055,253 + Pf6N2E2_4332 0.71 +0.8 5,055,253 + Pf6N2E2_4332 0.71 +0.7 5,055,254 - Pf6N2E2_4332 0.72 +0.2 5,055,325 - Pf6N2E2_4332 0.87 +1.0 5,055,345 + -0.1 5,055,345 + -0.1 5,055,345 + +0.7 5,055,345 + +0.1 5,055,346 - +0.6 5,055,391 + +1.2 5,055,391 + +0.1 5,055,391 + +0.2 5,055,392 - -0.1 5,055,392 - +0.9 5,055,465 + +0.1 5,055,465 + +0.7 5,055,466 - +0.5 5,055,631 + Pf6N2E2_4333 0.34 +0.4 5,055,631 + Pf6N2E2_4333 0.34 +0.3 5,055,632 - Pf6N2E2_4333 0.34 +0.1 5,055,663 + Pf6N2E2_4333 0.41 -0.4 5,055,663 + Pf6N2E2_4333 0.41 +1.8 5,055,663 + Pf6N2E2_4333 0.41 -0.2 5,055,664 - Pf6N2E2_4333 0.41 +0.5 5,055,664 - Pf6N2E2_4333 0.41 +1.7 5,055,771 + Pf6N2E2_4333 0.63 +0.6 5,055,771 + Pf6N2E2_4333 0.63 +0.4 5,055,771 + Pf6N2E2_4333 0.63 +0.6 5,055,772 - Pf6N2E2_4333 0.63 +1.4 5,055,772 - Pf6N2E2_4333 0.63 +1.3 5,055,772 - Pf6N2E2_4333 0.63 +0.4 5,055,772 - Pf6N2E2_4333 0.63 +0.5 5,055,772 - Pf6N2E2_4333 0.63 -0.1 5,055,772 - Pf6N2E2_4333 0.63 +0.5 5,055,772 - Pf6N2E2_4333 0.63 -0.2 5,055,940 + -0.2 5,055,975 - +0.6 5,056,175 - +0.3 5,056,240 + -0.1 5,056,240 + -0.1 5,056,240 + +0.6 5,056,240 + +0.5 5,056,241 - +1.6 5,056,255 + -1.0 5,056,270 + -0.8 5,056,270 + +0.3 5,056,270 + +0.1 5,056,270 + +0.1 5,056,271 - -0.1 5,056,271 - +0.4 5,056,271 - +1.1 5,056,273 + +0.6 5,056,375 + Pf6N2E2_4334 0.13 +0.6 5,056,376 - Pf6N2E2_4334 0.14 +0.2 5,056,376 - Pf6N2E2_4334 0.14 -0.4 5,056,376 - Pf6N2E2_4334 0.14 -0.7 5,056,376 - Pf6N2E2_4334 0.14 +0.6 5,056,462 + Pf6N2E2_4334 0.19 -0.4 5,056,462 + Pf6N2E2_4334 0.19 +0.5 5,056,492 + Pf6N2E2_4334 0.21 +1.3 5,056,492 + Pf6N2E2_4334 0.21 +0.9 5,056,492 + Pf6N2E2_4334 0.21 -0.2 5,056,493 - Pf6N2E2_4334 0.21 +1.1 5,056,544 - Pf6N2E2_4334 0.25 -0.1 5,056,819 + Pf6N2E2_4334 0.43 +0.4 5,056,840 + Pf6N2E2_4334 0.44 +0.8 5,056,840 + Pf6N2E2_4334 0.44 +1.2 5,056,840 + Pf6N2E2_4334 0.44 -0.3
Or see this region's nucleotide sequence