Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4304 and Pf6N2E2_4305 are separated by 57 nucleotides Pf6N2E2_4305 and Pf6N2E2_4306 are separated by 19 nucleotides Pf6N2E2_4306 and Pf6N2E2_4307 are separated by 5 nucleotides
Pf6N2E2_4304 - Sigma-54 dependent transcriptional regulator, at 5,020,452 to 5,021,969
_4304
Pf6N2E2_4305 - FIG00959590: hypothetical protein, at 5,022,027 to 5,022,167
_4305
Pf6N2E2_4306 - Uncharacterized protein ImpI/VasC, at 5,022,187 to 5,023,032
_4306
Pf6N2E2_4307 - Type VI secretion lipoprotein/VasD, at 5,023,038 to 5,023,541
_4307
Position (kb) 5022
5023 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5021.031 kb on + strand, within Pf6N2E2_4304 at 5021.032 kb on - strand, within Pf6N2E2_4304 at 5021.045 kb on + strand, within Pf6N2E2_4304 at 5021.046 kb on - strand, within Pf6N2E2_4304 at 5021.085 kb on - strand, within Pf6N2E2_4304 at 5021.104 kb on - strand, within Pf6N2E2_4304 at 5021.195 kb on + strand, within Pf6N2E2_4304 at 5021.195 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.511 kb on + strand, within Pf6N2E2_4304 at 5021.512 kb on - strand, within Pf6N2E2_4304 at 5021.512 kb on - strand, within Pf6N2E2_4304 at 5021.512 kb on - strand, within Pf6N2E2_4304 at 5021.710 kb on + strand, within Pf6N2E2_4304 at 5021.710 kb on + strand, within Pf6N2E2_4304 at 5021.711 kb on - strand, within Pf6N2E2_4304 at 5021.768 kb on - strand, within Pf6N2E2_4304 at 5021.806 kb on + strand, within Pf6N2E2_4304 at 5021.806 kb on + strand, within Pf6N2E2_4304 at 5021.807 kb on - strand, within Pf6N2E2_4304 at 5021.922 kb on + strand at 5021.922 kb on + strand at 5022.093 kb on - strand, within Pf6N2E2_4305 at 5022.124 kb on - strand, within Pf6N2E2_4305 at 5022.162 kb on - strand at 5022.180 kb on + strand at 5022.180 kb on + strand at 5022.181 kb on - strand at 5022.184 kb on + strand at 5022.184 kb on + strand at 5022.184 kb on + strand at 5022.184 kb on + strand at 5022.184 kb on + strand at 5022.185 kb on - strand at 5022.185 kb on - strand at 5022.185 kb on - strand at 5022.185 kb on - strand at 5022.185 kb on - strand at 5022.233 kb on + strand at 5022.234 kb on - strand at 5022.420 kb on - strand, within Pf6N2E2_4306 at 5022.450 kb on - strand, within Pf6N2E2_4306 at 5022.466 kb on + strand, within Pf6N2E2_4306 at 5022.466 kb on + strand, within Pf6N2E2_4306 at 5022.466 kb on + strand, within Pf6N2E2_4306 at 5022.467 kb on - strand, within Pf6N2E2_4306 at 5022.467 kb on - strand, within Pf6N2E2_4306 at 5022.467 kb on - strand, within Pf6N2E2_4306 at 5022.467 kb on - strand, within Pf6N2E2_4306 at 5022.691 kb on + strand, within Pf6N2E2_4306 at 5022.691 kb on + strand, within Pf6N2E2_4306 at 5022.699 kb on + strand, within Pf6N2E2_4306 at 5022.699 kb on + strand, within Pf6N2E2_4306 at 5022.979 kb on + strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand at 5022.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,021,031 + Pf6N2E2_4304 0.38 +0.1 5,021,032 - Pf6N2E2_4304 0.38 -2.4 5,021,045 + Pf6N2E2_4304 0.39 +0.1 5,021,046 - Pf6N2E2_4304 0.39 -1.9 5,021,085 - Pf6N2E2_4304 0.42 +0.8 5,021,104 - Pf6N2E2_4304 0.43 +0.4 5,021,195 + Pf6N2E2_4304 0.49 +0.6 5,021,195 + Pf6N2E2_4304 0.49 -1.4 5,021,511 + Pf6N2E2_4304 0.70 +0.8 5,021,511 + Pf6N2E2_4304 0.70 -0.3 5,021,511 + Pf6N2E2_4304 0.70 +0.9 5,021,511 + Pf6N2E2_4304 0.70 +0.0 5,021,511 + Pf6N2E2_4304 0.70 +0.1 5,021,511 + Pf6N2E2_4304 0.70 +0.3 5,021,512 - Pf6N2E2_4304 0.70 -0.5 5,021,512 - Pf6N2E2_4304 0.70 -0.9 5,021,512 - Pf6N2E2_4304 0.70 -0.1 5,021,710 + Pf6N2E2_4304 0.83 +0.4 5,021,710 + Pf6N2E2_4304 0.83 +1.0 5,021,711 - Pf6N2E2_4304 0.83 +0.3 5,021,768 - Pf6N2E2_4304 0.87 -0.9 5,021,806 + Pf6N2E2_4304 0.89 -0.0 5,021,806 + Pf6N2E2_4304 0.89 -1.1 5,021,807 - Pf6N2E2_4304 0.89 +1.4 5,021,922 + -1.4 5,021,922 + -0.5 5,022,093 - Pf6N2E2_4305 0.47 -0.1 5,022,124 - Pf6N2E2_4305 0.69 -2.4 5,022,162 - +1.1 5,022,180 + +0.1 5,022,180 + +1.2 5,022,181 - -0.3 5,022,184 + -1.1 5,022,184 + +0.5 5,022,184 + -1.2 5,022,184 + -0.2 5,022,184 + +0.2 5,022,185 - +0.1 5,022,185 - +0.4 5,022,185 - +0.1 5,022,185 - -0.3 5,022,185 - -0.7 5,022,233 + -2.5 5,022,234 - -0.9 5,022,420 - Pf6N2E2_4306 0.28 -0.3 5,022,450 - Pf6N2E2_4306 0.31 +0.6 5,022,466 + Pf6N2E2_4306 0.33 -0.2 5,022,466 + Pf6N2E2_4306 0.33 +0.4 5,022,466 + Pf6N2E2_4306 0.33 +0.8 5,022,467 - Pf6N2E2_4306 0.33 +0.8 5,022,467 - Pf6N2E2_4306 0.33 +0.9 5,022,467 - Pf6N2E2_4306 0.33 -1.0 5,022,467 - Pf6N2E2_4306 0.33 +0.6 5,022,691 + Pf6N2E2_4306 0.60 +0.4 5,022,691 + Pf6N2E2_4306 0.60 -0.6 5,022,699 + Pf6N2E2_4306 0.61 +0.6 5,022,699 + Pf6N2E2_4306 0.61 -0.5 5,022,979 + -0.2 5,022,980 - -3.6 5,022,980 - -0.2 5,022,980 - +1.1 5,022,980 - -0.2 5,022,980 - +0.3 5,022,980 - +0.9
Or see this region's nucleotide sequence